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We consider the problem of accurately identifying cell boundaries and labeling individual cells in confocal microscopy images, specifically, 3D image stacks of cells with tagged cell membranes. Precise identification of cell boundaries,…

Computer Vision and Pattern Recognition · Computer Science 2019-09-06 Jiaxiang Jiang , Po-Yu Kao , Samuel A. Belteton , Daniel B. Szymanski , B. S. Manjunath

The automated analysis of microscopy images is a challenge in the context of single-cell tracking and quantification. This work has as goals the study of the performance of deep learning for segmenting microscopy images and the improvement…

Quantitative Methods · Quantitative Biology 2022-10-05 André O. Françani

Counting immunopositive cells on biological tissues generally requires either manual annotation or (when available) automatic rough systems, for scanning signal surface and intensity in whole slide imaging. In this work, we tackle the…

Computational Engineering, Finance, and Science · Computer Science 2026-02-27 L. Martino , M. M. Garcia , P. S. Paradas , E. Curbelo

Object segmentation and structure localization are important steps in automated image analysis pipelines for microscopy images. We present a convolution neural network (CNN) based deep learning architecture for segmentation of objects in…

Computer Vision and Pattern Recognition · Computer Science 2019-01-24 Shan E Ahmed Raza , Linda Cheung , Muhammad Shaban , Simon Graham , David Epstein , Stella Pelengaris , Michael Khan , Nasir M. Rajpoot

Automated cell detection and localization from microscopy images are significant tasks in biomedical research and clinical practice. In this paper, we design a new cell detection and localization algorithm that combines deep convolutional…

Computer Vision and Pattern Recognition · Computer Science 2018-10-09 Yao Xue , Gilbert Bigras , Judith Hugh , Nilanjan Ray

Sickle cell anemia, which is characterized by abnormal erythrocyte morphology, can be detected using microscopic images. Computational techniques in medicine enhance the diagnosis and treatment efficiency. However, many computational…

Computer Vision and Pattern Recognition · Computer Science 2024-12-25 Victor Júnio Alcântara Cardoso , Rodrigo Moreira , João Fernando Mari , Larissa Ferreira Rodrigues Moreira

Combining experiments with artificial intelligence algorithms, we propose a new machine learning based approach to extract the cellular force distributions from the microscope images. The full process can be divided into three steps. First,…

Purpose: The aim of this work is to demonstrate that convolutional neural networks (CNN) can be applied to extremely sparse image libraries by subdivision of the original image datasets. Methods: Image datasets from a conventional digital…

Computer Vision and Pattern Recognition · Computer Science 2020-10-27 Johan P. Boetker

Cell segmentation and tracking in microscopy images are of great significance to new discoveries in biology and medicine. In this study, we propose a novel approach to combine cell segmentation and cell tracking into a unified end-to-end…

Computer Vision and Pattern Recognition · Computer Science 2021-02-23 Yuqian Chen , Yang Song , Chaoyi Zhang , Fan Zhang , Lauren O'Donnell , Wojciech Chrzanowski , Weidong Cai

High-resolution (HR) magnetic resonance images (MRI) provide detailed anatomical information important for clinical application and quantitative image analysis. However, HR MRI conventionally comes at the cost of longer scan time, smaller…

Computer Vision and Pattern Recognition · Computer Science 2018-06-12 Yuhua Chen , Feng Shi , Anthony G. Christodoulou , Zhengwei Zhou , Yibin Xie , Debiao Li

In recent years, Deep Learning (DL) has shown promising results in conducting AI tasks such as computer vision and image segmentation. Specifically, Convolutional Neural Network (CNN) models in DL have been applied to prevention,detection,…

Image and Video Processing · Electrical Eng. & Systems 2019-11-19 Ahmed Awad Albishri , Syed Jawad Hussain Shah , Anthony Schmiedler , Seung Suk Kang , Yugyung Lee

Crowd counting on static images is a challenging problem due to scale variations. Recently deep neural networks have been shown to be effective in this task. However, existing neural-networks-based methods often use the multi-column or…

Computer Vision and Pattern Recognition · Computer Science 2017-02-09 Lingke Zeng , Xiangmin Xu , Bolun Cai , Suo Qiu , Tong Zhang

Image segmentation is the foundation of several computer vision tasks, where pixel-wise knowledge is a prerequisite for achieving the desired target. Deep learning has shown promising performance in supervised image segmentation. However,…

Image and Video Processing · Electrical Eng. & Systems 2024-03-19 Boujemaa Guermazi , Riadh Ksantini , Naimul Khan

Deep neural networks have exhibited remarkable performance in image super-resolution (SR) tasks by learning a mapping from low-resolution (LR) images to high-resolution (HR) images. However, the SR problem is typically an ill-posed problem…

Computer Vision and Pattern Recognition · Computer Science 2024-05-29 Yong Guo , Mingkui Tan , Zeshuai Deng , Jingdong Wang , Qi Chen , Jiezhang Cao , Yanwu Xu , Jian Chen

Cell imaging and analysis are fundamental to biomedical research because cells are the basic functional units of life. Among different cell-related analysis, cell counting and detection are widely used. In this paper, we focus on one common…

Computer Vision and Pattern Recognition · Computer Science 2019-04-19 Haoyi Liang , Aijaz Naik , Cedric L. Williams , Jaideep Kapur , Daniel S. Weller

Artificial intelligence is nowadays used for cell detection and classification in optical microscopy, during post-acquisition analysis. The microscopes are now fully automated and next expected to be smart, to make acquisition decisions…

Cell counting remains a fundamental yet challenging task in medical and biological research due to the diverse morphology of cells, their dense distribution, and variations in image quality. We present DLA-Count, a breakthrough approach to…

Computer Vision and Pattern Recognition · Computer Science 2025-03-18 Yuqing Yan , Yirui Wu

Single cell segmentation is critical and challenging in live cell imaging data analysis. Traditional image processing methods and tools require time-consuming and labor-intensive efforts of manually fine-tuning parameters. Slight variations…

Quantitative Methods · Quantitative Biology 2019-04-24 Weikang Wang , David A. Taft , Yi-Jiun Chen , Jingyu Zhang , Callen T. Wallace , Min Xu , Simon C. Watkins , Jianhua Xing

Microscopy images are powerful tools and widely used in the majority of research areas, such as biology, chemistry, physics and materials fields by various microscopies (scanning electron microscope (SEM), atomic force microscope (AFM) and…

Computer Vision and Pattern Recognition · Computer Science 2020-02-25 Israel Goytom , Qin Wang , Tianxiang Yu , Kunjie Dai , Kris Sankaran , Xinfei Zhou , Dongdong Lin

We present a new annotated microscopic cellular image dataset to improve the effectiveness of machine learning methods for cellular image analysis. Cell counting is an important step in cell analysis. Typically, domain experts manually…

Image and Video Processing · Electrical Eng. & Systems 2024-11-20 Abdurahman Ali Mohammed , Catherine Fonder , Donald S. Sakaguchi , Wallapak Tavanapong , Surya K. Mallapragada , Azeez Idris