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We report a new coherent imaging technique, termed ptychographic structured modulation (PSM), for quantitative super-resolution microscopy. In this technique, we place a thin diffuser (i.e., a scattering lens) in between the sample and the…

Image and Video Processing · Electrical Eng. & Systems 2019-07-26 Pengming Song , Shaowei Jiang , He Zhang , Zichao Bian , Chengfei Guo , Kazunori Hoshino , Guoan Zheng

In this article, we report an imaging method, termed Fourier ptychographic microscopy (FPM), which iteratively stitches together a number of variably illuminated, low-resolution intensity images in Fourier space to produce a wide-field,…

Optics · Physics 2014-05-02 Guoan Zheng , Roarke Horstmeyer , Changhuei Yang

The recent introduction of portable, low-field MRI (LF-MRI) into the clinical setting has the potential to transform neuroimaging. However, LF-MRI is limited by lower resolution and signal-to-noise ratio, leading to incomplete…

Functional ultrasound imaging (fUSI) is a cutting-edge technology that measures changes in cerebral blood volume (CBV) by detecting backscattered echoes from red blood cells moving within its field of view (FOV). It offers high…

Neurons and Cognition · Quantitative Biology 2025-08-20 Jared Deighton , Shan Zhong , Kofi Agyeman , Wooseong Choi , Charles Liu , Darrin Lee , Vasileios Maroulas , Vasileios Christopoulos

Ultra High Frequency Ultrasound (UHFUS) enables the visualization of highly deformable small and medium vessels in the hand. Intricate vessel-based measurements, such as intimal wall thickness and vessel wall compliance, require…

Computer Vision and Pattern Recognition · Computer Science 2018-07-25 Tejas Sudharshan Mathai , Lingbo Jin , Vijay Gorantla , John Galeotti

A major open challenge in neuroscience is the ability to measure and perturb neural activity in vivo from well-defined neural sub-populations at cellular resolution anywhere in the brain. However, limitations posed by scattering and…

Biological Physics · Physics 2017-11-10 Shay Ohayon , Antonio Miguel Caravaca-Aguirre , Rafael Piestun , James J. DiCarlo

Microscopy imaging is vital in biology research and diagnosis. When imaging at the scale of cell or molecule level, mechanical drift on the axial axis can be difficult to correct. Although multi-scale networks have been developed for…

Image and Video Processing · Electrical Eng. & Systems 2023-05-31 Jiahe Wang , Boran Han

Ultrasound Localization Microscopy (ULM) has recently enabled the mapping of the cerebral vasculature in vivo with a resolution ten times smaller than the wavelength used, down to ten microns. However, with frame rates up to 20.000 frames…

Medical Physics · Physics 2023-10-23 Erwan Hardy , Jonathan Poree , Hatim Belgharbi , Chloe Bourquin , Frederic Lesage , Jean Provost

Fluorescence microscopy is widely used for the study of biological specimens. Deconvolution can significantly improve the resolution and contrast of images produced using fluorescence microscopy; in particular, Bayesian-based methods have…

Methodology · Statistics 2015-02-04 Alexander Wong , Xiao Yu Wang , Maud Gorbet

Light sheet fluorescence microscopy is able to image large specimen with high resolution by imaging the sam- ples from multiple angles. Multi-view deconvolution can significantly improve the resolution and contrast of the images, but its…

Quantitative Methods · Quantitative Biology 2014-12-03 Stephan Preibisch , Fernando Amat , Evangelia Stamataki , Mihail Sarov , Robert H. Singer , Eugene Myers , Pavel Tomancak

We present a photoacoustic computed tomography investigation on a healthy human finger, to image blood vessels with a focus on vascularity across the interphalangeal joints. The cross-sectional images were acquired using an imager…

Ultrasound vascular imaging is limited by acoustic diffraction, restricting visualization of microvessels essential for understanding organ function and disease. Label-free super-resolution methods exploiting endogenous red blood cells have…

Multifocus microscopy enables recording of entire volumes in a single camera exposure. In dense samples, multifocus microscopy is severely hampered by background haze. Here, we introduce a scalable multifocus method that incorporates…

The first choice in diagnostic imaging for patients suffering from peripheral arterial disease is 2D ultrasound (US). However, for a proper imaging process, a skilled and experienced sonographer is required. Additionally, it is a highly…

Photoacoustic tomography is a hybrid biomedical technology, which combines the advantages of acoustic and optical imaging. However, for the conventional image reconstruction method, the image quality is affected obviously by artifacts under…

Image and Video Processing · Electrical Eng. & Systems 2024-06-26 Bowei Yao , Yi Zeng , Haizhao Dai , Qing Wu , Youshen Xiao , Fei Gao , Yuyao Zhang , Jingyi Yu , Xiran Cai

Fourier ptychographic microscopy (FPM) is a novel computational coherent imaging technique for high space-bandwidth product imaging. Mathematically, Fourier ptychographic (FP) reconstruction can be implemented as a phase retrieval…

Computer Vision and Pattern Recognition · Computer Science 2016-07-19 Liheng Bian , Jinli Suo , Jaebum Chung , Xiaoze Ou , Changhuei Yang , Feng Chen , Qionghai Dai

As a general rule of thumb the resolution of a light microscope (i.e. the ability to discern objects) is predominantly described by the full width at half maximum (FWHM) of its point spread function (psf)---the diameter of the blurring…

Statistics Theory · Mathematics 2020-10-23 Gytis Kulaitis , Axel Munk , Frank Werner

Poor resolution of ultrasound images due to convolution of the tissue reflectivity function (TRF) with the system point spread function (PSF) is a major issue in medical ultrasound imaging. In this paper, we propose a correlation…

Signal Processing · Electrical Eng. & Systems 2018-06-08 Jayanta Dey , Md. Kamrul Hasan

We present STARRED, a Point Spread Function (PSF) reconstruction, two-channel deconvolution, and light curve extraction method designed for high-precision photometric measurements in imaging time series. An improved resolution of the data…

Instrumentation and Methods for Astrophysics · Physics 2024-07-25 Martin Millon , Kevin Michalewicz , Frédéric Dux , Frédéric Courbin , Philip J. Marshall

Acoustic holographic lenses, also known as acoustic holograms, can change the phase of a transmitted wavefront in order to shape and construct complex ultrasound pressure fields, often for focusing the acoustic energy on a target region.…