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Modern imaging techniques at the molecular scale rely on utilizing novel coherent light sources like X-ray free electron lasers for the ultimate goal of visualizing such objects as individual biomolecules rather than crystals. Here, unlike…

Three-dimensional molecular imaging of living cells is essential for unraveling cellular metabolism and response to therapies. However, existing volumetric methods, including fluorescence microscopy and quantitative phase imaging, either…

Three-dimensional elements, with refractive index distribution structured at sub-wavelength scale, provide an expansive optical design space that can be harnessed for demonstrating multi-functional free-space optical devices. Here we…

Current methods for extracting intrinsic image components, such as reflectance and shading, primarily rely on statistical priors. These methods focus mainly on simple synthetic scenes and isolated objects and struggle to perform well on…

Computer Vision and Pattern Recognition · Computer Science 2024-11-27 Yixiong Yang , Shilin Hu , Haoyu Wu , Ramon Baldrich , Dimitris Samaras , Maria Vanrell

X-ray imaging is a fundamental tool of routine clinical diagnosis. Fluoroscopic imaging can further acquire X-ray images at video frame rates, thus enabling non-invasive in-vivo motion studies of joints, gastrointestinal tract, etc. For…

Image and Video Processing · Electrical Eng. & Systems 2018-10-22 Jacky C. K. Chow , Derek Lichti , Kathleen Ang , Gregor Kuntze , Gulshan Sharma , Janet Ronsky

Optical imaging below the limit of light diffraction offers an unprecedented opportunity to study outlook, organization, interactions or in-situ functioning of sub-micrometer, highly transparent objects such as subcellular structures in…

Label-free imaging approaches seek to simplify and augment histopathologic assessment by replacing the current practice of staining by dyes to visualize tissue morphology with quantitative optical measurements. Quantitative phase imaging…

Pixel size in cameras and other refractive imaging devices is typically limited by the free-space diffraction. However, a vast majority of semiconductor-based detectors are based on materials with substantially high refractive index. We…

Optics · Physics 2018-03-28 Bo Fan , Sandeep Inampudi , Viktor A. Podolskiy

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

Optics · Physics 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

The standard approach to the analysis of functional magnetic resonance imaging (FMRI) data applies various preprocessing steps to the original FMRI. These preprocessings lead to a general underestimation of residual variance in the…

Applications · Statistics 2018-09-20 Thomas Wilhelm Dieter Möbius

Microstructure characterisation has been greatly enhanced through the use of electron backscatter diffraction (EBSD), where rich maps are generated through analysis of the crystal phase and orientation in the scanning electron microscope…

Materials Science · Physics 2018-11-15 Vivian S Tong , Alexander J Knowles , David Dye , T Ben Britton

Confocal Raman microscopy, a highly specific and label-free technique for the microscale study of thick samples, often presents difficulties due to weak Raman signals. Inhomogeneous samples introduce wavefront aberrations that further…

Interaction-free measurement (IFM) has been proposed as a means of high-resolution, low-damage imaging of radiation-sensitive samples, such as biomolecules and proteins. The basic setup for IFM is a Mach-Zehnder interferometer, and recent…

Instrumentation and Detectors · Physics 2019-06-12 Akshay Agarwal , Karl K. Berggren , Vivek Goyal

Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds…

Whole slide imaging (WSI) has moved digital pathology closer to diagnostic practice in recent years. Due to the inherent tissue topography variability, accurate autofocusing remains a critical challenge for WSI and automated microscopy…

We introduce the Divergence Phase Index (DPI), a novel framework for quantifying phase differences in one and multidimensional signals, grounded in harmonic analysis via the Riesz transform. Based on classical Hilbert Transform phase…

Machine Learning · Statistics 2025-10-07 Magaly Catanzariti , Hugo Aimar , Diego M. Mateos

Microscopy enables direct observation of cellular morphology in 3D, with transmitted-light methods offering low-cost, minimally invasive imaging and fluorescence microscopy providing specificity and contrast. Virtual staining combines these…

Magnetic resonance imaging (MRI), especially functional MRI (fMRI) and diffusion MRI (dMRI), is essential for studying neurodegenerative diseases. However, missing modalities pose a major barrier to their clinical use. Although GAN- and…

Computer Vision and Pattern Recognition · Computer Science 2025-11-10 Xiongri Shen , Jiaqi Wang , Yi Zhong , Zhenxi Song , Leilei Zhao , Yichen Wei , Lingyan Liang , Shuqiang Wang , Baiying Lei , Demao Deng , Zhiguo Zhang

Fluorescence microscopy has been a significant tool to observe long-term imaging of embryos (in vivo) growth over time. However, cumulative exposure is phototoxic to such sensitive live samples. While techniques like light-sheet…

Image and Video Processing · Electrical Eng. & Systems 2022-01-05 Varun Mannam , Jacob Brandt , Cody J. Smith , Scott Howard