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Fourier transform infrared (FTIR) spectrometers have been the dominant technology in the field of mid-infrared (MIR)spectroscopy for decades. Supercontinuum laser sources operating in the MIR spectral region now offer the potential to…
Mid-infrared spectroscopy is often used to identify material. Thousands of spectral points are measured in a time-consuming process using expensive table-top instrument. However, material identification is a sparse problem, which in theory…
Fluorescence imaging is the most widely used method for unveiling the molecular composition of biological specimens. However, the weak optical emission of fluorescent probes and the tradeoff between imaging speed and sensitivity is…
Absorption microscopy is a powerful technique, enabling the detection of single non- fluorescent molecules at room temperature. So far, the molecular absorption has been probed optically via the attenuation of a probing laser. The…
Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and…
Fluorescent nanothermometry has revealed pronounced intracellular temperature heterogeneity, establishing the field of single-cell thermal biology. However, these observations have sparked a controversy known as the "10^5 gap issue",…
Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…
We present an analytical description and an experimental realization of interscale mixing microscopy, a diffraction-based imaging technique that is capable of detecting wavelength/10 objects in far-field measurements with both coherent and…
Intraoperative optical imaging is essential for surgical precision and patient safety, but current systems present anatomical and fluorescence information separately, causing delays and increasing cognitive load. A unified system for…
Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…
Label-free imaging of rapidly moving, sub-diffraction sized structures has important applications in both biology and material science, as it removes the limitations associated with fluorescence tagging. However, unlabeled nanoscale…
Fourier-transform infrared spectroscopy (FTIR) has matured into a versatile technique with relevance for environmental monitoring, pharmaceutical research, and food safety applications. However, compared to other spectroscopic methods, it…
Structured illumination microscopy (SIM) has attained high spatiotemporal delineation of subcellular architecture, yet offers limited insight into chemical composition. We develop Chem-SIM, a structured-illumination fluorescence detected…
Mid-InfraRed spectroscopy is a promising label-free technique that can offer insights into morphological and pathological alterations in biological tissues at the molecular level. Owing to the development of the Fourier Transform InfraRed…
Infrared (IR) spectroscopy has emerged as a pivotal tool in biomedical diagnostics, offering label-free spectral biomarkers for the detection of numerous diseases, particularly in the fingerprint region. However, the lack of rapid and…
The mid-infrared (mid-IR) spectral range is a part of the electromagnetic spectrum in which most of the molecules have vibrational and rotational resonances. Ge-based photonic integrated circuits in this wavelength range have thus seen a…
Mid-infrared (mid-IR) spectroscopy is a crucial workhorse for a plethora of analytical applications and is suitable for diverse materials, including gases, polymers or biological tissue. However, this technologically significant wavelength…
We present a system for molecular spectroscopy using a broadband mid-infrared laser with near infrared detection. Difference frequency generation of a Yb:fiber femtosecond laser produced a mid-infrared (MIR) source tunable from 2100-3700…
Spectral measurements in the infrared (IR) optical range provide unique fingerprints of materials which are useful for material analysis, environmental sensing, and health diagnostics. Current IR spectroscopy techniques require the use of…
Accurate direct measurements of far-field thermal infrared emission become increasingly important because conventional methods, relying on indirect assessments, such as reflectance/transmittance, are inaccurate or even unfeasible to…