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Fluorescence microscopy images usually show severe anisotropy in axial versus lateral resolution. This hampers downstream processing, i.e. the automatic extraction of quantitative biological data. While deconvolution methods and other…

Computer Vision and Pattern Recognition · Computer Science 2017-04-06 Martin Weigert , Loic Royer , Florian Jug , Gene Myers

Rendering an accurate image of an isosurface in a volumetric field typically requires large numbers of data samples. Reducing the number of required samples lies at the core of research in volume rendering. With the advent of deep learning…

Graphics · Computer Science 2022-05-31 Sebastian Weiss , Mengyu Chu , Nils Thuerey , Rüdiger Westermann

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

Image and Video Processing · Electrical Eng. & Systems 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard

We present a deep learning driven computational approach to overcome the limitations of self-interference digital holography that imposed by inferior axial imaging performances. We demonstrate a 3D deep neural network model can…

We demonstrate that a deep neural network can significantly improve optical microscopy, enhancing its spatial resolution over a large field-of-view and depth-of-field. After its training, the only input to this network is an image acquired…

Machine Learning · Computer Science 2017-11-21 Yair Rivenson , Zoltan Gorocs , Harun Gunaydin , Yibo Zhang , Hongda Wang , Aydogan Ozcan

Confocal microscopy is the standard approach for obtaining volumetric images of a sample with high axial and lateral resolution, especially when dealing with scattering samples. Unfortunately, a confocal microscope is quite expensive…

The most sophisticated existing methods to generate 3D isotropic super-resolution (SR) from non-isotropic electron microscopy (EM) are based on learned dictionaries. Unfortunately, none of the existing methods generate practically…

Computer Vision and Pattern Recognition · Computer Science 2017-06-13 Larissa Heinrich , John A. Bogovic , Stephan Saalfeld

Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D…

Computer Vision and Pattern Recognition · Computer Science 2019-11-05 Yichen Wu , Yair Rivenson , Hongda Wang , Yilin Luo , Eyal Ben-David , Laurent A. Bentolila , Christian Pritz , Aydogan Ozcan

Although high resolution isotropic 3D medical images are desired in clinical practice, their acquisition is not always feasible. Instead, lower resolution images are upsampled to higher resolution using conventional interpolation methods.…

Image and Video Processing · Electrical Eng. & Systems 2020-10-27 Jörg Sander , Bob D. de Vos , Ivana Išgum

Three-dimensional electron microscopy (3DEM) is an essential technique to investigate volumetric tissue ultra-structure. Due to technical limitations and high imaging costs, samples are often imaged anisotropically, where resolution in the…

Image and Video Processing · Electrical Eng. & Systems 2023-09-20 Mohammad Khateri , Morteza Ghahremani , Alejandra Sierra , Jussi Tohka

Performing super-resolution of a depth image using the guidance from an RGB image is a problem that concerns several fields, such as robotics, medical imaging, and remote sensing. While deep learning methods have achieved good results in…

Computer Vision and Pattern Recognition · Computer Science 2023-03-29 Nando Metzger , Rodrigo Caye Daudt , Konrad Schindler

Analysis and visualization of 3D microscopy images pose challenges due to anisotropic axial resolution, demanding volumetric super-resolution along the axial direction. While training a learning-based 3D super-resolution model seems to be a…

Image and Video Processing · Electrical Eng. & Systems 2024-08-19 Kyungryun Lee , Won-Ki Jeong

Imaging through scattering is a pervasive and difficult problem in many biological applications. The high background and the exponentially attenuated target signals due to scattering fundamentally limits the imaging depth of fluorescence…

Signal Processing · Electrical Eng. & Systems 2023-12-11 Jeffrey Alido , Joseph Greene , Yujia Xue , Guorong Hu , Yunzhe Li , Mitchell Gilmore , Kevin J. Monk , Brett T. DiBenedictis , Ian G. Davison , Lei Tian

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, and complexity, and thus cannot fulfill the emerging need of…

Optics · Physics 2022-09-09 Yujia Xue , Qianwan Yang , Guorong Hu , Kehan Guo , Lei Tian

Volumetric imaging of samples using fluorescence microscopy plays an important role in various fields including physical, medical and life sciences. Here we report a deep learning-based volumetric image inference framework that uses 2D…

Optics · Physics 2021-03-24 Luzhe Huang , Yilin Luo , Yair Rivenson , Aydogan Ozcan

High-resolution fMRI provides a window into the brain's mesoscale organization. Yet, higher spatial resolution increases scan times, to compensate for the low signal and contrast-to-noise ratio. This work introduces a deep learning-based 3D…

Image and Video Processing · Electrical Eng. & Systems 2024-03-20 Hongwei Bran Li , Matthew S. Rosen , Shahin Nasr , Juan Eugenio Iglesias

This chapter provides an overview of deep learning techniques for improving the spatial resolution of MRI, ranging from convolutional neural networks, generative adversarial networks, to more advanced models including transformers,…

Computer Vision and Pattern Recognition · Computer Science 2024-10-23 Ziyu Li , Zihan Li , Haoxiang Li , Qiuyun Fan , Karla L. Miller , Wenchuan Wu , Akshay S. Chaudhari , Qiyuan Tian

In the field of fusing multi-spectral and panchromatic images (Pan-sharpening), the impressive effectiveness of deep neural networks has been recently employed to overcome the drawbacks of traditional linear models and boost the fusing…

Computer Vision and Pattern Recognition · Computer Science 2017-11-22 Yancong Wei , Qiangqiang Yuan , Huanfeng Shen , Liangpei Zhang

The use of fluorescent molecules to create long sequences of low-density, diffraction-limited images enables highly-precise molecule localization. However, this methodology requires lengthy imaging times, which limits the ability to view…

Image and Video Processing · Electrical Eng. & Systems 2024-03-26 Yair Ben Sahel , Yonina C. Eldar

Super-resolution is an innovative technique that upscales the resolution of an image or a video and thus enables us to reconstruct high-fidelity images from low-resolution data. This study performs super-resolution analysis on turbulent…

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