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A long-standing challenge in multiple-particle-tracking is the accurate and precise 3D localization of individual particles at close proximity. One established approach for snapshot 3D imaging is point-spread-function (PSF) engineering, in…

Image and Video Processing · Electrical Eng. & Systems 2020-10-01 Elias Nehme , Boris Ferdman , Lucien E. Weiss , Tal Naor , Daniel Freedman , Tomer Michaeli , Yoav Shechtman

Localization microscopy is an imaging technique in which the positions of individual nanoscale point emitters (e.g. fluorescent molecules) are determined at high precision from their images. This is the key ingredient in…

Image and Video Processing · Electrical Eng. & Systems 2020-10-01 Elias Nehme , Daniel Freedman , Racheli Gordon , Boris Ferdman , Lucien E. Weiss , Onit Alalouf , Reut Orange , Tomer Michaeli , Yoav Shechtman

Three-dimensional spatiotemporal tracking of microscopic particles in multiple colors is a challenging optical imaging task. Existing approaches require a trade-off between photon-efficiency, field of view, mechanical complexity, spectral…

Interferometric scattering (iSCAT) microscopy is currently among the most powerful techniques available for achieving high-sensitivity single-particle localization. This capability is realized through homodyne detection, where interference…

Optics · Physics 2024-10-08 Nathan J. Brooks , Chih-Chen Liu , Chia-Lung Hsieh

The point spread function (PSF) is fundamental to any type of microscopy, most importantly so for single-molecule localization techniques, where the exact PSF shape is crucial for precise molecule localization at the nanoscale. However,…

Single-molecule detection enables direct observation of individual biomolecular events, providing mechanistic insights into biological processes and offering a powerful tool for disease diagnostics. However, the fundamental scale mismatch…

Optics · Physics 2025-10-14 Guifeng Li , Chaoyang Gong

Recently, several single-pixel imaging (SPI) schemes have emerged for imaging fast-moving objects and have shown dramatic results. However, fast image reconstruction of a moving object with high quality is still challenging for SPI, thereby…

Optics · Physics 2024-10-08 Shijian Li , Xu-Ri Yao , Wei Zhang , Yeliang Wang , Qing Zhao

One of the key problems in 3D object detection is to reduce the accuracy gap between methods based on LiDAR sensors and those based on monocular cameras. A recently proposed framework for monocular 3D detection based on Pseudo-Stereo has…

Computer Vision and Pattern Recognition · Computer Science 2023-07-06 Yuguang Shi

We introduce a compact, two-camera laparoscope that combines active stereo depth estimation and speckle-illumination spatial frequency domain imaging (si-SFDI) to map profile-corrected, pixel-level absorption and reduced scattering optical…

Optics · Physics 2025-01-27 Anthony A. Song , Mason T. Chen , Taylor L. Bobrow , Nicholas J. Durr

The technique of simultaneous spectral differential imaging (SSDI) is used to image directly brown dwarfs and exoplanets around nearby stars. An analytical PSF model is first presented with numerical simulations to estimate the PSF noise…

Astrophysics · Physics 2007-05-23 C. Marois

Single-shot volumetric fluorescence (SVF) imaging offers a significant advantage over traditional imaging methods that require scanning across multiple axial planes as it can capture biological processes with high temporal resolution. The…

In Real-Time, Feedback-Driven Single Particle Tracking methods, measurements of the emission intensity from a labeled, nanometer-scale particle are used in a feedback loop to track the motion of the particle as it moves inside its native…

Optics · Physics 2025-12-03 Md Faysal Hossain , Sean B. Andersson

This paper introduces Spectral Incoherent Diffractive Imaging (SIDI) as a novel method for achieving dark-field imaging of nanostructures with heterogeneous oxidation states. With SIDI, shifts in photoemission profiles can be spatially…

Optics · Physics 2023-12-25 Tamme Wollweber , Kartik Ayyer

Complex-field imaging is indispensable for numerous applications at wavelengths from X-ray to THz, with amplitude describing transmittance (or reflectivity) and phase revealing intrinsic structure of the target object. Coherent diffraction…

Image and Video Processing · Electrical Eng. & Systems 2021-04-07 Meng Li , Liheng Bian , Guoan Zheng , Andrew Maiden , Yang Liu , Yiming Li , Qionghai Dai , Jun Zhang

Point-spread-function (PSF) engineering is a well-established computational imaging technique that uses phase masks and other optical elements to embed extra information (e.g., depth) into the images captured by conventional CMOS image…

Computer Vision and Pattern Recognition · Computer Science 2024-06-14 Sachin Shah , Matthew Albert Chan , Haoming Cai , Jingxi Chen , Sakshum Kulshrestha , Chahat Deep Singh , Yiannis Aloimonos , Christopher Metzler

Interferometric scattering (iSCAT) microscopy is an emerging label-free technique optimized for the sensitive detection of nano-matter. Previous iSCAT studies have approximated the point spread function in iSCAT by a Gaussian intensity…

3D single-pixel imaging (SPI) is a promising imaging technique that can be ffexibly applied to various wavebands. The main challenge in 3D SPI is that the calibration usually requires a large number of standard points as references, which…

Image and Video Processing · Electrical Eng. & Systems 2024-10-11 Xinyue Ma , Chenxing Wang

Tracking moving masses in several degrees of freedom with high precision and large dynamic range is a central aspect in many current and future gravitational physics experiments. Laser interferometers have been established as one of the…

Scattering-based single-particle tracking (S-SPT) has opened new avenues for highly sensitive label-free detection and characterization of nanoscopic objects, making it particularly attractive for various analytical applications. However, a…

We propose a compact snapshot monocular depth estimation technique that relies on an engineered point spread function (PSF). Traditional approaches used in microscopic super-resolution imaging such as the Double-Helix PSF (DHPSF) are…

Image and Video Processing · Electrical Eng. & Systems 2022-08-05 Bhargav Ghanekar , Vishwanath Saragadam , Dushyant Mehra , Anna-Karin Gustavsson , Aswin Sankaranarayanan , Ashok Veeraraghavan
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