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Related papers: Simulating topological domains in human chromosome…

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Lamina-associated domains (LADs) cover a large part of the human genome and are thought to play a major role in shaping the nuclear architectural landscape. Here, we perform polymer simulations, microscopy and mass spectrometry to dissect…

The three dimensional structure of DNA in the nucleus (chromatin) plays an important role in many cellular processes. Recent experimental advances have led to high-throughput methods of capturing information about chromatin conformation on…

Genomics · Quantitative Biology 2015-11-05 Kevin Emmett , Benjamin Schweinhart , Raul Rabadan

Combining genome-wide structural models with phenomenological data is at the forefront of efforts to understand the organizational principles regulating the human genome. Here, we use chromosome-chromosome contact data as knowledge-based…

Biological Physics · Physics 2016-10-31 Marco Di Stefano , Jonas Paulsen , Tonje G. Lien , Eivind Hovig , Cristian Micheletti

We showed in our previous studies that just $3\%$ cross-links, at special points along the contour of the bacterial DNA help the DNA-polymer to get organized at micron length scales \cite{jpcm,epl}. In this work, we investigate how does the…

Soft Condensed Matter · Physics 2019-05-01 Tejal Agarwal , G. P. Manjunath , Farhat Habib , Apratim Chatterji

Motivated by the problem of domain formation in chromosomes, we studied a co--polymer model where only a subset of the monomers feel attractive interactions. These monomers are displaced randomly from a regularly-spaced pattern, thus…

Biomolecules · Quantitative Biology 2018-02-13 Matteo Negri , Marco Gherardi , Guido Tiana , Marco Cosentino Lagomarsino

Cellular functions crucially depend on the precise execution of complex biochemical reactions taking place on the chromatin fiber in the tightly packed environment of the cell nucleus. Despite the availability of large data sets probing…

Quantitative Methods · Quantitative Biology 2023-03-17 Alex Chen Yi Zhang , Angelo Rosa , Guido Sanguinetti

We systematically explore the self-assembly of semi-flexible polymers in deformable spherical confinement across a wide regime of chain stiffness, contour lengths and packing fractions by means of coarse-grained molecular dynamics…

Soft Condensed Matter · Physics 2021-07-12 Maxime M. C. Tortora , Daniel Jost

One of the most important problems in development is how epigenetic domains can be first established, and then maintained, within cells. To address this question, we propose a framework which couples 3D chromatin folding dynamics, to a…

Soft Condensed Matter · Physics 2016-10-25 Davide Michieletto , Enzo Orlandini , Davide Marenduzzo

Our aim is to predict how often genic and non-genic promoters fire within a cell. We first review a parsimonious pan-genomic model for genome organization and gene regulation, where transcription rate is determined by proximity in 3D space…

Biological Physics · Physics 2024-12-04 Giuseppe Negro , Massimiliano Semeraro , Perter R Cook , Davide Marenduzzo

Three-Dimensional (3D) chromatin interactions, such as enhancer-promoter interactions (EPIs), loops, Topologically Associating Domains (TADs), and A/B compartments play critical roles in a wide range of cellular processes by regulating gene…

Genomics · Quantitative Biology 2024-03-07 Brydon P. G. Wall , My Nguyen , J. Chuck Harrell , Mikhail G. Dozmorov

We compare a newly developed hybrid simulation method which combines classical molecular dynamics (MD) and computational fluid dynamics (CFD) to a simulation consisting only of molecular dynamics. The hybrid code is composed of three…

Soft Condensed Matter · Physics 2009-11-10 Sandra Barsky , Rafael Delgado-Buscalioni , Peter Coveney

In this paper we study the role of topology in DNA gel electrophoresis experiments via molecular dynamics simulations. The gel is modelled as a 3D array of obstacles from which half edges are removed at random with probability p, thereby…

Soft Condensed Matter · Physics 2015-04-10 Davide Michieletto , Marco Baiesi , Enzo Orlandini , Matthew S. Turner

Molecular and genetic definitions of human-specific changes to genomic regulatory networks (GRNs) contributing to development of unique to human phenotypes remain a highly significant challenge. Genome-wide proximity placement analysis of…

Genomics · Quantitative Biology 2025-12-30 Gennadi Glinsky

The three-dimensional organization of chromatin, on the length scale of a few genes, is crucial in determining the functional state - accessibility and the amount of gene expression - of the chromatin. Recent advances in chromosome…

Soft Condensed Matter · Physics 2021-07-16 K. Kumari , B. Duenweg , R. Padinhateeri , J. Ravi Prakash

To understand the nature of a cell, one needs to understand the structure of its genome. For this purpose, experimental techniques such as Hi-C detecting chromosomal contacts are used to probe the three-dimensional genomic structure. These…

Quantitative Methods · Quantitative Biology 2019-09-23 Andreas Hofmann , Fatema Zahra Rashid , Frédéric Crémazy , Remus T. Dame , Dieter W. Heermann

The chromosome is a key player of cell physiology, and its dynamics provides valuable information about its physical organization. In both prokaryotes and eukaryotes, the short-time motion of chromosomal loci has been described as a Rouse…

Biological Physics · Physics 2018-02-28 K. E. Polovnikov , M. Gherardi , M. Cosentino-Lagomarsino , M. V. Tamm

Chromosomes are crumpled polymer chains further folded into a sequence of stochastic loops via loop extrusion. While extrusion has been verified experimentally, the particular means by which the extruding complexes bind DNA polymer remains…

Soft Condensed Matter · Physics 2023-06-14 Kirill Polovnikov , Bogdan Slavov

The principles underlying the architectural landscape of chromatin beyond the nucleosome level in living cells remains largely unknown despite its potential to play a role in mammalian gene regulation. We investigated the 3-dimensional…

Genomics · Quantitative Biology 2012-06-27 Ivan Junier , Ryan Dale , Chunhui Hou , François Képès , Ann Dean

We analyze structural and conformational properties in a simulated bead-spring model of a non-entangled, supercooled polymer melt. We explore the statics of the model via various structure factors, involving not only the monomers, but also…

Soft Condensed Matter · Physics 2009-11-10 Martin Aichele , Song-Ho Chong , Jörg Baschnagel , Matthias Fuchs

Rotational misalignment or twisting of two mono-layers of graphene strongly influences its electronic properties. Structurally, twisting leads to large periodic supercell structures, which in turn can support intriguing strongly correlated…