Related papers: Learning an optimal PSF-pair for ultra-dense 3D lo…
Localization microscopy is an imaging technique in which the positions of individual nanoscale point emitters (e.g. fluorescent molecules) are determined at high precision from their images. This is the key ingredient in…
Three-dimensional spatiotemporal tracking of microscopic particles in multiple colors is a challenging optical imaging task. Existing approaches require a trade-off between photon-efficiency, field of view, mechanical complexity, spectral…
Super-resolutive 3D tracking, such as PSF engineering or evanescent field imaging has long been used to track microparticles and to enhance the throughput of single molecules force spectroscopic measurements. However, current methods…
Point spread function (PSF) engineering has been pivotal in the remarkable progress made in high-resolution imaging in the last decades. However, the diversity in PSF structures attainable through existing engineering methods is limited.…
We propose a compact snapshot monocular depth estimation technique that relies on an engineered point spread function (PSF). Traditional approaches used in microscopic super-resolution imaging such as the Double-Helix PSF (DHPSF) are…
Based on point spread function (PSF) engineering and astigmatism due to a pair of cylindrical lenses, a novel compressed imaging mechanism is proposed to achieve single-shot incoherent 3D imaging. The speckle-like PSF of the imaging system…
Rotating-PSF imaging via spiral phase engineering can localize point sources over large focal depths in a snapshot mode. This letter presents a full vector-field analysis of the rotating-PSF imager that quantifies the PSF signature of the…
The past decade has brought many innovations in optical design for 3D super-resolution imaging of point-like emitters, but these methods often focus on single-emitter localization precision as a performance metric. Here, we propose a simple…
Nanoparticles (NPs) have proven their applicability in biosensing, drug delivery, and photo-thermal therapy, but their performance depends critically on the distribution and number of functional groups on their surface. When studying…
In single-molecule super-resolution microscopy, engineered point-spread functions (PSFs) are designed to efficiently encode new molecular properties, such as 3D orientation, into complex spatial features captured by a camera. To fully…
We consider the high-resolution imaging problem of 3D point source image recovery from 2D data using a method based on point spread function (PSF) engineering. The method involves a new technique, recently proposed by S.~Prasad, based on…
The point spread function (PSF) is fundamental to any type of microscopy, most importantly so for single-molecule localization techniques, where the exact PSF shape is crucial for precise molecule localization at the nanoscale. However,…
Point Spread Function (PSF) engineering is used in single emitter localization to measure the emitter position in 3D and possibly other parameters such as the emission color or dipole orientation as well. Advanced PSF models such as spline…
Nanoscale localization of point emitters is critical to several methods in optical fluorescence microscopy, including single-molecule super-resolution imaging and tracking. While the precision of the localization procedure has been the…
Among the approaches in three-dimensional (3D) single molecule localization microscopy, there are several point spread function (PSF) engineering approaches, in which depth information of molecules is encoded in 2D images. Usually,the…
Single-shot volumetric fluorescence (SVF) imaging offers a significant advantage over traditional imaging methods that require scanning across multiple axial planes as it can capture biological processes with high temporal resolution. The…
Simulated images are essential in algorithm development and instrument testing for optical telescopes. During real observations, images obtained by optical telescopes are affected by spatially variable point spread functions (PSFs), a…
When a single molecule is detected in a wide-field microscope, the image approximates the point spread function of the system. However, as the distribution of molecules becomes denser and their images begin to coincide, existing solutions…
The first step toward doing high-precision astrometry is the measurement of individual stars in individual images, a step that is fraught with dangers when the images are undersampled. The key to avoiding systematic positional error in…
Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background…