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We demonstrate that a deep neural network can significantly improve optical microscopy, enhancing its spatial resolution over a large field-of-view and depth-of-field. After its training, the only input to this network is an image acquired…

Machine Learning · Computer Science 2017-11-21 Yair Rivenson , Zoltan Gorocs , Harun Gunaydin , Yibo Zhang , Hongda Wang , Aydogan Ozcan

Cell segmentation in single-shot quantitative phase microscopy (ssQPM) faces challenges from traditional thresholding methods that are sensitive to noise and cell density, while deep learning approaches using simple channel concatenation…

Computer Vision and Pattern Recognition · Computer Science 2025-11-04 Rajatsubhra Chakraborty , Ana Espinosa-Momox , Riley Haskin , Depeng Xu , Rosario Porras-Aguilar

Oversmoothing remains a persistent problem when applying deep learning to off-axis quantitative phase imaging (QPI). End-to-end U-Nets favour low-frequency content and under-represent fine, diagnostic detail. We trace this issue to spectral…

Image and Video Processing · Electrical Eng. & Systems 2025-06-16 Yi Zhang

Unsupervised depth estimation from a single image is a very attractive technique with several implications in robotic, autonomous navigation, augmented reality and so on. This topic represents a very challenging task and the advent of deep…

Computer Vision and Pattern Recognition · Computer Science 2018-08-01 Matteo Poggi , Filippo Aleotti , Fabio Tosi , Stefano Mattoccia

High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…

Image and Video Processing · Electrical Eng. & Systems 2019-03-28 Li-Hao Yeh , Shwetadwip Chowdhury , Laura Waller

Photoacoustic microscopy (PAM) is an emerging imaging method combining light and sound. However, limited by the laser's repetition rate, state-of-the-art high-speed PAM technology often sacrifices spatial sampling density (i.e.,…

Image and Video Processing · Electrical Eng. & Systems 2021-04-09 Tri Vu , Anthony DiSpirito , Daiwei Li , Zixuan Zhang , Xiaoyi Zhu , Maomao Chen , Laiming Jiang , Dong Zhang , Jianwen Luo , Yu Shrike Zhang , Qifa Zhou , Roarke Horstmeyer , Junjie Yao

Quantitative phase microscopy (QPM) enables label-free measurement of local optical path length variations, providing critical insight into the structure and dynamics of transparent biological specimens. Here, a highly sensitive lateral…

We present a comprehensive overview of the Deep Image Prior (DIP) framework and its applications to image reconstruction in computed tomography. Unlike conventional deep learning methods that rely on large, supervised datasets, the DIP…

Image and Video Processing · Electrical Eng. & Systems 2026-02-24 Simon Arridge , Riccardo Barbano , Alexander Denker , Zeljko Kereta

Statistical shape modeling is an important tool to characterize variation in anatomical morphology. Typical shapes of interest are measured using 3D imaging and a subsequent pipeline of registration, segmentation, and some extraction of…

Computer Vision and Pattern Recognition · Computer Science 2018-10-02 Riddhish Bhalodia , Shireen Y. Elhabian , Ladislav Kavan , Ross T. Whitaker

Sample-induced aberrations and optical imperfections limit the resolution of fluorescence microscopy. Phase diversity is a powerful technique that leverages complementary phase information in sequentially acquired images with deliberately…

Two-dimensional (2D) materials have attracted extensive attention due to their unique characteristics and application potentials. Raman spectroscopy, as a rapid and non-destructive probe, exhibits distinct features and holds notable…

Applied Physics · Physics 2023-12-05 Yaping Qi , Dan Hu , Zhenping Wu , Ming Zheng , Guanghui Cheng , Yucheng Jiang , Yong P. Chen

We present an ultra-fast, precise, parameter-free method, which we term Deep-STORM, for obtaining super-resolution images from stochastically-blinking emitters, such as fluorescent molecules used for localization microscopy. Deep-STORM uses…

Optics · Physics 2018-05-03 Elias Nehme , Lucien E. Weiss , Tomer Michaeli , Yoav Shechtman

Medical imaging plays a vital role in modern diagnostics; however, interpreting high-resolution radiological data remains time-consuming and susceptible to variability among clinicians. Traditional image processing techniques often lack the…

Computer Vision and Pattern Recognition · Computer Science 2025-10-21 Melika Filvantorkaman , Maral Filvan Torkaman

In this work, we focus on the inverse medium scattering problem (IMSP), which aims to recover unknown scatterers based on measured scattered data. Motivated by the efficient direct sampling method (DSM) introduced in [23], we propose a…

Signal Processing · Electrical Eng. & Systems 2023-05-02 Jianfeng Ning , Fuqun Han , Jun Zou

Quantitative susceptibility mapping (QSM) is a valuable magnetic resonance imaging (MRI) contrast mechanism that has demonstrated broad clinical applications. However, the image reconstruction of QSM is challenging due to its ill-posed…

Image and Video Processing · Electrical Eng. & Systems 2021-01-29 Yang Gao , Xuanyu Zhu , Bradford A. Moffat , Rebecca Glarin , Alan H. Wilman , G. Bruce Pike , Stuart Crozier , Feng Liu , Hongfu Sun

Diffusion MRI (dMRI) is a valuable tool to map brain microstructure and connectivity by analyzing water molecule diffusion in tissue. However, acquiring dMRI data requires to capture multiple 3D brain volumes in a short time, often leading…

Image and Video Processing · Electrical Eng. & Systems 2025-08-29 Sedigheh Dargahi , Sylvain Bouix , Christian Desrosiers

Tracking single molecules is instrumental for quantifying the transport of molecules and nanoparticles in biological samples, e.g., in brain drug delivery studies. Existing intensity-based localisation methods are not developed for imaging…

Compressed Sensing MRI reconstructs images of the body's internal anatomy from undersampled measurements, thereby reducing scan time. Recently, deep learning has shown great potential for reconstructing high-fidelity images from highly…

Image and Video Processing · Electrical Eng. & Systems 2025-04-07 Armeet Singh Jatyani , Jiayun Wang , Aditi Chandrashekar , Zihui Wu , Miguel Liu-Schiaffini , Bahareh Tolooshams , Anima Anandkumar

We demonstrate that in situ coherent diffractive imaging (CDI), which harnesses the coherent interference between a strong and a weak beam illuminating a static and dynamic structure, can be a very dose-efficient imaging method. At low…

This paper proposes the design of decision models based on Computational Intelligence techniques applied to image sequences of dynamic laser speckle. These models aim to identify image regions of biological specimens illuminated by a…