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Among the approaches in three-dimensional (3D) single molecule localization microscopy, there are several point spread function (PSF) engineering approaches, in which depth information of molecules is encoded in 2D images. Usually,the…

Optics · Physics 2024-07-11 Hanzhe Wu , Danni Chen , YiHong Jiand Gan Xiang , Heng Li , Bin Yu , JunLe Qu

Single-molecule localization microscopy (SMLM) allows reconstructing biology-relevant structures beyond the diffraction limit by detecting and localizing individual fluorophores -- fluorescent molecules stained onto the observed specimen --…

Computer Vision and Pattern Recognition · Computer Science 2026-03-03 Romain Seailles , Jean-Baptiste Masson , Jean Ponce , Julien Mairal

Fundamental understanding of biological pathways requires minimally invasive nanoscopic optical resolution imaging. Many approaches to high-resolution imaging rely on localization of single emitters, such as fluorescent molecule or quantum…

Quantum Physics · Physics 2022-04-05 Shuo Li , Wenchao Li , Vladislav V. Yakovlev , Allison Kealy , Andrew D. Greentree

The short and intense pulses of the new X-ray free electron lasers, now operational or under construction, may make possible diffraction experiments on single molecule-sized objects with high resolution, before radiation damage destroys the…

Data Analysis, Statistics and Probability · Physics 2012-04-11 Miklós Tegze , Gábor Bortel

Single particle-resolved fluorescence imaging is an enabling technology in cold-atom physics. However, so far, this technique was not available for nanophotonic atom-light interfaces. Here, we image single atoms that are trapped and…

Polarization imaging can yield crucial information in multiple applications of remote sensing, such as characterization of clouds, aerosols, and the Aurora Borealis. Some applications require sub-percent polarimetric sensitivity and…

Instrumentation and Methods for Astrophysics · Physics 2024-05-14 Thijs Stockmans , Naor Scheinowitz , Erwoud van der Linden , Irina Malysheva , Kira Strelow , Martijn Smit , Frans Snik

We present high resolution two-photon excitation microscopy studies combining two-photon fluorescence (TPF) and second harmonic generation (SHG) in order to probe orientational distributions of molecular ensembles at room temperature. A…

Soft Condensed Matter · Physics 2007-05-23 Veronique Le Floc'h , Sophie Brasselet , Jean-Francois Roch , Joseph Zyss

Colocalization is a powerful tool to study the interactions between fluorescently labeled molecules in biological fluorescence microscopy. However, existing techniques for colocalization analysis have not undergone continued development…

Methodology · Statistics 2018-02-14 Shulei Wang , Ellen T. Arena , Kevin W. Eliceiri , Ming Yuan

Controlling quantum light-matter interactions at scales smaller than the diffraction limit at the single quantum emitter level is a critical challenge to the goal of advancing quantum technologies. We introduce a novel material platform…

Optical antennas have been extensively employed to manipulate the photophysical properties of single photon emitters. Coupling between an emitter and a given resonant mode of an optical antenna depends mainly on three parameters: spectral…

In the past decade superconducting nanowire single photon detectors (SNSPDs) have gradually become an indispensable part of any demanding quantum optics experiment. Until now, most SNSPDs are coupled to single-mode fibers. SNSPDs coupled to…

Measuring subdiffraction separations between single fluorescent particles is important for biological, nano-, and medical-technology studies. Major challenges include (i) measuring changing molecular separations with high temporal…

Biological Physics · Physics 2015-05-18 Shawn H. DeCenzo , Michael C. DeSantis , Y. M. Wang

Characterizing and manipulating the circular polarization of light is central to numerous emerging technologies, including spintronics and quantum computing. Separately, monolayer tungsten disulfide (WS2) is a versatile material that has…

Live cell imaging using metallic nanoparticles as tags is an emerging technique to visualize long and highly dynamic processes due to the lack of photobleaching and high photon rate. However, the lack of excited states as compared to…

Mesoscale and Nanoscale Physics · Physics 2011-01-12 H. E. Grecco , O. E. Martínez

Optical superresolution microscopy is an important field, where nonlinear optical processes or prior information is used to defeat the classical diffraction limit of light. Quantum correlation microscopy uses photon arrival statistics from…

Optics · Physics 2023-10-13 Jaret J. Vasquez-Lozano , Shuo Li , Andrew D. Greentree

Anderson localization of light is a fundamental emergent phenomenon in disordered systems. In arrays of coupled waveguides, it suppresses transport and causes photons to remain localized near the excitation site as coupling disorder…

Localized polymerization in subwavelength volumes using two photon dyes has now become a well-established method for fabrication of subwavelength structures. Unfortunately, the two photon absorption dyes used in such process are not only…

Soft Condensed Matter · Physics 2016-09-09 Govind Ummethala , Arun Jaiswal , Raghvendra P Chaudhary , Suyog Hawal , Sumit Saxena , Shobha Shukla

We use a single trapped 40Ca+ ion as a resonant, polarization-sensitive absorber to detect and characterize the entanglement of tunable narrowband photon pairs from a spontaneous parametric down-conversion source. Single-photon absorption…

Quantum Physics · Physics 2013-04-22 J. Huwer , J. Ghosh , N. Piro , M. Schug , F. Dubin , J. Eschner

This paper is concerned with investigating super-resolution algorithms and solutions for handling electron microscopic images. We note two main aspects differentiating the problem discussed here from those considered in the literature. The…

Image and Video Processing · Electrical Eng. & Systems 2020-08-26 Yanjun Qian , Jiaxi Xu , Lawrence F. Drummy , Yu Ding

We present experimental results on a method to perform polarimetry on ensembles of single photons. Our setup is based on a measurement method known to be optimal for estimating the state of two level systems. The setup has no moving parts…

Quantum Physics · Physics 2009-11-11 Alexander Ling , Soh Kee Pang , Antia Lamas-Linares , Christian Kurtsiefer