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Structured illumination can reject out-of-focus signal from a sample, enabling high-speed and high-contrast imaging over large areas with widefield detection optics. Currently, this optical-sectioning technique is limited by image…

Organoids are complex, three dimensional, self-organizing cell cultures which manifest organ-like features and represent a powerful platform for studying human disease and developing treatment options. Organoid development is characterized…

Computer Vision and Pattern Recognition · Computer Science 2026-05-06 Chase Cartwright , Gongbo Guo , Sai Teja Pusuluri , Christopher N. Mayhew , Mark Hester , Horacio E. Castillo

Live cell culture is crucial in biomedical studies for analyzing cell properties and dynamics in vitro. This study focuses on segmenting unstained live cells imaged with bright-field microscopy. While many segmentation approaches exist for…

Quantitative Methods · Quantitative Biology 2025-08-26 Surajit Das , Gourav Roy , Pavel Zun

The Segment Anything Model (SAM) exhibits a capability to segment a wide array of objects in natural images, serving as a versatile perceptual tool for various downstream image segmentation tasks. In contrast, medical image segmentation…

Computer Vision and Pattern Recognition · Computer Science 2023-08-29 Yizhe Zhang , Tao Zhou , Shuo Wang , Ye Wu , Pengfei Gu , Danny Z. Chen

Identifying complex neural circuitry from electron microscopic (EM) images may help unlock the mysteries of the brain. However, identifying this circuitry requires time-consuming, manual tracing (proofreading) due to the size and intricacy…

Quantitative Methods · Quantitative Biology 2014-09-04 Stephen M. Plaza

Automated experiments in scanning transmission electron microscopy (STEM) require rapid image segmentation to optimize data representation for human interpretation, decision-making, site-selective spectroscopies, and atomic manipulation.…

Materials Science · Physics 2024-09-23 Kamyar Barakati , Utkarsh Pratiush , Austin C. Houston , Gerd Duscher , Sergei V. Kalinin

Estimation of blood oxygenation with spectroscopic photoacoustic imaging is a promising tool for several biomedical applications. For this method to be quantitative, it relies on an accurate method of the light fluence in the tissue. This…

Image and Video Processing · Electrical Eng. & Systems 2019-11-06 Geoffrey P. Luke , Kevin Hoffer-Hawlik , Austin C. Van Namen , Ruibo Shang

In the domain of battery research, the processing of high-resolution microscopy images is a challenging task, as it involves dealing with complex images and requires a prior understanding of the components involved. The utilization of deep…

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Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Determination of fundamental mechanisms of disease often hinges on histopathology visualization and quantitative image analysis. Currently, the analysis of multi-channel fluorescence tissue images is primarily achieved by manual…

Research increasingly relies on computational methods to analyze experimental data and predict molecular properties. Current approaches often require researchers to use a variety of tools for statistical analysis and machine learning,…

Quantitative Methods · Quantitative Biology 2025-12-01 Luke Rimmo Lego , Samantha Gauthier , Denver Jn. Baptiste

Optical coherence tomography angiography (OCTA) is a non-invasive imaging modality that can acquire high-resolution volumes of the retinal vasculature and aid the diagnosis of ocular, neurological and cardiac diseases. Segmenting the…

The automatic detection of disease related entities in retinal imaging data is relevant for disease- and treatment monitoring. It enables the quantitative assessment of large amounts of data and the corresponding study of disease…

Infertility is becoming an issue for an increasing number of couples. The most common solution, in vitro fertilization, requires embryologists to carefully examine light microscopy images of human oocytes to determine their developmental…

Image and Video Processing · Electrical Eng. & Systems 2022-01-10 Denis Baručić , Jan Kybic , Olga Teplá , Zinovij Topurko , Irena Kratochvílová

The wealth of data being gathered about humans and their surroundings drives new machine learning applications in various fields. Consequently, more and more often, classifiers are trained using not only numerical data but also complex data…

Machine Learning · Computer Science 2022-04-13 Maciej Piernik , Dariusz Brzezinski , Pawel Zawadzki

We propose a model-agnostic, progressive test-time energy adaptation approach for medical image segmentation. Maintaining model performance across diverse medical datasets is challenging, as distribution shifts arise from inconsistent…

Computer Vision and Pattern Recognition · Computer Science 2025-03-24 Xiaoran Zhang , Byung-Woo Hong , Hyoungseob Park , Daniel H. Pak , Anne-Marie Rickmann , Lawrence H. Staib , James S. Duncan , Alex Wong

The precise delineation of blood vessels in medical images is critical for many clinical applications, including pathology detection and surgical planning. However, fully-automated vascular segmentation is challenging because of the…

Image and Video Processing · Electrical Eng. & Systems 2024-09-20 Amine Sadikine , Bogdan Badic , Enzo Ferrante , Vincent Noblet , Pascal Ballet , Dimitris Visvikis , Pierre-Henri Conze

This study presents a machine learning-based framework for heart disease prediction using the heart-disease dataset, comprising 303 samples with 14 features. The methodology involves data preprocessing, model training, and evaluation using…

Machine Learning · Computer Science 2025-05-16 Ali Azimi Lamir , Shiva Razzagzadeh , Zeynab Rezaei

Optical-resolution photoacoustic microscopy has been validated as a high-resolution and high-sensitivity imaging modality for angiographic studies in the past decades. Quantitative vascular analysis reveals critical information of…

Medical Physics · Physics 2022-06-02 Dong Zhang , Ran Li , Xin Lou , Jianwen Luo

We introduce HAMscope, a compact, snapshot hyperspectral autofluorescence miniscope that enables real-time, label-free molecular imaging in a wide range of biological systems. By integrating a thin polymer diffuser into a widefield…