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Raman spectroscopy's capability to provide meaningful composition predictions is heavily reliant on a pre-processing step to remove insignificant spectral variation. This is crucial in biofluid analysis. Widespread adoption of diagnostics…

Signal Processing · Electrical Eng. & Systems 2019-04-05 Emily E Storey , Amr S. Helmy

Label-free detecting multiple analytes in a high-throughput fashion has been one of the long-sought goals in biosensing applications. Yet, for all-optical approaches, interfacing state-of-the-art label-free techniques with microfluidics…

Quantitative Methods · Quantitative Biology 2023-08-14 Alexia Stollmann , Jose Garcia-Guirado , Jae-Sang Hong , Hyungsoon Im , Hakho Lee , Jaime Ortega Arroyo , Romain Quidant

Raman spectroscopy is a label-free, chemically specific optical technique which provides detailed information about the chemical composition and structure of the excited analyte. Because of this, there is growing research interest in…

Time-resolved spectral techniques play an important analysis tool in many contexts, from physical chemistry to biomedicine. Customarily, the label-free detection of analytes is manually performed by experts through the aid of classic…

Machine Learning · Computer Science 2020-11-17 Stefano Rini , Hirotsugu Hiramatsu

Droplet microfluidics offers a versatile platform for analyzing liquid samples. Despite its potential, there is a lack of techniques that allow to reliably probe individual circulating droplets. The prospect of combining droplet…

Quantitative Methods · Quantitative Biology 2024-03-08 Marc Sulliger , Jaime Ortega Arroyo , Romain Quidant

In chemical processing and bioprocessing, conventional online sensors are limited to measure only basic process variables like pressure and temperature, pH, dissolved O and CO$_2$ and viable cell density (VCD). The concentration of other…

Quantitative Methods · Quantitative Biology 2020-05-07 Semion Rozov

Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the possible photodamage risk hinder further clinical applications. To reduce these adverse effects, we constructed a new platform of…

Optics · Physics 2022-10-26 Geng Wang , Stephen A. Boppart , Haohua Tu

We present a wide-field multi-pass implementation of label-free imaging flow cytometry. Our technique is shown for high-speed flow imaging of ensembles of human red blood cells with up to four passes, demonstrating x4 enhancement in…

Ultrasound vascular imaging is limited by acoustic diffraction, restricting visualization of microvessels essential for understanding organ function and disease. Label-free super-resolution methods exploiting endogenous red blood cells have…

Continuous ambulatory monitoring of peripheral vascular perfusion could enable earlier detection of vascular dysfunction in individuals with diabetes mellitus and more timely management of cardiovascular disease. Clinical imaging modalities…

Deep learning classifiers provide the most accurate means of automatically diagnosing diabetic retinopathy (DR) based on optical coherence tomography (OCT) and its angiography (OCTA). The power of these models is attributable in part to the…

Image and Video Processing · Electrical Eng. & Systems 2023-06-28 Pengxiao Zang , Tristan T. Hormel , Jie Wang , Yukun Guo , Steven T. Bailey , Christina J. Flaxel , David Huang , Thomas S. Hwang , Yali Jia

Segmentation and measurement of cardiac chambers is critical in cardiac ultrasound but is laborious and poorly reproducible. Neural networks can assist, but supervised approaches require the same laborious manual annotations. We built a…

Image and Video Processing · Electrical Eng. & Systems 2026-02-24 Danielle L. Ferreira , Connor Lau , Zaynaf Salaymang , Rima Arnaout

Confocal Raman microscopy offers a particular pathway for monitoring chemical "fingerprints" of intracellular components like the cell membrane, organelles, and nucleus. Nevertheless, conventional Raman acquisitions of fixed or randomly…

Label-free biosensors are important tools for clinical diagnostics and for studying biology at the single molecule level. The development of optical label-free sensors has allowed extreme sensitivity, but can expose the biological sample to…

Confocal Raman microscopy, a highly specific and label-free technique for the microscale study of thick samples, often presents difficulties due to weak Raman signals. Inhomogeneous samples introduce wavefront aberrations that further…

We demonstrate stimulated Raman gain using a broadband LED Stokes source to measure vibrational spectra of aqueous glucose solutions. This versatile and cost-effective method increases Raman signal for a variety of applications. We measured…

Applied Physics · Physics 2018-09-14 Peter Bullen , Ioannis Kymissis , Adler Perotte

Blood analysis is an important diagnostic tool, as it provides a wealth of information about the patient health. Raman spectroscopy is a promising tool in blood analysis, but widespread clinical application is limited by its low signal…

Hydrogen detection is of great importance in chemical and energy industries. Optical fiber hydrogen sensors show flexibility and compactness and have the potential for distributed analysis. However, traditional fiber sensors encounter a…

Optics · Physics 2019-05-16 F. Yang , Y. Zhao , Y. Qi , Y. Z. Tan , H. L. Ho , W. Jin

The emergence of label-free microscopy techniques has significantly improved our ability to precisely characterize biochemical targets, enabling non-invasive visualization of cellular organelles and tissue organization. Each label-free…

Optics · Physics 2024-05-08 Lang Wang , Maxine Xii , Ali Pezeshki , Randy Bartels

Tuberculosis (TB) is a contagious disease that causes 1.5 million deaths per year globally. Early diagnosis of TB patients is critical to control its spread. However, standard TB diagnostic tests such as sputum culture take days to weeks to…

Medical Physics · Physics 2022-08-26 Ubaid Ullah , Zarfishan Tahir , Obaidullah Qazi , Shaper Mirza , M. Imran Cheema
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