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Functional proteins must fold with some minimal stability to a structure that can perform a biochemical task. Here we use a simple model to investigate the relationship between the stability requirement and the capacity of a protein to…

Biomolecules · Quantitative Biology 2009-11-10 Jesse D Bloom , Claus O Wilke , Frances H Arnold , Christoph Adami

We investigate the sequence-dependent properties of proteins that determine the dual requirements of stability of the native state and its kinetic accessibility using simple cubic lattice models. Three interaction schemes are used to…

Soft Condensed Matter · Physics 2009-10-31 D. K. Klimov , D. Thirumalai

The rates of protein folding with photon absorption or emission and the cross section of photon -protein inelastic scattering are calculated from the quantum folding theory by use of standard field-theoretical method. All these protein…

Biomolecules · Quantitative Biology 2011-05-13 Liaofu Luo

We consider the statistical mechanics of a full set of two-dimensional protein-like heteropolymers, whose thermodynamics is characterized by the coil-to-globular ($T_\theta$) and the folding ($T_f$) transition temperatures. For our model,…

chem-ph · Physics 2009-10-28 Carlos J. Camacho , D. Thirumalai

Mechanical unfolding trajectories, generated by applying constant force in optical tweezer experiments, show that RNA hairpins and the P5abc subdomain of the group I intron unfold reversibly. We use coarse-grained Go-like models for RNA…

Biomolecules · Quantitative Biology 2009-11-11 Changbong Hyeon , D. Thirumalai

A coarse-grained variational model is used to investigate the polymer dynamics of barrier crossing for a diverse set of two-state folding proteins. The model gives reliable folding rate predictions provided excluded volume terms that induce…

Biomolecules · Quantitative Biology 2009-11-13 Xianghong Qi , John J. Portman

We study the thermodynamic and kinetic consequences of the competition between single-protein folding and protein-protein aggregation using a phenomenological model, in which the proteins can be in the unfolded (U), misfolded (M) or folded…

Soft Condensed Matter · Physics 2009-10-29 Moumita Maiti , Madan Rao , Srikanth Sastry

We propose a general theory to describe the distribution of protein-folding transition paths. We show that transition paths follow a predictable sequence of high-free-energy transient states that are separated by free-energy barriers. Each…

Biomolecules · Quantitative Biology 2016-09-21 William M. Jacobs , Eugene I. Shakhnovich

Force-clamp spectroscopy reveals the unfolding and disulfide bond rupture times of single protein molecules as a function of the stretching force, point mutations and solvent conditions. The statistics of these times reveal whether the…

Biological Physics · Physics 2012-12-07 Herbert Lannon , Eric Vanden-Eijnden , Jasna Brujic

Two proteins, one belonging to the mainly alpha class and the other belonging to the alpha/beta class, are selected to test a kinetic mechanism for protein folding. Targeted molecular dynamics is applied to generate folding pathways for…

Biological Physics · Physics 2011-01-04 Leonor Cruzeiro

Mechanical stretching of six proteins is studied through molecular dynamics simulations. The model is Go-like, with Lennard-Jones interactions at native contacts. Low temperature unfolding scenarios are remarkably complex and sensitive to…

Biomolecules · Quantitative Biology 2009-11-10 Marek Cieplak , Trinh Xuan Hoang , Mark O. Robbins

Protein folding is a phenomenon that has been studied for about 50 years and still remains as an unsolved problem. The main feature of this process is that it occurs as an all or none process, so a protein, can jump directly between folded…

Biomolecules · Quantitative Biology 2020-09-07 German Mino-Galaz

A theoretical framework is developed to study the dynamics of protein folding. The key insight is that the search for the native protein conformation is influenced by the rate r at which external parameters, such as temperature, chemical…

Biomolecules · Quantitative Biology 2009-11-13 Gregg Lois , Jerzy Blawzdziewicz , Corey S. O'Hern

The thermodynamics of proteins indicate that folding/unfolding takes place either through stable intermediates or through a two-state process without intermediates. The rather short folding times of the two-state process indicate that…

Condensed Matter · Physics 2016-08-31 Audun Bakk , Johan S. Hoye , Alex Hansen , Kim Sneppen , Mogens Hogh Jensen

Using three-dimensional Go lattice models with side chains for proteins, we investigate the dependence of folding times on protein length. In agreement with previous theoretical predictions, we find that the folding time grows as a power…

Soft Condensed Matter · Physics 2007-05-23 Mai Suan Li , D. K. Klimov , D. Thirumalai

Thermal unfolding of proteins is compared to folding and mechanical stretching in a simple topology-based dynamical model. We define the unfolding time and demonstrate its low-temperature divergence. Below a characteristic temperature,…

Biomolecules · Quantitative Biology 2009-11-13 Marek Cieplak , Joanna I. Sulkowska

Topological phase transitions track changes in topological properties of a system and occur in real materials as well as quantum engineered systems, all of which differ greatly in terms of dimensionality, symmetries, interactions, and…

Statistical Mechanics · Physics 2020-04-02 Paolo Molignini , R. Chitra , Wei Chen

The simulation of a protein's folding process is often done via stochastic local search, which requires a procedure to apply structural changes onto a given conformation. Here, we introduce a constraint-based approach to enumerate lattice…

Computational Engineering, Finance, and Science · Computer Science 2009-10-21 Martin Mann , Mohamed Abou Hamra , Kathleen Steinhöfel , Rolf Backofen

The understanding, and even the description of protein folding is impeded by the complexity of the process. Much of this complexity can be described and understood by taking a statistical approach to the energetics of protein conformation,…

chem-ph · Physics 2008-02-03 J. D. Bryngelson , J. N. Onuchic , N. D. Socci , P. G. Wolynes

Single-molecule pulling experiments on unstructured proteins linked to neurodegenerative diseases have measured rupture forces comparable to those for stable folded proteins. To investigate the structural mechanisms of this unexpected force…

Biomolecules · Quantitative Biology 2013-06-19 S. Æ. Jónsson , S. Mitternacht , A. Irbäck
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