Related papers: Single-molecule orientation localization microscop…
Point Spread Function (PSF) engineering is used in single emitter localization to measure the emitter position in 3D and possibly other parameters such as the emission color or dipole orientation as well. Advanced PSF models such as spline…
We show that the position of a dark matter-wave soliton can be determined with a precision that scales with the atomic density as $n^{-3/4}$. This surpasses the standard shot-noise detection limit for independent particles, without use of…
In fluorescence microscopy, Single Molecule Localization Microscopy (SMLM) techniques aim at localizing with high precision high density fluorescent molecules by stochastically activating and imaging small subsets of blinking emitters.…
We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX minimizes the fluorescence photons needed for high localization precision. A 22-fold…
Fluoroscopic imaging that captures X-ray images at video framerates is advantageous for guiding catheter insertions by vascular surgeons and interventional radiologists. Visualizing the dynamical movements non-invasively allows complex…
We demonstrate a method for determining the three-dimensional location of single atoms in a quantum gas microscopy system using a phase-only spatial light modulator to modify the point-spread function of the high-resolution imaging system.…
Single molecule imaging is one of the main target areas of X-ray free electron lasers. It relies on the possibility of orienting the large number of low counting statistics 2D diffraction patterns taken at random orientations of identical…
Confocal microscopy in combination with real-space particle tracking has proven to be a powerful tool in scientific fields such as soft matter physics, materials science and cell biology. However, 3D tracking of anisotropic particles in…
We propose a straightforward sample-based technique to calibrate the axial detection in 3D single molecule localization microscopy (SMLM). Using microspheres coated with fluorescent molecules, the calibration curves of PSF-shaping- or…
The efficiency of an organic light-emitting diode (OLED) depends on the microscopic orientation of transition dipole moments of the molecular emitters. The most effective materials used for light generation have threefold symmetry, which…
MINFLUX microscopy allows for localization of fluorophores with nanometer precision using targeted scanning with an illumination profile with a minimum. However, current scanning patterns and the overall procedure are based on heuristics,…
Our work aims at using quantitative imaging tools to complement the limitation of noise encountered by high resolution fluorescence microscopy methods. Several cycles of fluorophore activation, imaging and deactivation produce a sequence of…
In single molecule localisation super-resolution microscopy the need for repeated image capture limits the imaging speed, while the size of fluorescence probes limits the possible theoretical localisation resolution. Here, we demonstrated a…
Light microscopy as well as image acquisition and processing suffer from physical and technical prejudices which preclude a correct interpretation of biological observations which can be reflected in, e.g., medical and pharmacological…
Considerable progress has been made in organic light-emitting diodes (OLEDs) to achieve high external quantum efficiency (EQE), among which the dipole orientation of OLED emitters has a remarkable effect. In most cases, EQE of the OLED…
3D-localization of gamma sources has the potential to improve the outcome of radio-guided surgery. The goal of this paper is to analyze the localization accuracy for point-like sources with a single coded aperture camera. We both simulated…
A variety of recent imaging techniques are able to beat the diffraction limit in fluorescence microcopy by activating and localizing subsets of the fluorescent molecules in the specimen, and repeating this process until all of the molecules…
A standard paradigm of localization microscopy involves extension from two to three dimensions by engineering information into emitter images, and approximation of errors resulting from the field dependence of optical aberrations. We invert…
The rich information content of measurements in the molecular frame rather than the laboratory frame has motivated the development of several methods for aligning gas phase molecules in space. Even so, for asymmetric tops the problem of…
We theoretically analyze the problem of detecting the position of a classical dipolar scatterer in a strongly focused optical field. We suggest an optimal measurement scheme and show that it resolves the scatterer's position in three…