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In wide-field nonlinear microscopy, wavefront modulation by means of phase-only spatial light modulators (SLMs) allows achieving simultaneous two-photon excitation and fluorescence emission from specific region-of-interests (ROIs) of…

Light field cameras enable new capabilities, such as post-capture refocusing and aperture control, through capturing directional and spatial distribution of light rays in space. Micro-lens array based light field camera design is often…

Computer Vision and Pattern Recognition · Computer Science 2018-06-12 M. Umair Mukati , Bahadir K. Gunturk

Single-object imaging and spectroscopy on telescopes with apertures ranging from ~4 m to 40 m have the potential to greatly enhance the cosmological constraints that can be obtained from LSST. Two major cosmological probes will benefit…

This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae, and over 1 mm3 volumes for thick…

Optics · Physics 2019-05-22 Manish Kumar , Yevgenia Kozorovitskiy

Single-shot volumetric fluorescence (SVF) imaging offers a significant advantage over traditional imaging methods that require scanning across multiple axial planes as it can capture biological processes with high temporal resolution. The…

Conventional microscope objective lenses are diffraction limited, which means that they cannot resolve features smaller than half the illumination wavelength. Under white light illumination, such resolution limit is about 250-300 nm for an…

Instrumentation and Detectors · Physics 2021-08-26 Bing Yan , Zengbo Wang , Alan Parker , Yukun Lai , John Thomas , Liyang Yue , James Monks

3D super-resolution fluorescence microscopy typically requires sophisticated setups, sample preparation, or long measurements. A notable exception, SOFI, only requires recording a sequence of frames and no hardware modifications whatsoever…

Optics · Physics 2024-03-01 Pawel Szczypkowski , Monika Pawlowska , Radek Lapkiewicz

Single molecule localization microscopy (SMLM) techniques enable imaging biological samples well beyond the diffraction limit of light, but they vary significantly in their spatial and temporal resolutions. High-order statistical analysis…

Biological Physics · Physics 2016-03-15 Shachar Schidorsky , Xiyu Yi , Yair Razvag , Yonatan Golan , Shimon Weiss , Eilon Sherman

Confining light illumination in the three dimensions of space is a challenge for various applications. Among these, optogenetic methods developed for live experiments in cell biology would benefit from such a localized illumination as it…

Plenoptic cameras offer a cost effective solution to capture light fields by multiplexing multiple views on a single image sensor. However, the high angular resolution is achieved at the expense of reducing the spatial resolution of each…

Computer Vision and Pattern Recognition · Computer Science 2018-09-28 Reuben A. Farrugia , C. Guillemot

Camera arrays provide spatial and angular information within a single snapshot. With refocusing methods, focal planes can be altered after exposure. In this letter, we propose a light field refocusing method to improve the imaging quality…

Image and Video Processing · Electrical Eng. & Systems 2021-08-29 Yingqian Wang , Jungang Yang , Yulan Guo , Chao Xiao , Wei An

Light-sheet microscopes must compromise between field of view, optical sectioning, resolution, and detection efficiency. High-numerical-aperture (NA) detection objective lenses provide high resolution but their narrow depth of field fails…

We describe a two-beam interference structured illumination fluorescence microscope. The novelty of the presented system lies in its simplicity. A programmable electro-optical spatial light modulator in an intermediate image plane enables…

Single bubble sonoluminescence has been experimentally produced through a novel approach of optimized sound excitation. A driving consisting of a first and second harmonic with selected amplitudes and relative phase results in an increase…

chao-dyn · Physics 2009-10-31 Joachim Holzfuss , Matthias Ruggeberg , Robert Mettin

Light sheet fluorescence microscopy provides optical sectioning and is widely used in volumetric imaging of large specimens. However, the axial resolution and the lateral Field of View (FoV) of the system, defined by the light sheet,…

Optics · Physics 2021-06-16 Hongjin Li , Zihan Wu , Zhichao Yang , Karl Zhanghao , Peng Xi , Dayong Jin

Lensless optical imaging eliminates the need for refractive optics, enabling compact and low-cost cameras with a large field-of-view, supporting point-of-care diagnostics and industrial monitoring. Practical deployments, however, remain…

We propose a method for compressively acquiring a dynamic light field (a 5-D volume) through a single-shot coded image (a 2-D measurement). We designed an imaging model that synchronously applies aperture coding and pixel-wise exposure…

Image and Video Processing · Electrical Eng. & Systems 2024-11-21 Ryoya Mizuno , Keita Takahashi , Michitaka Yoshida , Chihiro Tsutake , Toshiaki Fujii , Hajime Nagahara

Light field imaging extends the traditional photography by capturing both spatial and angular distribution of light, which enables new capabilities, including post-capture refocusing, post-capture aperture control, and depth estimation from…

Computer Vision and Pattern Recognition · Computer Science 2018-03-14 M. Shahzeb Khan Gul , Bahadir K. Gunturk

Fourier ptychographic (FP) microscope is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple…

Optics · Physics 2016-03-25 Jaebum Chung , Hangwen Lu , Xiaoze Ou , Haojiang Zhou , Changhuei Yang

Single-Pixel Imaging (SPI) enables the reconstruction of objects using a single detector through sequential illuminations with structured light patterns. The choice of illumination patterns is critical, particularly in highly undersampled…

Computer Vision and Pattern Recognition · Computer Science 2026-04-28 Serban Cristian Tudosie , Alexander Denker , Zeljko Kereta , Simon Arridge