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The mode of action of proteins is to a large extent given by their ability to adopt different conformations. This is why imaging single biomolecules at atomic resolution is one of the ultimate goals of biophysics and structural biology. The…

Biological Physics · Physics 2012-09-27 Jean-Nicolas Longchamp , Tatiana Latychevskaia , Conrad Escher , Hans-Werner Fink

Single-atom-resolved detection in optical lattices using quantum-gas microscopes has enabled a new generation of experiments in the field of quantum simulation. Fluorescence imaging of individual atoms has so far been achieved for bosonic…

Solid-state nanopores are emerging platforms for single-molecule protein sequencing due to their tolerance to hash physiology environment and compatibility with different electrical and optical detection methods. However, they suffer from…

Applied Physics · Physics 2024-01-05 Yingqi Zhao , Aliaksandr Hubarevich , Jian-An Huang , Francesco De Angelis

Single particle-resolved fluorescence imaging is an enabling technology in cold-atom physics. However, so far, this technique was not available for nanophotonic atom-light interfaces. Here, we image single atoms that are trapped and…

Protein identification and profiling is critical for the advancement of cell and molecular biology as well as medical diagnostics. Although mass spectrometry and protein microarrays are commonly used for protein identification, both methods…

Recent advances in top-down mass spectrometry enabled identification of intact proteins, but this technology still faces challenges. For example, top-down mass spectrometry suffers from a lack of sensitivity since the ion counts for a…

Quantitative Methods · Quantitative Biology 2017-07-05 Mikhail Kolmogorov , Eamonn Kennedy , Zhuxin Dong , Gregory Timp , Pavel Pevzner

In cold atomic systems, fast and high-resolution microscopy of individual atoms is crucial, since it can provide direct information on the dynamics and correlations of the system. Here, we demonstrate nanosecond-scale two-dimensional…

We report on image processing techniques and experimental procedures to determine the lattice-site positions of single atoms in an optical lattice with high reliability, even for limited acquisition time or optical resolution. Determining…

The clinical need for ultra-sensitive molecular analysis has motivated the development of several endpoint assay technologies capable of single molecule readout. These endpoint assays are now primarily limited by the affinity and…

Biological Physics · Physics 2019-05-22 Derin Sevenler , Jacob Trueb , M. Selim Ünlü

Super-resolution imaging based on single molecule localization allows accessing nanometric-scale information in biological samples with high precision. However, complete measurements including molecule orientation are still challenging.…

We experimentally demonstrate a detection scheme suitable for state analysis of single optically trapped atoms in less than 1 {\mu}s with an overall detection efficiency {\eta} exceeding 98%. The method is based on hyperfine-state-selective…

As the ultimate miniaturization of semiconductor devices approaches, it is imperative that the effects of single dopants be clarified. Beyond providing insight into functions and limitations of conventional devices, such information enables…

Mesoscale and Nanoscale Physics · Physics 2016-10-27 Mohammad Rashidi , Jacob Burgess , Marco Taucer , Roshan Achal , Jason L. Pitters , Sebastian Loth , Robert A. Wolkow

Single molecule detection has revolutionised the fields of chemistry and biology by offering powerful ways to study individual molecules under different scenarios. Nanophotonic structures, including plasmonic antennas, significantly…

Biological Physics · Physics 2022-01-31 Pamina M. Winkler , Thomas S. van Zanten , Satyajit Mayor , Maria F. Garcia-Parajo

High-Q optical resonators allow label-free detection of individual nanoparticles through perturbation of optical signatures but have practical limitations due to reliance on random diffusion to deliver particles to the sensing region. We…

Optics · Physics 2016-10-03 Jeewon Suh , Kewen Han , Christopher W Peterson , Gaurav Bahl

In this paper we propose a new method for measuring the cross section of low yield nuclear reactions by capturing the products in a cryogenically frozen noble gas solid. Once embedded in the noble gas solid, which is optically transparent,…

Instrumentation and Detectors · Physics 2019-06-19 Benjamin Loseth , Ruoyu Fang , Dustin Frisbie , Kristen Parzuchowski , Claudio Ugalde , Jennifer Wenzl , Jaideep Taggart Singh

Optical molecular sensing techniques are often limited by the refractive index change associated with the probed interactions. In this work, we present a closed form analytical model to estimate the magnitude of optical refractive index…

Applied Physics · Physics 2017-12-04 Harish Sasikumar , Manoj M. Varma

Direct monitoring of singlet oxygen (1O2) luminescence is a particularly challenging infrared photodetection problem. 1O2, an excited state of the oxygen molecule, is a crucial intermediate in many biological processes. We employ a low…

Ultrasensitive optical detection of nanometer-scaled particles is highly desirable for applications in early-stage diagnosis of human diseases, environmental monitoring, and homeland security, but remains extremely difficult due to ultralow…

Droplet microfluidics has emerged as a powerful platform allowing a large number of individual reactions to be carried out in spatially distinct microcompartments. Due to their small size, however, the spectroscopic characterisation of…

The miniaturization of semiconductor devices to the scales where small numbers of dopants can control device properties requires the development of new techniques capable of characterizing their dynamics. Investigating single dopants…

Mesoscale and Nanoscale Physics · Physics 2017-06-28 Mohammad Rashidi , Wyatt Vine , Jacob A. J. Burgess , Marco Taucer , Roshan Achal , Jason L. Pitters , Sebastian Loth , Robert A. Wolkow