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Motor-proteins are responsible for transport inside cells. Harnessing their activity is key towards developing new nano-technologies, or functional biomaterials. Cytoskeleton-like networks, recently tailored in vitro, result from the…

Soft Condensed Matter · Physics 2016-06-21 Pau Guillamat , Jordi Ignés-Mullol , Francesc Sagués

Observations of single epidermal cells on flat adhesive substrates have revealed two distinct morphological and functional states, namely a non-migrating symmetric unpolarized state and a migrating asymmetric polarized state. These states…

Biological Physics · Physics 2010-04-14 Wolfgang Alt , Martin Bock , Christoph Möhl

The kinetics of the assembly of semiflexible filaments through end-to-end annealing is key to the structure of the cytoskeleton, but is not understood. We analyze this problem through scaling theory and simulations, and uncover a regime…

Soft Condensed Matter · Physics 2023-12-18 Valerio Sorichetti , Martin Lenz

Cytoskeletal crowding plays a key role in the diffusion of DNA molecules through the cell, acting as a barrier to effective intracellular transport and conformational stability required for such processes as transfection, viral infection,…

Contraction of the cytokinetic ring during cell division leads to physical partitioning of a eukaryotic cell into two daughter cells. This involves flows of actin filaments and myosin motors in the growing membrane interface at the…

Biological Physics · Physics 2022-02-21 Mainak Chatterjee , Arkya Chatterjee , Amitabha Nandi , Anirban Sain

Active fluids have applications in micromixing, but little is known about the mixing kinematics of systems with spatiotemporally-varying activity. To investigate, UV-activated caged ATP was used to activate controlled regions of…

Soft Condensed Matter · Physics 2022-11-07 Teagan E Bate , Megan E Varney , Ezra H Taylor , Joshua H Dickie , Chih-Che Chueh , Michael M Norton , Kun-Ta Wu

The mitotic spindle lies at the heart of the spatio-temporal control over cellular components during cell division. The spindle consists of microtubules, which are not only crosslinked by motor proteins but also by passive binding proteins.…

Biological Physics · Physics 2020-08-19 Harmen Wierenga , Pieter Rein ten Wolde

The growth of actin filament networks is a fundamental biological process that drives a variety of cellular and intracellular motions. During motility, eukaryotic cells and intracellular pathogens are propelled by actin networks organized…

Biological Physics · Physics 2009-11-13 Joshua W. Shaevitz , Daniel A. Fletcher

This paper describes an investigation into part of the mechanical mechanisms underlying the formation of mitotic spindle, the cellular machinery responsible for chromosomal separation during cell division. In normal eukaryotic cells,…

Subcellular Processes · Quantitative Biology 2019-05-29 Samira Masoudi , Cameron H. G. Wright , Jesse C. Gatlin , John. S. Oakey

Many processes in eukaryotic cells, including cell motility, rely on the growth of branched actin networks from surfaces. Despite its central role the mechano-chemical coupling mechanisms which guide the growth process are poorly…

Soft Condensed Matter · Physics 2015-06-23 Karin John , Denis Caillerie , Chaouqi Misbah

Amoeboid cell migration is characterized by frequent changes of the direction of motion and resembles a persistent random walk on long time scales. Although it is well known that cell migration is typically driven by the actin cytoskeleton,…

Biological Physics · Physics 2021-06-09 Nicolas Ecker , Karsten Kruse

We investigate the mechanical interplay between the spatial organization of the actin cytoskeleton and the shape of animal cells adhering on micropillar arrays. Using a combination of analytical work, computer simulations and in vitro…

The cytoskeleton -- a composite network of biopolymers, molecular motors, and associated binding proteins -- is a paradigmatic example of active matter. Particle transport through the cytoskeleton can range from anomalous and heterogeneous…

Microtubules are biological protein polymers with critical and diverse functions. Their structures share some similarities with photosynthetic antenna complexes, particularly in the ordered arrangement of photoactive molecules with large…

Biological Physics · Physics 2019-02-20 G. L. Celardo , M. Angeli , P. Kurian , T. J. A. Craddock

Cortical actin networks are highly dynamic and play critical roles in shaping the mechanical properties of cells. The actin cytoskeleton undergoes significant reorganization over the course of the cell cycle, when cortical actin transitions…

Quantitative Methods · Quantitative Biology 2022-07-22 Maria-Veronica Ciocanel , Aravind Chandrasekaran , Carli Mager , Qin Ni , Garegin Papoian , Adriana Dawes

In many organisms, cell division is driven by the constriction of a cytokinetic ring, which consists of actin filaments and crosslinking proteins. While it has long been believed that the constriction is driven by motor proteins, it has…

Biological Physics · Physics 2023-07-26 Alexander Cumberworth , Pieter Rein ten Wolde

The actin cortex is a thin layer of actin, myosin, and actin binding proteins that underlies the membrane of most animal cells. It is highly dynamic and can undergo remodelling on time-scales of tens of seconds thanks to protein turnover…

Cell Behavior · Quantitative Biology 2020-06-24 Manasi Kelkar , Pierre Bohec , Guillaume Charras

The ability of cells to move through small spaces depends on the mechanical properties of the cellular cytoskeleton and on nuclear deformability. In mammalian cells, the cytoskeleton is comprised of three interacting, semi-flexible polymer…

Biological Physics · Physics 2021-10-27 Sarthak Gupta , Alison E. Patteson , J. M. Schwarz

The polarization and motility of eukaryotic cells depends on assembly and contraction of the actin cytoskeleton and its regulation by proteins called GTPases. The activity of GTPases causes assembly of filamentous actin (by GTPases Cdc42,…

Cell Behavior · Quantitative Biology 2021-10-18 Yue Liu , Elisabeth G. Rens , Leah Edelstein-Keshet

Actin filaments, crosslinkers and myosin molecular motors form contractile networks. For instance, the cell cortex is a thin network below the cell membrane ; contraction of the cell cortex allows cells to round up during cell division.…