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Histopathology, particularly hematoxylin and eosin (H\&E) staining, plays a critical role in diagnosing and characterizing pathological conditions by highlighting tissue morphology. However, H\&E-stained images inherently lack molecular…

Computational Engineering, Finance, and Science · Computer Science 2025-01-28 Qing Wang , Wen-jie Chen , Bo Li , Jing Su , Guangyu Wang , Qianqian Song

Immunohistochemical (IHC) staining provides crucial molecular characterization of tissue samples and plays an indispensable role in the clinical examination and diagnosis of cancers. However, compared with the commonly used Hematoxylin and…

Computer Vision and Pattern Recognition · Computer Science 2026-01-07 Mingzhou Jiang , Jiaying Zhou , Nan Zeng , Mickael Li , Qijie Tang , Chao He , Huazhu Fu , Honghui He

The current study detects different morphologies related to prostate pathology using deep learning models; these models were evaluated on 2,121 hematoxylin and eosin (H&E) stain histology images captured using bright field microscopy, which…

Large amounts of digitized histopathological data display a promising future for developing pathological foundation models via self-supervised learning methods. Foundation models pretrained with these methods serve as a good basis for…

Computer Vision and Pattern Recognition · Computer Science 2024-08-07 Shengyi Hua , Fang Yan , Tianle Shen , Lei Ma , Xiaofan Zhang

Universal, transferable whole-slide image (WSI) representations are central to computational pathology. Incorporating multiple markers (e.g., immunohistochemistry, IHC) alongside H&E enriches H&E-based features with diverse, biologically…

Computer Vision and Pattern Recognition · Computer Science 2025-12-04 Yizhi Zhang , Lei Fan , Zhulin Tao , Donglin Di , Yang Song , Sidong Liu , Cong Cong

Variations in hematoxylin and eosin (H&E) stained images (due to clinical lab protocols, scanners, etc) directly impact the quality and accuracy of clinical diagnosis, and hence it is important to control for these variations for a reliable…

Image and Video Processing · Electrical Eng. & Systems 2020-06-26 Saad Nadeem , Travis Hollmann , Allen Tannenbaum

Organ transplantation serves as the primary therapeutic strategy for end-stage organ failures. However, allograft rejection is a common complication of organ transplantation. Histological assessment is essential for the timely detection and…

While multiplex immunofluorescence (mIF) imaging provides deep, spatially-resolved molecular data, integrating this information with the morphological standard of Hematoxylin & Eosin (H&E) can be very important for obtaining complementary…

Quantitative Methods · Quantitative Biology 2025-08-08 Jillur Rahman Saurav , Mohammad Sadegh Nasr , Jacob M. Luber

Histopathology relies on the analysis of microscopic tissue images to diagnose disease. A crucial part of tissue preparation is staining whereby a dye is used to make the salient tissue components more distinguishable. However, differences…

Image and Video Processing · Electrical Eng. & Systems 2022-08-03 Haseeb Nazki , Ognjen Arandjelović , InHwa Um , David Harrison

Performance of deep learning algorithms decreases drastically if the data distributions of the training and testing sets are different. Due to variations in staining protocols, reagent brands, and habits of technicians, color variation in…

Computer Vision and Pattern Recognition · Computer Science 2020-12-01 Abhijeet Patil , Mohd. Talha , Aniket Bhatia , Nikhil Cherian Kurian , Sammed Mangale , Sunil Patel , Amit Sethi

Modern histopathology relies on the microscopic examination of thin tissue sections stained with histochemical techniques, typically using brightfield or fluorescence microscopy. However, the staining of samples can permanently alter their…

Virtual stain transfer is a promising area of research in Computational Pathology, which has a great potential to alleviate important limitations when applying deeplearningbased solutions such as lack of annotations and sensitivity to a…

Computer Vision and Pattern Recognition · Computer Science 2022-10-19 Jelica Vasiljević , Friedrich Feuerhake , Cédric Wemmert , Thomas Lampert

In the present study, we propose a novel case-based similar image retrieval (SIR) method for hematoxylin and eosin (H&E)-stained histopathological images of malignant lymphoma. When a whole slide image (WSI) is used as an input query, it is…

Manual counting of mitotic tumor cells in tissue sections constitutes one of the strongest prognostic markers for breast cancer. This procedure, however, is time-consuming and error-prone. We developed a method to automatically detect…

The current standard for detecting human epidermal growth factor receptor 2 (HER2) status in breast cancer patients relies on HER2 amplification, identified through fluorescence in situ hybridization (FISH) or immunohistochemistry (IHC).…

Image and Video Processing · Electrical Eng. & Systems 2024-09-27 Ardhendu Sekhar , Vrinda Goel , Garima Jain , Abhijeet Patil , Ravi Kant Gupta , Tripti Bameta , Swapnil Rane , Amit Sethi

An optical microscopic examination of thinly cut stained tissue on glass slides prepared from a FFPE tissue blocks is the gold standard for tissue diagnostics. In addition, the diagnostic abilities and expertise of any pathologist is…

Breast cancer presents a significant healthcare challenge globally, demanding precise diagnostics and effective treatment strategies, where histopathological examination of Hematoxylin and Eosin (H&E) stained tissue sections plays a central…

Image and Video Processing · Electrical Eng. & Systems 2024-08-06 Linhao Qu , Chengsheng Zhang , Guihui Li , Haiyong Zheng , Chen Peng , Wei He

Hematoxylin and Eosin (H&E) staining is a widely used sample preparation procedure for enhancing the saturation of tissue sections and the contrast between nuclei and cytoplasm in histology images for medical diagnostics. However, various…

Image and Video Processing · Electrical Eng. & Systems 2023-07-18 Fangda Li , Zhiqiang Hu , Wen Chen , Avinash Kak

With the advent of digital scanners and deep learning, diagnostic operations may move from a microscope to a desktop. Hematoxylin and Eosin (H&E) staining is one of the most frequently used stains for disease analysis, diagnosis, and…

Computer Vision and Pattern Recognition · Computer Science 2023-08-28 Shikha Dubey , Tushar Kataria , Beatrice Knudsen , Shireen Y. Elhabian

Breast Cancer is a major cause of death worldwide among women. Hematoxylin and Eosin (H&E) stained breast tissue samples from biopsies are observed under microscopes for the primary diagnosis of breast cancer. In this paper, we propose a…

Computer Vision and Pattern Recognition · Computer Science 2018-07-26 Aditya Golatkar , Deepak Anand , Amit Sethi