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Digital neuron reconstruction from 3D microscopy images is an essential technique for investigating brain connectomics and neuron morphology. Existing reconstruction frameworks use convolution-based segmentation networks to partition the…

Computer Vision and Pattern Recognition · Computer Science 2022-10-19 Runkai Zhao , Heng Wang , Chaoyi Zhang , Weidong Cai

Convolutional neural networks (CNNs) are usually used as a backbone to design methods in biomedical image segmentation. However, the limitation of receptive field and large number of parameters limit the performance of these methods. In…

Image and Video Processing · Electrical Eng. & Systems 2022-09-27 Chong Wu , Zhenan Feng , Houwang Zhang , Hong Yan

Segmentation of white matter lesions and deep grey matter structures is an important task in the quantification of magnetic resonance imaging in multiple sclerosis. In this paper we explore segmentation solutions based on convolutional…

Motivation: 3D neuron segmentation is a key step for the neuron digital reconstruction, which is essential for exploring brain circuits and understanding brain functions. However, the fine line-shaped nerve fibers of neuron could spread in…

Image and Video Processing · Electrical Eng. & Systems 2021-11-09 Qiufu Li , Linlin Shen

This study explores the potential of graph neural networks (GNNs) to enhance semantic segmentation across diverse image modalities. We evaluate the effectiveness of a novel GNN-based U-Net architecture on three distinct datasets: PascalVOC,…

Computer Vision and Pattern Recognition · Computer Science 2025-01-22 Aryan Singh , Pepijn Van de Ven , Ciarán Eising , Patrick Denny

Neuron reconstruction, one of the fundamental tasks in neuroscience, rebuilds neuronal morphology from 3D light microscope imaging data. It plays a critical role in analyzing the structure-function relationship of neurons in the nervous…

Computer Vision and Pattern Recognition · Computer Science 2024-05-07 Yik San Cheng , Runkai Zhao , Heng Wang , Hanchuan Peng , Weidong Cai

Semantic image segmentation is one of the most important tasks in medical image analysis. Most state-of-the-art deep learning methods require a large number of accurately annotated examples for model training. However, accurate annotation…

Computer Vision and Pattern Recognition · Computer Science 2020-04-21 Ning Zhang , Susan Francis , Rayaz Malik , Xin Chen

Existing learning-based methods to automatically trace axons in 3D brain imagery often rely on manually annotated segmentation labels. Labeling is a labor-intensive process and is not scalable to whole-brain analysis, which is needed for…

Image and Video Processing · Electrical Eng. & Systems 2020-04-22 Tzofi Klinghoffer , Peter Morales , Young-Gyun Park , Nicholas Evans , Kwanghun Chung , Laura J. Brattain

Object segmentation and structure localization are important steps in automated image analysis pipelines for microscopy images. We present a convolution neural network (CNN) based deep learning architecture for segmentation of objects in…

Computer Vision and Pattern Recognition · Computer Science 2019-01-24 Shan E Ahmed Raza , Linda Cheung , Muhammad Shaban , Simon Graham , David Epstein , Stella Pelengaris , Michael Khan , Nasir M. Rajpoot

We consider the problem of finding an accurate representation of neuron shapes, extracting sub-cellular features, and classifying neurons based on neuron shapes. In neuroscience research, the skeleton representation is often used as a…

Computer Vision and Pattern Recognition · Computer Science 2022-12-15 Jiaxiang Jiang , Michael Goebel , Cezar Borba , William Smith , B. S. Manjunath

Background Analyzing images to accurately estimate the number of different cell types in the brain using automatic methods is a major objective in neuroscience. The automatic and selective detection and segmentation of neurons would be an…

Image and Video Processing · Electrical Eng. & Systems 2024-10-07 Antonio LaTorre , Lidia Alonso-Nanclares , José María Peña , Javier De Felipe

This work describes a novel methodology for automatic contour extraction from 2D images of 3D neurons (e.g. camera lucida images and other types of 2D microscopy). Most contour-based shape analysis methods can not be used to characterize…

Computer Vision and Pattern Recognition · Computer Science 2008-10-24 J. J. G. Leandro , R. M. Cesar , L. da F. Costa

Reconstructing neuron morphology from 3D light microscope imaging data is critical to aid neuroscientists in analyzing brain networks and neuroanatomy. With the boost from deep learning techniques, a variety of learning-based segmentation…

Image and Video Processing · Electrical Eng. & Systems 2025-02-18 Yik San Cheng , Runkai Zhao , Heng Wang , Hanchuan Peng , Yui Lo , Yuqian Chen , Lauren J. O'Donnell , Weidong Cai

Automatic 3D neuron reconstruction is critical for analysing the morphology and functionality of neurons in brain circuit activities. However, the performance of existing tracing algorithms is hinged by the low image quality. Recently, a…

Image and Video Processing · Electrical Eng. & Systems 2021-09-17 Heng Wang , Chaoyi Zhang , Jianhui Yu , Yang Song , Siqi Liu , Wojciech Chrzanowski , Weidong Cai

Reconstruction of neuroanatomy is a fundamental problem in neuroscience. Stochastic expression of colors in individual cells is a promising tool, although its use in the nervous system has been limited due to various sources of variability…

Neurons and Cognition · Quantitative Biology 2017-01-24 Uygar Sümbül , Douglas Roussien , Fei Chen , Nicholas Barry , Edward S. Boyden , Dawen Cai , John P. Cunningham , Liam Paninski

Automatic segmentation of fine-grained brain structures remains a challenging task. Current segmentation methods mainly utilize 2D and 3D deep neural networks. The 2D networks take image slices as input to produce coarse segmentation in…

Computer Vision and Pattern Recognition · Computer Science 2019-05-08 Yuemeng Li , Hangfan Liu , Hongming Li , Yong Fan

Cell nuclei segmentation is one of the most important tasks in the analysis of biomedical images. With ever-growing sizes and amounts of three-dimensional images to be processed, there is a need for better and faster segmentation methods.…

Computer Vision and Pattern Recognition · Computer Science 2017-02-20 Julian Arz , Peter Sanders , Johannes Stegmaier , Ralf Mikut

Semantic segmentation of neuronal structures in 3D high-resolution fluorescence microscopy imaging of the human brain cortex can take advantage of bidimensional CNNs, which yield good results in neuron localization but lead to inaccurate…

Image and Video Processing · Electrical Eng. & Systems 2020-09-03 Filippo Maria Castelli , Matteo Roffilli , Giacomo Mazzamuto , Irene Costantini , Ludovico Silvestri , Francesco Saverio Pavone

Due to imaging artifacts and low signal-to-noise ratio in ultrasound images, automatic bone surface segmentation networks often produce fragmented predictions that can hinder the success of ultrasound-guided computer-assisted surgical…

Image and Video Processing · Electrical Eng. & Systems 2022-06-20 Aimon Rahman , Wele Gedara Chaminda Bandara , Jeya Maria Jose Valanarasu , Ilker Hacihaliloglu , Vishal M Patel

Automatic segmentation of neuronal topology is critical for handling large scale neuroimaging data, as it can greatly accelerate neuron annotation and analysis. However, the intricate morphology of neuronal branches and the occlusions among…

Image and Video Processing · Electrical Eng. & Systems 2025-08-01 Huayu Fu , Jiamin Li , Haozhi Qu , Xiaolin Hu , Zengcai Guo
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