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Over the last decade, single-molecule optical microscopy has become the gold-standard approach to decipher complex molecular processes in cellular environments. [1-3] Single-molecule fluorescence microscopy has several advantages such as…

Biological Physics · Physics 2017-11-15 Edakkattuparambil Sidharth Shibu , Nadezda Varkentina , Laurent Cognet , Brahim Lounis

In biological microscopy, light scattering represents the main limitation to image at depth. Recently, a set of wavefront shaping techniques has been developed in order to manipulate coherent light in strongly disordered materials. The…

Optics · Physics 2021-01-07 Antoine Boniface , Jonathan Dong , Sylvain Gigan

Interferometric scattering microscopy is a powerful technique that enables various applications, such as mass photometry and particle tracking. Here we present a numerical toolbox to simulate images obtained in interferometric scattering,…

Single-mode optical nanofibres are a central component of a broad range of applications and emerging technologies. Their fabrication has been extensively studied over the past decade, but imaging of the final sub-micrometre products has…

Reflection phase imaging provides label-free, high-resolution characterization of biological samples, typically using interferometric-based techniques. Here, we investigate reflection phase microscopy from intensity-only measurements under…

Quantitative Methods · Quantitative Biology 2019-12-18 Alex Matlock , Anne Sentenac , Patrick C. Chaumet , Ji Yi , Lei Tian

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester

Thick biological tissues give rise to not only the scattering of incoming light waves, but also aberrations of the remaining unscattered waves. Due to the inability of existing optical imaging methodologies to overcome both of these…

We investigate the interaction of tightly focused light with the surface-plasmon-polariton resonances of metal nanospheres. In particular, we compute the scattering and absorption ratios as well as transmission and reflection coefficients.…

Optics · Physics 2009-05-13 Nassiredin Mojarad , Gert Zumofen , Vahid Sandoghdar , Mario Agio

Single molecule tracking in live cells is the ultimate tool to study subcellular protein dynamics, but it is often limited by the probe size and photostability. Due to these issues, long-term tracking of proteins in confined and crowded…

Metasurfaces are paving the way to improve traditional optical components by integrating multiple functionalities into one optically flat metasurface design. We demonstrate the implementation of a multifunctional gap surface plasmon-based…

Mesoscale and Nanoscale Physics · Physics 2024-12-11 Sergejs Boroviks , Rucha A. Deshpande , N. Asger Mortensen , Sergey I. Bozhevolnyi

Label-free imaging of rapidly moving, sub-diffraction sized structures has important applications in both biology and material science, as it removes the limitations associated with fluorescence tagging. However, unlabeled nanoscale…

Dark-field X-ray imaging visualizes structural inhomogeneities through small-angle scattering, but existing directional methods are confined to the micrometer scale. While recent advances have extended dark-field capabilities to nanoscale…

An ideal solid-state supermirror (SM) neutron polarizer assumes total reflection of neutrons from the SM coating for one spin-component and total absorption for the other, thus providing a perfectly polarized neutron beam at the exit.…

Instrumentation and Detectors · Physics 2016-10-12 A. K. Petukhov , V. V. Nesvizhevsky , T. Bigault , P. Courtois , D. Jullien , T. Soldner

Detection and characterization of individual nano-scale particles, virions, and pathogens are of paramount importance to human health, homeland security, diagnostic and environmental monitoring[1]. There is a strong demand for…

Optics · Physics 2011-07-06 Lina He , Sahin Kaya Ozdemir , Jiangang Zhu , Woosung Kim , Lan Yang

Label free tracking of small bio-particles such as proteins or viruses is of great utility in the study of biological processes, however such experiments are frequently hindered by weak signal strengths and a susceptibility to scattering…

Optics · Physics 2021-08-03 Joel Berk , Carl Paterson , Matthew R. Foreman

We describe a method that uses total internal reflection at the water-air interface inside a large, transparent tank, to measure the interface's deflections. Using this configuration, we obtain an optical set-up where the liquid surface…

Fluid Dynamics · Physics 2021-10-26 Utkarsh Jain , Anaïs Gauthier , Devaraj van der Meer

We introduce a point-like scanning single-photon source that operates at room temperature and offers an exceptional photostability (no blinking, no bleaching). This is obtained by grafting in a controlled way a diamond nanocrystal (size…

Optics · Physics 2009-10-25 A. Cuche , A. Drezet , Y. Sonnefraud , O. Faklaris , F. Treussart , J. -F. Roch , S. Huant

A novel finite-element method for calculating the illumination-dependence of absorption in three-dimensional nanostructures is presented based on the RF module of the COMSOL software package. This method is capable of numerically…

Optics · Physics 2015-05-28 Mária Csete , Áron Sipos , Faraz Najafi , Xiaolong Hu , Karl K. Berggren

A neutron optical transport system is proposed which comprises nested short elliptical mirrors located halfway between two common focal points M and M'. It images cold neutrons from a diverging beam or a source with finite size at M by…

Instrumentation and Detectors · Physics 2016-11-23 Oliver Zimmer

We experimentally show an all-optical multipolar decomposition of the lowest-order Eigenmodes of a single gold nanoprism using azimuthally and radially polarized cylindrical vector beams. By scanning the particle through these tailored…

Optics · Physics 2015-03-10 Thomas Bauer , Sergej Orlov , Gerd Leuchs , Peter Banzer