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Scanning microscopy systems, such as confocal and multiphoton microscopy, are powerful imaging tools for probing deep into biological tissue. However, scanning systems have an inherent trade-off between acquisition time, field of view,…

Image and Video Processing · Electrical Eng. & Systems 2025-03-25 Cassandra Tong Ye , Jiashu Han , Kunzan Liu , Anastasios Angelopoulos , Linda Griffith , Kristina Monakhova , Sixian You

With super-resolution optical microscopy, it is now possible to observe molecular interactions in living cells. The obtained images have a very high spatial precision but their overall quality can vary a lot depending on the structure of…

Computer Vision and Pattern Recognition · Computer Science 2018-03-30 Louis-Émile Robitaille , Audrey Durand , Marc-André Gardner , Christian Gagné , Paul De Koninck , Flavie Lavoie-Cardinal

This work considers supervised learning to count from images and their corresponding point annotations. Where density-based counting methods typically use the point annotations only to create Gaussian-density maps, which act as the…

Computer Vision and Pattern Recognition · Computer Science 2023-06-09 Zenglin Shi , Pascal Mettes , Cees G. M. Snoek

Single cell segmentation is critical and challenging in live cell imaging data analysis. Traditional image processing methods and tools require time-consuming and labor-intensive efforts of manually fine-tuning parameters. Slight variations…

Quantitative Methods · Quantitative Biology 2019-04-24 Weikang Wang , David A. Taft , Yi-Jiun Chen , Jingyu Zhang , Callen T. Wallace , Min Xu , Simon C. Watkins , Jianhua Xing

Pathology deals with the practice of discovering the reasons for disease by analyzing the body samples. The most used way in this field, is to use histology which is basically studying and viewing microscopic structures of cell and tissues.…

Image and Video Processing · Electrical Eng. & Systems 2021-11-08 Virender Ranga , Shivam Gupta , Priyansh Agrawal , Jyoti Meena

State-of-the-art object detection and segmentation methods for microscopy images rely on supervised machine learning, which requires laborious manual annotation of training data. Here we present a self-supervised method based on time arrow…

Computer Vision and Pattern Recognition · Computer Science 2023-07-27 Benjamin Gallusser , Max Stieber , Martin Weigert

Automated detection of cervical cancer cells or cell clumps has the potential to significantly reduce error rate and increase productivity in cervical cancer screening. However, most traditional methods rely on the success of accurate cell…

Computer Vision and Pattern Recognition · Computer Science 2019-12-24 Yixiong Liang , Zhihong Tang , Meng Yan , Jialin Chen , Qing Liu , Yao Xiang

Autofocus (AF) methods are extensively used in biomicroscopy, for example to acquire timelapses, where the imaged objects tend to drift out of focus. AD algorithms determine an optimal distance by which to move the sample back into the…

Image and Video Processing · Electrical Eng. & Systems 2021-01-14 Adrian Shajkofci , Michael Liebling

This paper aims to count arbitrary objects in images. The leading counting approaches start from point annotations per object from which they construct density maps. Then, their training objective transforms input images to density maps…

Computer Vision and Pattern Recognition · Computer Science 2019-08-07 Zenglin Shi , Pascal Mettes , Cees G. M. Snoek

Segmentation of objects in microscopy images is required for many biomedical applications. We introduce object-centric embeddings (OCEs), which embed image patches such that the spatial offsets between patches cropped from the same object…

Machine Learning · Computer Science 2023-10-13 Steffen Wolf , Manan Lalit , Henry Westmacott , Katie McDole , Jan Funke

Image analysis is a key tool for describing the detailed mechanisms of folliculogenesis, such as evaluating the quantity of mouse Primordial ovarian Follicles (PMF) in the ovarian reserve. The development of high-resolution virtual slide…

We developed a transparent computational large-scale imaging-based framework that can distinguish between normal and metastasizing human cells. The method relies on fluorescence microscopy images showing the spatial organization of actin…

Image and Video Processing · Electrical Eng. & Systems 2024-09-04 Michail Mamalakis , Sarah C. Macfarlane , Scott V. Notley , Annica K. B Gad , George Panoutsos

Advances in artificial intelligence (AI) show great potential in revealing underlying information from phonon microscopy (high-frequency ultrasound) data to identify cancerous cells. However, this technology suffers from the 'batch effect'…

Quantitative Methods · Quantitative Biology 2024-03-28 Yijie Zheng , Rafael Fuentes-Dominguez , Matt Clark , George S. D. Gordon , Fernando Perez-Cota

Machine learning and artificial intelligence are fast-growing fields of research in which data is used to train algorithms, learn patterns, and make predictions. This approach helps to solve seemingly intricate problems with significant…

Computer Vision and Pattern Recognition · Computer Science 2025-12-23 Shereiff Garrett , Abhinav Adhikari , Sarina Gautam , DaShawn Marquis Morris , Chandra Mani Adhikari

Automated recognition and classification of bacteria species from microscopic images have significant importance in clinical microbiology. Bacteria classification is usually carried out manually by biologists using different shapes and…

Quantitative Methods · Quantitative Biology 2019-12-19 Muhammed Talo

Training deep neural networks from few examples is a highly challenging and key problem for many computer vision tasks. In this context, we are targeting knowledge transfer from a set with abundant data to other sets with few available…

Computer Vision and Pattern Recognition · Computer Science 2019-03-13 Yann Lifchitz , Yannis Avrithis , Sylvaine Picard , Andrei Bursuc

Detecting and segmenting object instances is a common task in biomedical applications. Examples range from detecting lesions on functional magnetic resonance images, to the detection of tumours in histopathological images and extracting…

Computer Vision and Pattern Recognition · Computer Science 2021-04-12 Tim Prangemeier , Christoph Reich , Heinz Koeppl

While most previous automation-assisted reading methods can improve efficiency, their performance often relies on the success of accurate cell segmentation and hand-craft feature extraction. This paper presents an efficient and totally…

Computer Vision and Pattern Recognition · Computer Science 2019-12-17 Yao Xiang , Wanxin Sun , Changli Pan , Meng Yan , Zhihua Yin , Yixiong Liang

Automated skin lesion classification using deep learning has shown remarkable accuracy, yet clinical adoption remains limited due to the "black box" nature of these models. We present MelanomaNet, an explainable deep learning system for…

Computer Vision and Pattern Recognition · Computer Science 2025-12-11 Sukhrobbek Ilyosbekov

Label-free imaging has gained broad interest because of its potential to omit elaborate staining procedures which is especially relevant for in vivo use. Label-free multiphoton microscopy (MPM), for instance, exploits two-photon excitation…