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Histochemical staining is essential for visualizing tissue architecture and cellular morphology but is destructive and limited by the availability of tissue for multiple stains. Virtual staining with label-free microscopy offers a…

This paper proposes a learning-based denoising method called FlashLight CNN (FLCNN) that implements a deep neural network for image denoising. The proposed approach is based on deep residual networks and inception networks and it is able to…

Image and Video Processing · Electrical Eng. & Systems 2020-07-06 Pham Huu Thanh Binh , Cristóvão Cruz , Karen Egiazarian

The prevalent convolutional neural network (CNN) based image denoising methods extract features of images to restore the clean ground truth, achieving high denoising accuracy. However, these methods may ignore the underlying distribution of…

Computer Vision and Pattern Recognition · Computer Science 2021-05-12 Yang Liu , Saeed Anwar , Zhenyue Qin , Pan Ji , Sabrina Caldwell , Tom Gedeon

Fluorescence microscopy allows for a detailed inspection of cells, cellular networks, and anatomical landmarks by staining with a variety of carefully-selected markers visualized as color channels. Quantitative characterization of…

Computer Vision and Pattern Recognition · Computer Science 2021-08-26 Alvaro Gomariz , Tiziano Portenier , Patrick M. Helbling , Stephan Isringhausen , Ute Suessbier , César Nombela-Arrieta , Orcun Goksel

Time-lapse fluorescent microscopy (TLFM) combined with predictive mathematical modelling is a powerful tool to study the inherently dynamic processes of life on the single-cell level. Such experiments are costly, complex and labour…

Computer Vision and Pattern Recognition · Computer Science 2021-09-27 Christoph Reich , Tim Prangemeier , Christian Wildner , Heinz Koeppl

Deep neural networks can generate images that are astonishingly realistic, so much so that it is often hard for humans to distinguish them from actual photos. These achievements have been largely made possible by Generative Adversarial…

Computer Vision and Pattern Recognition · Computer Science 2020-06-29 Joel Frank , Thorsten Eisenhofer , Lea Schönherr , Asja Fischer , Dorothea Kolossa , Thorsten Holz

Photoacoustic imaging has shown great potential for guiding minimally invasive procedures by accurate identification of critical tissue targets and invasive medical devices (such as metallic needles). The use of light emitting diodes (LEDs)…

Image and Video Processing · Electrical Eng. & Systems 2023-03-22 Mengjie Shi , Tianrui Zhao , Simeon J. West , Adrien E. Desjardins , Tom Vercauteren , Wenfeng Xia

The cost of some drugs and medical treatments has risen in recent years that many patients are having to go without. A classification project could make researchers more efficient. One of the more surprising reasons behind the cost is how…

Computer Vision and Pattern Recognition · Computer Science 2020-07-14 Manik Sharma , Ganapathy Krishnamurthi

Cell painting is a popular technique for creating human-interpretable, high-contrast images of cell morphology. There are two major issues with cell paint: (1) it is labor-intensive and (2) it requires chemical fixation, making the study of…

Computer Vision and Pattern Recognition · Computer Science 2025-12-16 Alexander Peysakhovich , William Berman , Joseph Rufo , Felix Wong , Maxwell Z. Wilson

Supervised deep learning algorithms have enabled significant performance gains in medical image classification tasks. But these methods rely on large labeled datasets that require resource-intensive expert annotation. Semi-supervised…

Microscopy images from different imaging conditions, organs, and tissues often have numerous cells with various shapes on a range of backgrounds. As a result, designing a deep learning model to count cells in a source domain becomes…

Computer Vision and Pattern Recognition · Computer Science 2023-03-21 Zuhui Wang

Cell shape analysis is important in biomedical research. Deep learning methods may perform to segment individual cells if they use sufficient training data that the boundary of each cell is annotated. However, it is very time-consuming for…

Image and Video Processing · Electrical Eng. & Systems 2020-02-26 Kazuya Nishimura , Dai Fei Elmer Ker , Ryoma Bise

Microtubule networks (MTs) are a component of a cell that may indicate the presence of various chemical compounds and can be used to recognize properties such as treatment resistance. Therefore, the classification of MT images is of great…

Image and Video Processing · Electrical Eng. & Systems 2020-12-23 Aleksei Shpilman , Dmitry Boikiy , Marina Polyakova , Daniel Kudenko , Anton Burakov , Elena Nadezhdina

Advanced image segmentation and processing tools present an opportunity to study cell processes and their dynamics. However, image analysis is often routine and time-consuming. Nowadays, alternative data-driven approaches using deep…

Volumetric cell segmentation in fluorescence microscopy images is important to study a wide variety of cellular processes. Applications range from the analysis of cancer cells to behavioral studies of cells in the embryonic stage. Like in…

Image and Video Processing · Electrical Eng. & Systems 2022-11-29 Royden Wagner , Karl Rohr

If light passes through the body tissues, focusing only on areas where treatment needs, such as tumors, will revolutionize many biomedical imaging and therapy technologies. So how to focus light through deep inhomogeneous tissues overcoming…

Image and Video Processing · Electrical Eng. & Systems 2020-08-18 Changchun Yang , Hengrong Lan , Fei Gao

Fluorescence labeling is the standard approach to reveal cellular structures and other subcellular constituents for microscopy images. However, this invasive procedure may perturb or even kill the cells and the procedure itself is highly…

Image and Video Processing · Electrical Eng. & Systems 2024-06-25 Han Liu , Hao Li , Jiacheng Wang , Yubo Fan , Zhoubing Xu , Ipek Oguz

The in vitro clonogenic assay is a technique to study the ability of a cell to form a colony in a culture dish. By optical imaging, dishes with stained colonies can be scanned and assessed digitally. Identification, segmentation and…

Computer Vision and Pattern Recognition · Computer Science 2022-02-15 Delmon Arous , Stefan Schrunner , Ingunn Hanson , Nina F. J. Edin , Eirik Malinen

Raman spectroscopy enables non-destructive, label-free molecular analysis with high specificity, making it a powerful tool for biomedical diagnostics. However, its application to biological tissues is challenged by inherently weak Raman…

Computer Vision and Pattern Recognition · Computer Science 2025-12-22 Mengkun Chen , Sanidhya D. Tripathi , James W. Tunnell

As AI-based medical devices are becoming more common in imaging fields like radiology and histology, interpretability of the underlying predictive models is crucial to expand their use in clinical practice. Existing heatmap-based…

Image and Video Processing · Electrical Eng. & Systems 2021-01-20 Kathryn Schutte , Olivier Moindrot , Paul Hérent , Jean-Baptiste Schiratti , Simon Jégou
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