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Homeodomain containing proteins are a broad class of DNA binding proteins that are believed to primarily function as transcription factors. Electrostatics interactions have been demonstrated to be critical for the binding of the homeodomain…

Biomolecules · Quantitative Biology 2009-07-29 Christopher M. Frenz , Philippe P. Lefebvre

The ability to precisely control surface charge using charged polymers is fundamental to many nanotechnology applications, enabling the design and fabrication of materials with tailored properties and functionalities. Here, we study the…

Soft Condensed Matter · Physics 2025-12-09 Vijay Yadav , Prabhat Kumar Jaiswal , Rudolf Podgornik , Sunita Kumari

Hydrogen bond networks play vital roles in biological functions ranging from protein folding to enzyme catalysis. Here we combine electronic structure calculations and ab initio path integral molecular dynamics simulations, which…

Biological Physics · Physics 2017-08-17 Lu Wang , Stephen D. Fried , Thomas E. Markland

Multisite covalent modification of proteins is omnipresent in eukaryotic cells. A well-known example is the mitogen-activated protein kinase (MAPK) cascade, where in each layer of the cascade a protein is phosphorylated at two sites. It has…

Molecular Networks · Quantitative Biology 2015-05-13 Koichi Takahashi , Sorin Tanase-Nicola , Pieter Rein ten Wolde

Kinetic proofreading is an error correction mechanism present in the processes of the central dogma and beyond, and typically requires the free energy of nucleotide hydrolysis for its operation. Though the molecular players of many…

Molecular Networks · Quantitative Biology 2020-05-28 Vahe Galstyan , Rob Phillips

Allosteric transcription factors undergo binding events both at their inducer binding sites as well as at distinct DNA binding domains, and it is often difficult to disentangle the structural and functional consequences of these two classes…

Biomolecules · Quantitative Biology 2018-11-21 Tal Einav , Julia Duque , Rob Phillips

We study the behavior of negatively charged colloids with two positively charged polar caps close to a planar patterned surface. The competition between the different anisotropic components of the particle-particle interaction patterns is…

Soft Condensed Matter · Physics 2018-09-25 Emanuele Locatelli , Emanuela Bianchi

In the cell, proteins fold and perform complex functions through global structural rearrangements. Function requires a protein to be at the brink of stability to be susceptible to small environmental fluctuations, yet stable enough to…

Investigation of protein self-assembly processes is important for the understanding of the growth processes of functional proteins as well as disease-causing amyloids. Inside cells, intrinsic molecular fluctuations are so high that they…

Biological Physics · Physics 2021-02-03 John Bridstrup , John S Schreck , Jesse L Jorgenson , Jian-Min Yuan

Mutation is a critical mechanism by which evolution explores the functional landscape of proteins. Despite our ability to experimentally inflict mutations at will, it remains difficult to link sequence-level perturbations to systems-level…

Allostery is a fundamental mechanism of protein regulation and is commonly interpreted as modulating enzymatic activity or product abundance. Here we show that this view is incomplete. Using a stochastic model of allosteric regulation…

Molecular Networks · Quantitative Biology 2026-01-08 Pedro Pessoa , Steve Pressé , S. Banu Ozkan

The spatio-temporal organization of proteins and the associated morphological changes in membranes are of importance in cell signaling. Several mechanisms that promote the aggregation of proteins at low cell surface concentrations have been…

Biological Physics · Physics 2018-04-18 K. K. Sreeja , P. B. Sunil Kumar

Function of proteins or a network of interacting proteins often involves communication between residues that are well separated in sequence. The classic example is the participation of distant residues in allosteric regulation.…

Biomolecules · Quantitative Biology 2007-05-23 Ruxandra I. Dima , D. Thirumalai

In the presence of ATP, kinesin proceeds along the protofilament of microtubule by alternated binding of two motor domains on the tubulin binding sites. Since the processivity of kinesin is much higher than other motor proteins, it has been…

Biomolecules · Quantitative Biology 2009-11-13 Changbong Hyeon , Jose N. Onuchic

Allosterism traditionally refers to local changes in an extended object, for instance the binding of a ligand to a macromolecule, leading to a localized response at some other, possibly quite remote position. Here, we show that such…

Statistical Mechanics · Physics 2023-08-11 Christian Eidecker-Dunkel , Peter Reimann

G protein-coupled receptors (GPCRs) represent a diverse and vital family of membrane proteins that mediate intracellular signaling in response to extracellular stimuli, playing critical roles in physiology and disease. Traditionally…

Biological Physics · Physics 2025-06-04 Aakanksha J Shetty , Alexei Sirbu , Paolo Annibale

The functions of most genetic circuits require sufficient degrees of cooperativity in the circuit components. While mechanisms of cooperativity have been studied most extensively in the context of transcriptional initiation control,…

Molecular Networks · Quantitative Biology 2009-11-11 Nicolas E. Buchler , Ulrich Gerland , Terence Hwa

Correlated motions of proteins underpin many physiological mechanisms, such as substrate binding, signal transduction, enzymatic activity and allostery. These motions arise from low frequency collective movements of biomolecules and have…

Quantitative Methods · Quantitative Biology 2025-10-07 Ana Milinski , Annick Dejaegere , Roland Stote

We report on a theoretical study of point mutations effects on charge transfer properties in the DNA sequence of the tumor-suppressor p53 gene. On the basis of effective single-strand or double-strand tight-binding models which simulate…

Genomics · Quantitative Biology 2008-01-09 Chi-Tin Shih , Stephan Roche , Rudolf A. Römer

The Spatial Convolution Model (SCM) analyzes allostery based on the spatial evolution of the docking protein elastic media, whereby convolution of the media in response to wave propagation is solved as a function of Z fluctuations and…

Biological Physics · Physics 2021-03-18 Leroy K. Davis