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Staining is critical to cell imaging and medical diagnosis, which is expensive, time-consuming, labor-intensive, and causes irreversible changes to cell tissues. Recent advances in deep learning enabled digital staining via supervised model…

Image and Video Processing · Electrical Eng. & Systems 2023-03-06 Ziwang Xu , Lanqing Guo , Shuyan Zhang , Alex C. Kot , Bihan Wen

Fluorescence microscopy plays a vital role in understanding the subcellular structures of living cells. However, it requires considerable effort in sample preparation related to chemical fixation, staining, cost, and time. To reduce those…

Image and Video Processing · Electrical Eng. & Systems 2020-12-02 Thanh Nguyen , Vy Bui , Anh Thai , Van Lam , Christopher B. Raub , Lin-Ching Chang , George Nehmetallah

Using a deep neural network, we demonstrate a digital staining technique, which we term PhaseStain, to transform quantitative phase images (QPI) of labelfree tissue sections into images that are equivalent to brightfield microscopy images…

Image and Video Processing · Electrical Eng. & Systems 2019-02-08 Yair Rivenson , Tairan Liu , Zhensong Wei , Yibo Zhang , Aydogan Ozcan

Histological analysis of tissue samples is one of the most widely used methods for disease diagnosis. After taking a sample from a patient, it goes through a lengthy and laborious preparation, which stains the tissue to visualize different…

Computer Vision and Pattern Recognition · Computer Science 2019-03-05 Yair Rivenson , Hongda Wang , Zhensong Wei , Yibo Zhang , Harun Gunaydin , Aydogan Ozcan

Generally, microscopy image analysis in biology relies on the segmentation of individual nuclei, using a dedicated stained image, to identify individual cells. However stained nuclei have drawbacks like the need for sample preparation, and…

Image and Video Processing · Electrical Eng. & Systems 2022-11-08 Gendarme Mathieu , Lambert Annika M. , El Debs Bachir

Many medical and biological protocols for analyzing individual biological cells involve morphological evaluation based on cell staining, designed to enhance imaging contrast and enable clinicians and biologists to differentiate between…

Histological staining is the gold standard for tissue examination in clinical pathology and life-science research, which visualizes the tissue and cellular structures using chromatic dyes or fluorescence labels to aid the microscopic…

Medical Physics · Physics 2023-03-07 Bijie Bai , Xilin Yang , Yuzhu Li , Yijie Zhang , Nir Pillar , Aydogan Ozcan

Staining is essential in cell imaging and medical diagnostics but poses significant challenges, including high cost, time consumption, labor intensity, and irreversible tissue alterations. Recent advances in deep learning have enabled…

Computer Vision and Pattern Recognition · Computer Science 2025-04-15 Ziwang Xu , Lanqing Guo , Satoshi Tsutsui , Shuyan Zhang , Alex C. Kot , Bihan Wen

Deep learning-based virtual staining was developed to introduce image contrast to label-free tissue sections, digitally matching the histological staining, which is time-consuming, labor-intensive, and destructive to tissue. Standard…

Image and Video Processing · Electrical Eng. & Systems 2022-10-31 Yijie Zhang , Luzhe Huang , Tairan Liu , Keyi Cheng , Kevin de Haan , Yuzhu Li , Bijie Bai , Aydogan Ozcan

Virtual staining, or in-silico-labeling, has been proposed to computationally generate synthetic fluorescence images from label-free images by use of deep learning-based image-to-image translation networks. In most reported studies,…

Image and Video Processing · Electrical Eng. & Systems 2025-08-04 Sourya Sengupta , Jianquan Xu , Phuong Nguyen , Frank J. Brooks , Yang Liu , Mark A. Anastasio

Accurate and fast histological staining is crucial in histopathology, impacting diagnostic precision and reliability. Traditional staining methods are time-consuming and subjective, causing delays in diagnosis. Digital pathology plays a…

Image and Video Processing · Electrical Eng. & Systems 2023-07-06 Marian Boktor , James E. D. Tweel , Benjamin R. Ecclestone , Jennifer Ai Ye , Paul Fieguth , Parsin Haji Reza

Cell imaging assays utilizing fluorescence stains are essential for observing sub-cellular organelles and their responses to perturbations. Immunofluorescent staining process is routinely in labs, however the recent innovations in…

Visualizing the details of different cellular structures is of great importance to elucidate cellular functions. However, it is challenging to obtain high quality images of different structures directly due to complex cellular environments.…

Image and Video Processing · Electrical Eng. & Systems 2019-10-01 Yi Liu , Hao Yuan , Zhengyang Wang , Shuiwang Ji

Histological staining is a vital step used to diagnose various diseases and has been used for more than a century to provide contrast to tissue sections, rendering the tissue constituents visible for microscopic analysis by medical experts.…

Image and Video Processing · Electrical Eng. & Systems 2020-05-08 Yijie Zhang , Kevin de Haan , Yair Rivenson , Jingxi Li , Apostolos Delis , Aydogan Ozcan

Histopathological cancer diagnosis is based on visual examination of stained tissue slides. Hematoxylin and eosin (H\&E) is a standard stain routinely employed worldwide. It is easy to acquire and cost effective, but cells and tissue…

Computer Vision and Pattern Recognition · Computer Science 2022-07-12 Zhaoyang Xu , Xingru Huang , Carlos Fernández Moro , Béla Bozóky , Qianni Zhang

Automatic analysis of spatio-temporal microscopy images is inevitable for state-of-the-art research in the life sciences. Recent developments in deep learning provide powerful tools for automatic analyses of such image data, but heavily…

Image and Video Processing · Electrical Eng. & Systems 2021-01-28 Dennis Bähr , Dennis Eschweiler , Anuk Bhattacharyya , Daniel Moreno-Andrés , Wolfram Antonin , Johannes Stegmaier

Histopathological analysis is crucial in artery characterization for coronary artery disease (CAD). However, histology requires an invasive and time-consuming process. In this paper, we propose to generate virtual histology staining using…

Image and Video Processing · Electrical Eng. & Systems 2022-11-15 Xueshen Li , Hongshan Liu , Xiaoyu Song , Brigitta C. Brott , Silvio H. Litovsky , Yu Gan

Fourier ptychographic microscopy (FPM) is a computational approach geared towards creating high-resolution and large field-of-view images without mechanical scanning. To acquire color images of histology slides, it often requires sequential…

Image and Video Processing · Electrical Eng. & Systems 2020-10-28 Ruihai Wang , Pengming Song , Shaowei Jiang , Chenggang Yan , Jiakai Zhu , Chengfei Guo , Zichao Bian , Tianbo Wang , Guoan Zheng

Accurate single cell detection in brightfield microscopy is crucial for biological research, yet data scarcity and annotation bottlenecks limit the progress of deep learning methods. We investigate the use of unconditional models to…

Computer Vision and Pattern Recognition · Computer Science 2025-12-02 Mario de Jesus da Graca , Jörg Dahlkemper , Peer Stelldinger

The emergence of virtual staining technology provides a rapid and efficient alternative for researchers in tissue pathology. It enables the utilization of unlabeled microscopic samples to generate virtual replicas of chemically stained…

Image and Video Processing · Electrical Eng. & Systems 2025-01-08 Junjia Wang , Bo Xiong , You Zhou , Xun Cao , Zhan Ma
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