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The future landscape of modern farming and plant breeding is rapidly changing due to the complex needs of our society. The explosion of collectable data has started a revolution in agriculture to the point where innovation must occur. To a…

Computer Vision and Pattern Recognition · Computer Science 2020-10-26 Saeed Khaki , Hieu Pham , Ye Han , Wade Kent , Lizhi Wang

Object counting and localization are key steps for quantitative analysis in large-scale microscopy applications. This procedure becomes challenging when target objects are overlapping, are densely clustered, and/or present fuzzy boundaries.…

Computer Vision and Pattern Recognition · Computer Science 2022-03-30 Shijie Li , Thomas Ach , Guido Gerig

Counting objects in digital images is a process that should be replaced by machines. This tedious task is time consuming and prone to errors due to fatigue of human annotators. The goal is to have a system that takes as input an image and…

Computer Vision and Pattern Recognition · Computer Science 2017-07-25 Joseph Paul Cohen , Genevieve Boucher , Craig A. Glastonbury , Henry Z. Lo , Yoshua Bengio

Learning to count is a learning strategy that has been recently proposed in the literature for dealing with problems where estimating the number of object instances in a scene is the final objective. In this framework, the task of learning…

Computer Vision and Pattern Recognition · Computer Science 2015-06-01 Santi Seguí , Oriol Pujol , Jordi Vitrià

Volumetric cell segmentation in fluorescence microscopy images is important to study a wide variety of cellular processes. Applications range from the analysis of cancer cells to behavioral studies of cells in the embryonic stage. Like in…

Image and Video Processing · Electrical Eng. & Systems 2022-11-29 Royden Wagner , Karl Rohr

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

Image and Video Processing · Electrical Eng. & Systems 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard

Microfluidic devices offer numerous advantages in medical applications, including the capture of single cells in microwell-based platforms for genomic analysis. As the cost of sequencing decreases, the demand for high-throughput single-cell…

Computational Engineering, Finance, and Science · Computer Science 2024-09-13 Xueying Zhao , Yan Chen , Yuefu Jiang , Amie Radenbaugh , Jamie Moskwa , Devon Jensen

Cell detection in microscopy images is important to study how cells move and interact with their environment. Most recent deep learning-based methods for cell detection use convolutional neural networks (CNNs). However, inspired by the…

Image and Video Processing · Electrical Eng. & Systems 2022-06-15 Royden Wagner , Karl Rohr

The number of objects is considered an important factor in a variety of tasks in the agricultural domain. Automated counting can improve farmers decisions regarding yield estimation, stress detection, disease prevention, and more. In recent…

Computer Vision and Pattern Recognition · Computer Science 2023-05-10 Guy Farjon , Liu Huijun , Yael Edan

Accurate and efficient cell detection is crucial in many biomedical image analysis tasks. We evaluate the performance of several Deep Learning (DL) methods for cell detection in Papanicolaou-stained cytological Whole Slide Images (WSIs),…

Computer Vision and Pattern Recognition · Computer Science 2026-01-15 Marco Acerbis , Nataša Sladoje , Joakim Lindblad

Cell detection is an essential task in cell image analysis. Recent deep learning-based detection methods have achieved very promising results. In general, these methods require exhaustively annotating the cells in an entire image. If some…

Computer Vision and Pattern Recognition · Computer Science 2021-07-22 Kazuma Fujii , Daiki Suehiro , Kazuya Nishimura , Ryoma Bise

We present a scalable method for brain cell identification in multiview confocal light sheet microscopy images. Our algorithmic pipeline includes a hierarchical registration approach and a novel multiview version of semantic deconvolution…

Computer Vision and Pattern Recognition · Computer Science 2015-11-05 Marco Paciscopi , Ludovico Silvestri , Francesco Saverio Pavone , Paolo Frasconi

Counting plant organs such as heads or tassels from outdoor imagery is a popular benchmark computer vision task in plant phenotyping, which has been previously investigated in the literature using state-of-the-art supervised deep learning…

Computer Vision and Pattern Recognition · Computer Science 2020-07-21 Jordan Ubbens , Tewodros Ayalew , Steve Shirtliffe , Anique Josuttes , Curtis Pozniak , Ian Stavness

Fluorescence microscopy plays a vital role in understanding the subcellular structures of living cells. However, it requires considerable effort in sample preparation related to chemical fixation, staining, cost, and time. To reduce those…

Image and Video Processing · Electrical Eng. & Systems 2020-12-02 Thanh Nguyen , Vy Bui , Anh Thai , Van Lam , Christopher B. Raub , Lin-Ching Chang , George Nehmetallah

The use of fluorescent molecules to create long sequences of low-density, diffraction-limited images enables highly-precise molecule localization. However, this methodology requires lengthy imaging times, which limits the ability to view…

Image and Video Processing · Electrical Eng. & Systems 2024-03-26 Yair Ben Sahel , Yonina C. Eldar

The need to count and localize repeating objects in an image arises in different scenarios, such as biological microscopy studies, production lines inspection, and surveillance recordings analysis. The use of supervised Convoutional Neural…

Computer Vision and Pattern Recognition · Computer Science 2021-11-17 Inbar Huberman-Spiegelglas , Raanan Fattal

We present a new annotated microscopic cellular image dataset to improve the effectiveness of machine learning methods for cellular image analysis. Cell counting is an important step in cell analysis. Typically, domain experts manually…

Image and Video Processing · Electrical Eng. & Systems 2024-11-20 Abdurahman Ali Mohammed , Catherine Fonder , Donald S. Sakaguchi , Wallapak Tavanapong , Surya K. Mallapragada , Azeez Idris

Accurate cell detection and counting in the image-based ELISpot and FluoroSpot immunoassays is a challenging task. Recently proposed methodology matches human accuracy by leveraging knowledge of the underlying physical process of these…

Image and Video Processing · Electrical Eng. & Systems 2019-04-23 Pol del Aguila Pla , Vidit Saxena , Joakim Jaldén

Separating and labeling each instance of a nucleus (instance-aware segmentation) is the key challenge in segmenting single cell nuclei on fluorescence microscopy images. Deep Neural Networks can learn the implicit transformation of a…

Computer Vision and Pattern Recognition · Computer Science 2021-08-11 Florian Kromp , Lukas Fischer , Eva Bozsaky , Inge Ambros , Wolfgang Doerr , Sabine Taschner-Mandl , Peter Ambros , Allan Hanbury

Deep learning models have the capacity to fundamentally revolutionize medical imaging analysis, and they have particularly interesting applications in computer-aided diagnosis. We attempt to use deep learning neural networks to diagnose…

Machine Learning · Computer Science 2020-02-24 Rohit Jammula , Vishnu Rajan Tejus , Shreya Shankar