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Multi-spectral quantitative phase imaging (MS-QPI) is a cutting-edge label-free technique to determine the morphological changes, refractive index variations and spectroscopic information of the specimens. The bottleneck to implement this…

Quantitative phase imaging (QPI) has been widely applied in characterizing cells and tissues. Spatial light interference microscopy (SLIM) is a highly sensitive QPI method, due to its partially coherent illumination and common path…

Image and Video Processing · Electrical Eng. & Systems 2024-06-12 Yuheng Jiao , Yuchen R. He , Mikhail E. Kandel , Xiaojun Liu , Wenlong Lu , Gabriel Popescu

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

Quantitative phase microscopy (QPM) has found significant applications in the field of biomedical imaging which works on the principle of interferometry. The theory behind achieving interference in QPM with conventional light sources such…

Optics · Physics 2021-07-26 Azeem Ahmad , Nikhil Jayakumar , Balpreet Singh Ahluwalia

A single intensity-only holographic interferogram can records the full amplitude and phase information of optical field. However, current digital holography technologies cannot recover the lossless phase information from a single…

Image and Video Processing · Electrical Eng. & Systems 2020-03-09 Qinnan Zhang , Shengyu Lu , Jiaosheng Li , Wenjie Li , Dong Li , Xiaoxu Lu , Liyun Zhong , Jindong Tian

In this paper, we review spatial light interference microscopy (SLIM), a common-path, phase-shifting interferometer, built onto a phase-contrast microscope, with white-light illumination. As one of the most sensitive quantitative phase…

Optics · Physics 2020-12-17 Xi Chen , Mikhail E. Kandel , Gabriel Popescu

Diffusion-weighted magnetic resonance imaging (DW-MRI) can be used to characterise the microstructure of the nervous tissue, e.g. to delineate brain white matter connections in a non-invasive manner via fibre tracking. Magnetic Resonance…

Quantitative phase imaging (QPI) enables visualization and quantitative extraction of the optical phase information of transparent samples. However, conventional QPI techniques typically rely on multi-frame acquisition or complex…

In this letter, a high-fidelity single-shot differential quantitative phase microscopy (dQPM) method is presented to effectively image nearly transparent biological samples. The proposed method is based on a common-path Sagnac…

Polarization light microscopes are powerful tools for probing molecular order and orientation in birefringent materials. While a multitude of polarization light microscopy techniques are often used to access steady-state properties of…

We introduce the white light quantitative phase imaging unit (WQPIU) as a practical realization of quantitative phase imaging (QPI) on standard microscope platforms. The WQPIU is a compact stand-alone unit which measures sample induced…

Optics · Physics 2016-05-04 YoonSeok Baek , KyeoReh Lee , Jonghee Yoon , Kyoohyun Kim , YongKeun Park

Clinical adoption of multi-shot diffusion-weighted magnetic resonance imaging (multi-shot DWI) for body-wide tumor diagnostics is limited by severe motion-induced phase artifacts from respiration, peristalsis, and so on, compounded by…

This article presents a thorough comparison of themain QPM techniques, focusing on their accuracy in terms of measurement precision and trueness. We focus on 8 techniques, namely digital holographic microscopy (DHM), cross-grating wavefront…

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

Traditional phase-shifting interferometry technique cannot be used to measure time-varying phase distributions. But single shot techniques could resolve the problem. Many efforts have been made on the phase retrieval methods from a single…

Instrumentation and Detectors · Physics 2020-03-31 Lifa Hu , Wen Shena , Wenchao Ma , Dongting Hu , Xinyu Liu

Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…

Computer Vision and Pattern Recognition · Computer Science 2025-12-19 Paloma Casteleiro Costa , Parnian Ghapandar Kashani , Xuhui Liu , Alexander Chen , Ary Portes , Julien Bec , Laura Marcu , Aydogan Ozcan

Super-resolution imaging methods that combine interferometric (z) analysis with single-molecule localization microscopy (iSMLM) have achieved ultra-high 3D precision and contributed to the elucidation of important biological…

Optics · Physics 2025-02-20 Wei Wang , Zengxin Huang , Yilin Wang , Hangfeng Li , Pakorn Kanchanawong

Interferometry techniques are essential for extracting phase information from optical systems, enabling precise measurements of dispersion and highly sensitive detection of perturbations. While phase sensing offers enhanced sensitivity…

We demonstrate accurate phase measurement from low photon level interference data using a constrained optimization method that takes into account the expected redundancy in the unknown phase function. This approach is shown to have…

Quantum Physics · Physics 2015-06-23 Mandeep Singh , Kedar Khare , Anand Kumar Jha , Shashi Prabhakar , R. P. Singh

We present a high-precision temporal-spatial phase-demodulation algorithm for phase-shifting interferometry (PSI) affected by random/systematic phase-stepping errors. Laser interferometers in standard optical-shops suffer from several error…

Signal Processing · Electrical Eng. & Systems 2019-12-11 Manuel Servin , Moises Padilla , Guillermo Garnica , Gonzalo Paez
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