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Quantitative phase imaging (QPI) has been widely applied in characterizing cells and tissues. Spatial light interference microscopy (SLIM) is a highly sensitive QPI method, due to its partially coherent illumination and common path…

Image and Video Processing · Electrical Eng. & Systems 2024-06-12 Yuheng Jiao , Yuchen R. He , Mikhail E. Kandel , Xiaojun Liu , Wenlong Lu , Gabriel Popescu

Quantitative phase imaging (QPI) quantifies the sample-specific optical-phase-delay enabling objective studies of optically-transparent specimens such as biological samples, but lacks chemical sensitivity limiting its application to…

Optics · Physics 2019-09-04 Miu Tamamitsu , Keiichiro Toda , Ryoichi Horisaki , Takuro Ideguchi

Quadriwave lateral shearing interferometry (QLSI) is a quantitative phase imaging technique based on the use of a diffraction grating placed in front of a camera. This grating creates a wire-mesh-like image, called an interferogram, that is…

Optics · Physics 2022-03-15 Guillaume Baffou

Quantitative phase microscopy (QPM), a technique combining phase imaging and microscopy, enables visualization of the 3D topography in reflective samples, as well as the inner structure or refractive index distribution of transparent and…

Optics · Physics 2025-01-17 Vicente Mico , Juanjuan Zheng , Javier Garcia , Zeev Zalevsky , Peng Gao

In this paper, we review spatial light interference microscopy (SLIM), a common-path, phase-shifting interferometer, built onto a phase-contrast microscope, with white-light illumination. As one of the most sensitive quantitative phase…

Optics · Physics 2020-12-17 Xi Chen , Mikhail E. Kandel , Gabriel Popescu

White light phase-shifting interference microscopy (WL-PSIM) is a prominent technique for high-resolution quantitative phase imaging (QPI) of industrial and biological specimens. However, multiple interferograms with accurate phase-shifts…

Image and Video Processing · Electrical Eng. & Systems 2021-05-04 Sunil Bhatt , Ankit Butola , Sheetal Raosaheb Kanade , Anand Kumar , Dalip Singh Mehta

This article presents a thorough comparison of themain QPM techniques, focusing on their accuracy in terms of measurement precision and trueness. We focus on 8 techniques, namely digital holographic microscopy (DHM), cross-grating wavefront…

Quantitative phase imaging (QPI) is a label-free computational imaging technique used in various fields, including biology and medical research. Modern QPI systems typically rely on digital processing using iterative algorithms for phase…

Optics · Physics 2023-06-28 Yuhang Li , Yi Luo , Deniz Mengu , Bijie Bai , Aydogan Ozcan

Quantitative phase imaging (QPI) is important in many applications such as microscopy and crystallography. To quantitatively reveal phase information, people could either employ interference to map phase distribution into intensity fringes,…

Optics · Physics 2020-11-11 Xianye Li , Yafei sun , Yikang He , Xun Li , Baoqing Sun

As a label-free imaging technique, quantitative phase imaging (QPI) provides optical path length information of transparent specimens for various applications in biology, materials science, and engineering. Multispectral QPI measures…

Optics · Physics 2023-08-29 Che-Yung Shen , Jingxi Li , Deniz Mengu , Aydogan Ozcan

Sub-diffraction resolution imaging has played a pivotal role in biological research by visualizing key, but previously unresolvable, sub-cellular structures. Unfortunately, applications of far-field sub-diffraction resolution are currently…

Biological Physics · Physics 2017-05-02 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

Quantitative phase imaging (QPI) is a label-free technique that provides optical path length information for transparent specimens, finding utility in biology, materials science, and engineering. Here, we present quantitative phase imaging…

Quantitative phase imaging (QPI) enables visualization and quantitative extraction of the optical phase information of transparent samples. However, conventional QPI techniques typically rely on multi-frame acquisition or complex…

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

Multi-spectral quantitative phase imaging (MS-QPI) is a cutting-edge label-free technique to determine the morphological changes, refractive index variations and spectroscopic information of the specimens. The bottleneck to implement this…

Quantitative susceptibility mapping (QSM) has gained broad interests in the field by extracting biological tissue properties, predominantly myelin, iron and calcium from magnetic resonance imaging (MRI) phase measurements in vivo. Thereby,…

Image and Video Processing · Electrical Eng. & Systems 2019-12-12 Woojin Jung , Steffen Bollmann , Jongho Lee

Quantitative susceptibility mapping (QSM) utilizes MRI signal phase to estimate local tissue susceptibility, which has been shown useful to provide novel image contrast and as biomarkers of abnormal tissue. QSM requires addressing a…

Medical Physics · Physics 2019-06-03 Juan Liu , Kevin M. Koch

High space-bandwidth product with high spatial phase sensitivity is indispensable for a single-shot quantitative phase microscopy (QPM) system. It opens avenue for widespread applications of QPM in the field of biomedical imaging.…

In this letter, a high-fidelity single-shot differential quantitative phase microscopy (dQPM) method is presented to effectively image nearly transparent biological samples. The proposed method is based on a common-path Sagnac…

Quantitative phase imaging (QPI) is a valuable label-free modality that has gained significant interest due to its wide potentials, from basic biology to clinical applications. Most existing QPI systems measure microscopic objects via…

Optics · Physics 2021-12-22 Chenfei Hu , Mikhail E. Kandel , Young Jae Lee , Gabriel Popescu
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