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A common task in cryo-electron microscopy (cryo-EM) data processing is to compare three-dimensional density maps of macromolecules. In this paper, we propose an algorithm for aligning three-dimensional density maps that exploits common…

Image and Video Processing · Electrical Eng. & Systems 2023-02-27 Yael Harpaz , Yoel Shkolnisky

We present a novel method for Ankylography: three-dimensional structure reconstruction from a single shot diffraction intensity pattern. Our approach allows reconstruction of objects containing many more details than was ever demonstrated,…

Optics · Physics 2012-03-22 Eliyahu Osherovich , Oren Cohen , Yonina C. Eldar , Mordechai Segev

This work proposes novel hyperparameter-free losses for single view 3D reconstruction with morphable models (3DMM). We dispense with the hyperparameters used in other works by exploiting geometry, so that the shape of the object and the…

Computer Vision and Pattern Recognition · Computer Science 2019-08-28 Eduard Ramon , Guillermo Ruiz , Thomas Batard , Xavier Giró-i-Nieto

The core theme of X-ray crystallography is reconstructing the electron density distribution of crystals under the constraints of observed diffraction data. Nevertheless, the reconstruction of electron density distribution by straightforward…

Materials Science · Physics 2015-07-14 Hui Li , Meng He , Ze Zhang

Direct detector device (DDD) cameras have revolutionized single particle electron cryomicroscopy (cryo-EM). In addition to an improved camera detective quantum efficiency, acquisition of DDD movies allows for correction of movement of the…

Quantitative Methods · Quantitative Biology 2015-11-23 John L. Rubinstein , Marcus A. Brubaker

Single-particle cryo-electron microscopy (cryo-EM) has become one of the mainstream structural biology techniques because of its ability to determine high-resolution structures of dynamic bio-molecules. However, cryo-EM data acquisition…

Machine Learning · Computer Science 2022-04-18 Quanfu Fan , Yilai Li , Yuguang Yao , John Cohn , Sijia Liu , Seychelle M. Vos , Michael A. Cianfrocco

In the past decade, deep conditional generative models have revolutionized the generation of realistic images, extending their application from entertainment to scientific domains. Single-particle cryo-electron microscopy (cryo-EM) is…

Computer Vision and Pattern Recognition · Computer Science 2024-10-01 Jiakai Zhang , Qihe Chen , Yan Zeng , Wenyuan Gao , Xuming He , Zhijie Liu , Jingyi Yu

We propose a novel dynamic image reconstruction method from PET listmode data that could be particularly suited to tracking single or small numbers of cells. In contrast to conventional PET reconstruction our method combines the information…

Computer Vision and Pattern Recognition · Computer Science 2019-11-25 Bernhard Schmitzer , Klaus P. Schäfers , Benedikt Wirth

Lensless in-line holography is a simple, portable, and cost-effective method of imaging especially for the biomedical microscopy applications. We propose a multiplicative gradient descent optimization based method to obtain multi-depth…

Image and Video Processing · Electrical Eng. & Systems 2020-04-20 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

Cryo-electron microscopy provides 2-D projection images of the 3-D electron scattering intensity of many instances of the particle under study (e.g., a virus). Both symmetry (rotational point groups) and heterogeneity are important aspects…

Applications · Statistics 2019-05-17 Nan Xu , Peter C Doerschuk

Single-pixel imaging (SPI) is significant for applications constrained by transmission bandwidth or lighting band, where 3D SPI can be further realized through capturing signals carrying depth. Sampling strategy and reconstruction algorithm…

Image and Video Processing · Electrical Eng. & Systems 2025-04-01 Xinyue Ma , Chenxing Wang

We study end-to-end learning strategies for 3D shape inference from images, in particular from a single image. Several approaches in this direction have been investigated that explore different shape representations and suitable learning…

Computer Vision and Pattern Recognition · Computer Science 2019-08-21 Roman Klokov , Jakob Verbeek , Edmond Boyer

Single-pixel imaging (SPI) is a novel imaging technique whose working principle is based on the compressive sensing (CS) theory. In SPI, data is obtained through a series of compressive measurements and the corresponding image is…

Image and Video Processing · Electrical Eng. & Systems 2022-07-15 Stephen L. H. Lau , Edwin K. P. Chong

Reconstructing the 3D shape of an object from a single RGB image is a long-standing and highly challenging problem in computer vision. In this paper, we propose a novel method for single-image 3D reconstruction which generates a sparse…

Computer Vision and Pattern Recognition · Computer Science 2023-02-24 Luke Melas-Kyriazi , Christian Rupprecht , Andrea Vedaldi

Cryo-electron microscopy (cryo-EM) emerges as a pivotal technology for determining the architecture of cells, viruses, and protein assemblies at near-atomic resolution. Traditional particle picking, a key step in cryo-EM, struggles with…

Biomolecules · Quantitative Biology 2024-04-17 Chentianye Xu , Xueying Zhan , Min Xu

In this paper, we present a novel Bayesian approach for estimating spectral and range profiles from single-photon Lidar waveforms associated with single surfaces in the photon-limited regime. In contrast to classical multispectral Lidar…

Image and Video Processing · Electrical Eng. & Systems 2019-12-13 Quentin Legros , Sylvain Meignen , Stephen McLaughlin , Yoann Altmann

One of the difficulties in 3D reconstruction of molecules from images in single particle Cryo-Electron Microscopy (Cryo-EM), in addition to high levels of noise and unknown image orientations, is heterogeneity in samples: in many cases, the…

Computer Vision and Pattern Recognition · Computer Science 2016-07-13 Roy R. Lederman , Amit Singer

With the rapid development of computer graphics and vision, several three-dimensional (3D) reconstruction techniques have been proposed and used to obtain the 3D representation of objects in the form of point cloud models, mesh models, and…

Computer Vision and Pattern Recognition · Computer Science 2021-12-02 Rui Wang

This paper focuses on the problem of 3D human reconstruction from 2D evidence. Although this is an inherently ambiguous problem, the majority of recent works avoid the uncertainty modeling and typically regress a single estimate for a given…

Computer Vision and Pattern Recognition · Computer Science 2021-08-27 Nikos Kolotouros , Georgios Pavlakos , Dinesh Jayaraman , Kostas Daniilidis

An efficient computational approach for optimal reconstruction of binary-type images suitable for models in various applications including biomedical imaging is developed and validated. The methodology includes derivative-free optimization…

Optimization and Control · Mathematics 2022-09-27 Paul R. Arbic , Vladislav Bukshtynov