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Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Diffraction limit is manifested in the loss of high spatial frequency information that results from decay of evanescent waves. As a result, conventional far-field optics yields no information about an object's subwavelength features. Here…

Optics · Physics 2012-01-24 Leonid Alekseyev , Evgenii Narimanov , Jacob Khurgin

This paper proposes a new two-step procedure for sparse-view tomographic image reconstruction. It is called RISING, since it combines an early-stopped Rapid Iterative Solver with a subsequent Iteration Network-based Gaining step. So far,…

Numerical Analysis · Mathematics 2022-01-25 Davide Evangelista , Elena Morotti , Elena Loli Piccolomini

Ptychography is an imaging technique which involves a sample being illuminated by a coherent, localized probe of illumination. When the probe interacts with the sample, the light is diffracted and a diffraction pattern is detected. Then the…

Numerical Analysis · Mathematics 2023-01-31 Mark Iwen , Michael Perlmutter , Mark Philip Roach

Magnetic Resonance Imaging (MRI), including diffusion MRI (dMRI), serves as a ``microscope'' for anatomical structures and routinely mitigates the influence of low signal-to-noise ratio scans by compromising temporal or spatial resolution.…

Image and Video Processing · Electrical Eng. & Systems 2025-03-11 Chenxu Wu , Qingpeng Kong , Zihang Jiang , S. Kevin Zhou

Quantitative phase imaging (QPI) is often used for label-free single cell analysis with its high-contrast images of optical phase delay (OPD) map. Contrary to other imaging methods, sensitivity improvement has not been intensively explored…

Optics · Physics 2025-05-06 Keiichiro Toda , Miu Tamamitsu , Takuro Ideguchi

Sub-diffraction resolution imaging has played a pivotal role in biological research by visualizing key, but previously unresolvable, sub-cellular structures. Unfortunately, applications of far-field sub-diffraction resolution are currently…

Biological Physics · Physics 2017-05-02 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

Complex field imaging, which captures both the amplitude and phase information of input optical fields or objects, can offer rich structural insights into samples, such as their absorption and refractive index distributions. However,…

Optics · Physics 2024-05-30 Jingxi Li , Yuhang Li , Tianyi Gan , Che-Yung Shen , Mona Jarrahi , Aydogan Ozcan

Information about microscopic objects with features smaller than the diffraction limit is almost entirely lost in a far-field diffraction image but could be partly recovered with data completition techniques. Any such approach critically…

Optics · Physics 2020-11-17 Maria Bancerek , Krzysztof M. Czajkowski , Rafal Kotynski

Machine learning is attracting surging interest across nearly all scientific areas by enabling the analysis of large datasets and the extraction of scientific information from incomplete data. Data-driven science is rapidly growing,…

Applied Physics · Physics 2025-03-17 Sung Yun Lee , Do Hyung Cho , Chulho Jung , Daeho Sung , Daewoong Nam , Sangsoo Kim , Changyong Song

Single-pixel imaging (SPI) is a novel technique capturing 2D images using a photodiode, instead of conventional 2D array sensors. SPI owns high signal-to-noise ratio, wide spectrum range, low cost, and robustness to light scattering.…

Computer Vision and Pattern Recognition · Computer Science 2017-12-12 Liheng Bian , Jinli Suo , Qionghai Dai , Feng Chen

Phase retrieval aims to recover a signal from intensity-only measurements, a fundamental problem in many fields such as imaging, holography, optical computing, crystallography, and microscopy. Although there are several well-known phase…

Image and Video Processing · Electrical Eng. & Systems 2026-01-21 Mehmet Onurcan Kaya , Figen S. Oktem

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

Total internal reflection fluorescence (TIRF) microscopy and its variants are key technologies for visualizing the dynamics of single molecules or organelles in live cells. Yet, truely quantitative TIRF remains problematic. One unknown…

Biological Physics · Physics 2017-06-07 Maia Brunstein , Lopamudra Roy , Martin Oheim

We introduce a compact, two-camera laparoscope that combines active stereo depth estimation and speckle-illumination spatial frequency domain imaging (si-SFDI) to map profile-corrected, pixel-level absorption and reduced scattering optical…

Optics · Physics 2025-01-27 Anthony A. Song , Mason T. Chen , Taylor L. Bobrow , Nicholas J. Durr

Traditional glass-based optics are typically optimized for narrow spectral bands, such as the visible (400-700nm) or shortwave infrared (1000-1800nm). While the emergence of VIS-SWIR sensors (400-1700nm) offers transformative potential,…

Image and Video Processing · Electrical Eng. & Systems 2026-05-04 Vishwanath Saragadam , Niki Nezakati , Amit Roy-Chowdhury , Vivek Boominathan

High resolution images can be acquired using a non-regular sampling sensor which consists of an underlying low resolution sensor that is covered with a non-regular sampling mask. The reconstructed high resolution image is then obtained…

Image and Video Processing · Electrical Eng. & Systems 2022-04-08 Markus Jonscher , Karina Jaskolka , Jürgen Seiler , André Kaup

We introduce a novel, training-free method for sampling differentiable representations (diffreps) using pretrained diffusion models. Rather than merely mode-seeking, our method achieves sampling by "pulling back" the dynamics of the…

Computer Vision and Pattern Recognition · Computer Science 2024-12-11 Yash Savani , Marc Finzi , J. Zico Kolter

While the implementation of single particle coherent diffraction imaging for non-crystalline particles is complicated by current limitations in photon flux, hit rate, and sample delivery a concept of many-particle coherent diffraction…

Disordered Systems and Neural Networks · Physics 2013-02-22 R. P. Kurta , R. Dronyak , M. Altarelli , E. Weckert , I. A. Vartanyants

Spatial Frequency Domain Imaging can map tissue scattering and absorption properties over a wide field of view, making it useful for clinical applications such as wound assessment and surgical guidance. This technique has previously…

Biological Physics · Physics 2021-02-24 Mason T. Chen , Melina Papadakis , Nicholas J. Durr
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