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We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM).…

Optics · Physics 2018-01-04 Seungwoo Shin , Doyeon Kim , Kyoohyun Kim , Yongkeun Park

In this paper, we review spatial light interference microscopy (SLIM), a common-path, phase-shifting interferometer, built onto a phase-contrast microscope, with white-light illumination. As one of the most sensitive quantitative phase…

Optics · Physics 2020-12-17 Xi Chen , Mikhail E. Kandel , Gabriel Popescu

In this paper, a method for increasing the temporal resolution of a temporal imaging system has been developed. Analogously to the conventional spatial imaging systems in which resolution limit is due to the finite aperture of the lens, in…

Optics · Physics 2020-04-22 Farshid Shateri , Mehdi Hosseinalizadeh , Zahra Kavehvash

Microscopy image enhancement plays a pivotal role in understanding the details of biological cells and materials at microscopic scales. In recent years, there has been a significant rise in the advancement of microscopy image enhancement,…

Image and Video Processing · Electrical Eng. & Systems 2025-09-29 Debasish Dutta , Neeharika Sonowal , Risheraj Barauh , Deepjyoti Chetia , Sanjib Kr Kalita

The use of the structural similarity index (SSIM) is widespread. For almost two decades, it has played a major role in image quality assessment in many different research disciplines. Clearly, its merits are indisputable in the research…

Image and Video Processing · Electrical Eng. & Systems 2020-07-01 Jim Nilsson , Tomas Akenine-Möller

This thesis centres on the development of multidimensional fluorescence imaging tools, with a particular emphasis on fluorescence lifetime imaging (FLIM) microscopy for application to biological research. The key aspects of this thesis are…

Instrumentation and Detectors · Physics 2017-08-14 Egidijus Auksorius

Super-Resolution Microscopy (SRM) is emerging as a powerful and innovative tool for imaging, characterizing, and understanding the structure of Extracellular Vesicles (EVs). By addressing the need for single-particle analysis with the high…

Microscopy is routinely used to image biological structures of interest. Due to imaging constraints, acquired images, also called as micrographs, are typically low-SNR and contain noise. Over the last few years, regression-based tasks like…

Image and Video Processing · Electrical Eng. & Systems 2024-10-16 Ashesh Ashesh , Joran Deschamps , Florian Jug

We present a study that evaluates the impact of regularization on the achieved resolution in restorations from a novel three-dimensional (3D) Structured Illumination Microscopy (3D-SIM) system with desirable tunability properties. This…

Image and Video Processing · Electrical Eng. & Systems 2024-03-22 Arash Atibi , Abdulaziz Alqahtani , Mohammed Younis , Chrysanthe Preza

Super-resolution microscopy overcomes the diffraction limit of conventional light microscopy in spatial resolution. By providing novel spatial or spatio-temporal information on biological processes at nanometer resolution with molecular…

Biological Physics · Physics 2021-11-10 Tianjie Yang , Yaoru Luo , Wei Ji , Ge Yang

Three-dimensional (3D) fluorescence imaging provides a vital approach for study of biological tissues with intricate structures, and optical sectioning structured illumination microscopy (OS-SIM) stands out for its high imaging speed, low…

We present a comprehensive guide to light-sheet microscopy (LSM) to assist scientists in navigating the practical implementation of this microscopy technique. Emphasizing the applicability of LSM to image both static microscale and…

This comprehensive review discusses the development of scanning electron microscopy and the application of this technology in different fields such as biology, nanobiotechnology and biomedical science. Besides being a tool for high…

Quantitative Methods · Quantitative Biology 2023-11-02 Aniruddha Acharya

A resolution-enhanced dark-field microscope by structured light illumination is proposed to improve resolution and contrast. A set of phase-shifted fringes are projected to the sample plane at large angle to capture modulated dark-field…

Image and Video Processing · Electrical Eng. & Systems 2022-02-14 Shaobai Li , Bofan Song , Rongguang Liang

Over the past decade, reflection matrix microscopy (RMM) and advanced image reconstruction algorithms have emerged to address the fundamental imaging depth limitations of optical microscopy in thick biological tissues and complex media. In…

Optics · Physics 2024-07-03 Sungsam Kang , Seokchan Yoon , Wonshik Choi

Fast and sensitive detector arrays enable image scanning microscopy (ISM), overcoming the trade-off between spatial resolution and signal-to-noise ratio (SNR) typical of confocal microscopy. However, current ISM approaches cannot provide…

Stacked intelligent metasurfaces (SIMs) have recently emerged as a key enabler for realizing electromagnetic wave-domain signal processing in next-generation wireless networks. However, practical SIM implementations often suffer from…

Signal Processing · Electrical Eng. & Systems 2026-03-10 Xing Jia , Hao Liu , Haoxian Niu , Jinbao Li , Xiangyu Ding , Lu Gan

It is now generally accepted that Euclidean-based metrics may not always adequately represent the subjective judgement of a human observer. As a result, many image processing methodologies have been recently extended to take advantage of…

Optimization and Control · Mathematics 2020-02-10 D. Otero , D. La Torre , O. Michailovich , E. R. Vrscay

In fluid flow imaging, intensity gradients are a good measure of spatial variations in scalar properties, which play an important role in controlling transport processes. However, current flow imaging techniques exhibit system-limited…

Optics · Physics 2025-10-22 Hy Cao , Abhishek Saha , Lisa V. Poulikakos

When capturing images in low-light conditions, the images often suffer from low visibility, which not only degrades the visual aesthetics of images, but also significantly degenerates the performance of many computer vision algorithms. In…

Computer Vision and Pattern Recognition · Computer Science 2020-12-17 Lijun Zhang , Xiao Liu , Erik Learned-Miller , Hui Guan