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Stochastic kinetic models of genetic expression are able to describe protein fluctuations. A comparative study of the canonical and a feedback model is given here by using stochastic simulation methods. The feedback model is skeleton model…

Molecular Networks · Quantitative Biology 2018-09-06 Raoul R. Wadhwa , Laszlo Zalanyi , Judit Szente , Laszlo Negyessy , Peter Erdi

It is well-known that gene activation/deactivation dynamics may be a major source of randomness in genetic networks, also in the case of large concentrations of the transcription factors. In this work, we investigate the effect of realistic…

Molecular Networks · Quantitative Biology 2014-10-28 Sebastiano de Franciscis , Giulio Caravagna , Alberto d'Onofrio

It has recently been suggested that the competition for a finite pool of microRNAs (miRNA) gives rise to effective interactions among their common targets (competing endogenous RNAs or ceRNAs) that could prove to be crucial for…

Molecular Networks · Quantitative Biology 2015-06-11 Matteo Figliuzzi , Enzo Marinari , Andrea De Martino

The 5' UTR, a regulatory region at the beginning of an mRNA molecule, plays a crucial role in regulating the translation process and impacts the protein expression level. Language models have showcased their effectiveness in decoding the…

Machine Learning · Computer Science 2023-10-09 Yanyi Chu , Dan Yu , Yupeng Li , Kaixuan Huang , Yue Shen , Le Cong , Jason Zhang , Mengdi Wang

Inherent stochasticity in gene expression leads to distributions of mRNA copy numbers in a population of identical cells. These distributions are determined primarily by the multitude of states of a gene promoter, each driving transcription…

Molecular Networks · Quantitative Biology 2020-06-16 Jaroslav Albert

A stochastic theory is developed to predict the spectral signature of proton-transfer processes and applied to infrared spectra computed from ab initio molecular-dynamics simulations of a single H$_5$O$_2{}^{+}$ cation. By constraining the…

Chemical Physics · Physics 2022-11-23 Florian N. Brünig , Paul Hillmann , Won Kyu Kim , Jan O. Daldrop , Roland R. Netz

We present a theoretical framework to analyze the dynamics of gene expression with stochastic bursts. Beginning with an individual-based model which fully accounts for the messenger RNA (mRNA) and protein populations, we propose a novel…

Molecular Networks · Quantitative Biology 2016-03-23 Yen Ting Lin , Charles R. Doering

Cell phenotype dynamic homeostasis contrasts with the inherent randomness of intracellular reactions. Although feedback control of master regulatory genes (MRG) is a key strategy for maintaining gene network expression ranges limited,…

Molecular Networks · Quantitative Biology 2025-08-13 Guilherme Giovanini , Cyro von Zuben de Valega Negrão , Ammar Alsinai , Alexandre Ferreira Ramos

Cell size varies between different cell types, and between different growth and osmotic conditions. However, the nuclear-to-cell volume ratio (N/C ratio) remains nearly constant. In this paper, we build on existing deterministic models of…

Cell Behavior · Quantitative Biology 2025-10-29 Xuesong Bai , Thomas G. Fai

In early development, regulation of transcription results in precisely positioned and highly reproducible expression patterns that specify cellular identities. How transcription, a fundamentally noisy molecular process, is regulated to…

Subcellular Processes · Quantitative Biology 2018-01-10 Benjamin Zoller , Shawn C. Little , Thomas Gregor

In genetic circuits, when the mRNA lifetime is short compared to the cell cycle, proteins are produced in geometrically-distributed bursts, which greatly affects the cellular switching dynamics between different metastable phenotypic…

Statistical Mechanics · Physics 2016-05-25 Shay Be'er , Metar Heller-Algazi , Michael Assaf

Regulatory genes called small RNAs (sRNAs) are known to play critical roles in cellular responses to changing environments. For several sRNAs, regulation is effected by coupled stoichiometric degradation with messenger RNAs (mRNAs). The…

Biological Physics · Physics 2015-05-27 Thierry Platini , Tao Jia , Rahul V. Kulkarni

The ribosome is a macromolecular complex which is responsible for protein synthesis in all living cells according to their transcribed genetic information. Using X-ray crystallography and, more recently, cryo-electron microscopy (cryo-EM),…

Biological Physics · Physics 2017-11-17 Lars V. Bock , Michal H. Kolář , Helmut Grubmüller

This work applies the methods of signal processing and the concepts of control system design to model the maintenance and modulation of reading frame in the process of protein synthesis. The model shows how translational speed can modulate…

Genomics · Quantitative Biology 2007-12-14 Lalit Ponnala , Donald Bitzer , Anne Stomp , Mladen Vouk

We propose a generalization of the random matrix theory following the basic prescription of the recently suggested concept of superstatistics. Spectral characteristics of systems with mixed regular-chaotic dynamics are expressed as weighted…

Statistical Mechanics · Physics 2007-05-23 A. Y. Abul-Magd

Cell-to-cell variability is inherent to numerous biological processes, including cell migration. Quantifying and characterizing the variability of migrating cells is challenging, as it requires monitoring many cells for long time windows…

Biological Physics · Physics 2021-05-06 David B. Brückner , Alexandra Fink , Joachim O. Rädler , Chase P. Broedersz

In a general stochastic multistate promoter model of dynamic mRNA/protein interactions, we identify the stationary joint distribution of the promoter state, mRNA, and protein levels through an explicit `stick-breaking' construction of…

Statistics Theory · Mathematics 2021-08-26 William Lippitt , Sunder Sethuraman , Xueying Tang

In biophysics, the search for analytical solutions of stochastic models of cellular processes is often a challenging task. In recent work on models of gene expression, it was shown that a mapping based on partitioning of Poisson arrivals…

Biological Physics · Physics 2018-05-09 Hugo Tschirhart , Thierry Platini

mRNA technology has revolutionized vaccine development, protein replacement therapies, and cancer immunotherapies, offering rapid production and precise control over sequence and efficacy. However, the inherent instability of mRNA poses…

Biomolecules · Quantitative Biology 2025-03-26 Max Ward , Mary Richardson , Mihir Metkar

Non-coding RNA sequences play a great role in controlling a number of cellular functions, thus raising the need to understand their complex conformational dynamics in quantitative detail. In this perspective, we first show that single…

Biomolecules · Quantitative Biology 2012-11-29 Jong-Chin Lin , Changbong Hyeon , D. Thirumalai
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