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Following recent advancements in multimode fiber (MMF), miniaturization of imaging endoscopes has proven crucial for minimally invasive surgery in vivo. Recent progress enabled by super-resolution imaging methods with a data-driven deep…

Optics · Physics 2023-07-12 Chuncheng Zhang , Tingting Liu , Zhihua Xie , Yu Wang , Tong Liu , Qian Chen , Xiubao Sui

Lensless fiber endomicroscope is an emerging tool for in-vivo microscopic imaging, where quantitative phase imaging (QPI) can be utilized as a label-free method to enhance image contrast. However, existing single-shot phase reconstruction…

Confocal laser-scanning microscopy (CLSM) is one of the most popular optical architectures for fluorescence imaging. In CLSM, a focused laser beam excites the fluorescence emission from a specific specimen position. Some actuators scan the…

Image and Video Processing · Electrical Eng. & Systems 2023-05-10 Alessandro Zunino , Marco Castello , Giuseppe Vicidomini

The thin and flexible nature of optical fibres often makes them the ideal technology to view biological processes in-vivo, but current microendoscopic approaches are limited in spatial resolution. Here, we demonstrate a new route to high…

Light rays incident on a transparent object of uniform refractive index undergo deflections, which uniquely characterize the surface geometry of the object. Associated with each point on the surface is a deflection map (or spectrum) which…

Computer Vision and Pattern Recognition · Computer Science 2015-07-15 Prasad Sudhakar , Laurent Jacques , Xavier Dubois , Philippe Antoine , Luc Joannes

Scanning fluorescence correlation spectroscopy (SFCS) with a scan path perpendicular to the membrane plane was introduced to measure diffusion and interactions of fluorescent components in free standing biomembranes. Using a confocal laser…

Biomolecules · Quantitative Biology 2018-06-04 Paul Müller , Petra Schwille , Thomas Weidemann

This paper considers a compressive multi-spectral light field camera model that utilizes a one-hot spectralcoded mask and a microlens array to capture spatial, angular, and spectral information using a single monochrome sensor. We propose a…

Computer Vision and Pattern Recognition · Computer Science 2025-03-18 Wen Cao , Ehsan Miandji , Jonas Unger

We develop a lensless compressive imaging architecture, which consists of an aperture assembly and a single sensor, without using any lens. An anytime algorithm is proposed to reconstruct images from the compressive measurements; the…

Computer Vision and Pattern Recognition · Computer Science 2015-08-17 Xin Yuan , Hong Jiang , Gang Huang , Paul Wilford

Optical endoscopy plays a crucial role in minimally invasive medical diagnostics and therapeutic procedures. Nonetheless, state-of-the-art endoscopic zoom objectives are bulky, requiring multi-element lens actuation, which can limit…

Intra-operative measurements of tissue shape and multi/ hyperspectral information have the potential to provide surgical guidance and decision making support. We report an optical probe based system to combine sparse hyperspectral…

Computer Vision and Pattern Recognition · Computer Science 2017-06-22 Jianyu Lin , Neil T. Clancy , Yang Hu , Ji Qi , Taran Tatla , Danail Stoyanov , Lena Maier-Hein , Daniel S. Elson

The flexible endoscope is a minimally invasive tool in clinical settings, but most of them rely on exogenous staining for diagnosis to provide qualitative information. Here, we demonstrated a flexible endoscopic microscopy (FEM) with…

Fluorescence microscopy has been a significant tool to observe long-term imaging of embryos (in vivo) growth over time. However, cumulative exposure is phototoxic to such sensitive live samples. While techniques like light-sheet…

Image and Video Processing · Electrical Eng. & Systems 2022-01-05 Varun Mannam , Jacob Brandt , Cody J. Smith , Scott Howard

Confocal laser scanning microscopy (CLSM) stands out as one of the most widely used microscopy techniques, thanks to its three-dimensional imaging capability and its sub-diffraction spatial resolution, achieved through the closure of a…

Fiber-based endoscopes utilizing multi-core fiber (MCF) bundles offer the capability to image deep within the human body, making them well-suited for imaging applications in minimally invasive surgery or diagnosis. However, the optical…

We developed a Bessel light sheet fluorescence microscopy (LSFM) system to enable high-speed, wide-field intra-vital imaging of zebrafish and other thick biological samples. This system uses air objectives for the convenient mounting of…

Optics · Physics 2023-11-07 Chuhui Wang , Jiaju Chen , Cuiyi Peng , Zhenglin Chen , Dongmei Yu , Peiwu Qin

Compressed sensing (CS) is a valuable technique for reconstructing measurements in numerous domains. CS has not yet gained widespread adoption in scanning tunneling microscopy (STM), despite potentially offering the advantages of lower…

Mesoscale and Nanoscale Physics · Physics 2022-02-09 Brian E. Lerner , Anayeli Flores-Garibay , Benjamin J. Lawrie , Petro Maksymovych

Lensless imaging stands out as a promising alternative to conventional lens-based systems, particularly in scenarios demanding ultracompact form factors and cost-effective architectures. However, such systems are fundamentally governed by…

Image and Video Processing · Electrical Eng. & Systems 2025-05-06 Jiesong Bai , Yuhao Yin , Yihang Dong , Xiaofeng Zhang , Chi-Man Pun , Xuhang Chen

We present a physics-informed deep learning framework to address common limitations in Confocal Laser Scanning Microscopy (CLSM), such as diffraction limited resolution, noise, and undersampling due to low laser power conditions. The…

Materials Science · Physics 2025-01-27 Zaheer Ahmad , Junaid Shabeer , Usman Saleem , Tahir Qadeer , Abdul Sami , Zahira El Khalidi , Saad Mehmood

Fast and sensitive detector arrays enable image scanning microscopy (ISM), overcoming the trade-off between spatial resolution and signal-to-noise ratio (SNR) typical of confocal microscopy. However, current ISM approaches cannot provide…

We demonstrate pixelation-free real-time widefield endoscopic imaging through an aperiodic multicore fiber (MCF) without any distal opto-mechanical elements or proximal scanners. Exploiting the memory effect in MCFs the images in our system…