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Cellular membrane dynamics play an important role in a variety of physiological processes. However, due to the stringent light-coupling conditions required for exciting evanescent waves, label-free mapping of cellular membrane dynamics on…

Biological Physics · Physics 2024-10-22 Chaoyang Gong , Yu-Cheng Chen

Automated semantic segmentation of cell nuclei in microscopic images is crucial for disease diagnosis and tissue microenvironment analysis. Nonetheless, this task presents challenges due to the complexity and heterogeneity of cells. While…

Image and Video Processing · Electrical Eng. & Systems 2023-08-10 Zhuchen Shao , Sourya Sengupta , Hua Li , Mark A. Anastasio

We propose a general framework for a collaborative machine learning system to assist bioscience researchers with the task of labeling specific cell identities from microscopic still or video imaging. The distinguishing features of this…

Quantitative Methods · Quantitative Biology 2019-03-25 Greg Bubnis , Steven Ban , Matthew D. DiFranco , Saul Kato

Despite fluorescent cell-labelling being widely employed in biomedical studies, some of its drawbacks are inevitable, with unsuitable fluorescent probes or probes inducing a functional change being the main limitations. Consequently, the…

Quantitative Methods · Quantitative Biology 2020-03-03 Yan Ge , Philipp Rosendahl , Claudio Durán , Nicole Töpfner , Sara Ciucci , Jochen Guck , Carlo Vittorio Cannistraci

The automated analysis of microscopy images is a challenge in the context of single-cell tracking and quantification. This work has as goals the study of the performance of deep learning for segmenting microscopy images and the improvement…

Quantitative Methods · Quantitative Biology 2022-10-05 André O. Françani

Hyperspectral imaging is a rich source of data, allowing for multitude of effective applications. However, such imaging remains challenging because of large data dimension and, typically, small pool of available training examples. While…

Neural and Evolutionary Computing · Computer Science 2020-10-23 Wojciech Masarczyk , Przemysław Głomb , Bartosz Grabowski , Mateusz Ostaszewski

Progress in neuroscience constantly relies on the development of new techniques to investigate the complex dynamics of neuronal networks. An ongoing challenge is to achieve minimally-invasive and high-resolution observations of neuronal…

Semantic segmentation of microscopy images is a critical task for high-throughput materials characterisation, yet its automation is severely constrained by the prohibitive cost, subjectivity, and scarcity of expert-annotated data. While…

Computer Vision and Pattern Recognition · Computer Science 2026-02-03 Salma Zahran , Zhou Ao , Zhengyang Zhang , Chen Chi , Chenchen Yuan , Yanming Wang

Semantic segmentation of neuronal structures in 3D high-resolution fluorescence microscopy imaging of the human brain cortex can take advantage of bidimensional CNNs, which yield good results in neuron localization but lead to inaccurate…

Image and Video Processing · Electrical Eng. & Systems 2020-09-03 Filippo Maria Castelli , Matteo Roffilli , Giacomo Mazzamuto , Irene Costantini , Ludovico Silvestri , Francesco Saverio Pavone

Brightfield microscopy of unstained live cells is challenging due to low contrast, dynamic morphology, uneven illumination, and lack of labels. Deep learning achieved SOTA performance on stained, high-contrast images but needs large labeled…

Image and Video Processing · Electrical Eng. & Systems 2025-10-15 Surajit Das , Pavel Zun

Cell segmentation is essential in biomedical research for analyzing cellular morphology and behavior. Deep learning methods, particularly convolutional neural networks (CNNs), have revolutionized cell segmentation by extracting intricate…

Image and Video Processing · Electrical Eng. & Systems 2024-08-27 Boyuan Peng , Jiaju Chen , P. Bilha Githinji , Ijaz Gul , Qihui Ye , Minjiang Chen , Peiwu Qin , Xingru Huang , Chenggang Yan , Dongmei Yu , Jiansong Ji , Zhenglin Chen

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Volumetric cell segmentation in fluorescence microscopy images is important to study a wide variety of cellular processes. Applications range from the analysis of cancer cells to behavioral studies of cells in the embryonic stage. Like in…

Image and Video Processing · Electrical Eng. & Systems 2022-11-29 Royden Wagner , Karl Rohr

The success of supervised deep learning models on cell recognition tasks relies on detailed annotations. Many previous works have managed to reduce the dependency on labels. However, considering the large number of cells contained in a…

Computer Vision and Pattern Recognition · Computer Science 2023-08-23 Pingyi Chen , Chenglu Zhu , Zhongyi Shui , Jiatong Cai , Sunyi Zheng , Shichuan Zhang , Lin Yang

Quantitative analysis of cell structures is essential for biomedical and pharmaceutical research. The standard imaging approach relies on fluorescence microscopy, where cell structures of interest are labeled by chemical staining…

The advancement of the neuroscientific imaging techniques has produced an unprecedented size of neural cell imaging data, which calls for automated processing. In particular, identification of cells from two photon images demands…

Image and Video Processing · Electrical Eng. & Systems 2019-09-26 Si-Baek Seong , Hae-Jeong Park

Existing image segmentation networks mainly leverage large-scale labeled datasets to attain high accuracy. However, labeling medical images is very expensive since it requires sophisticated expert knowledge. Thus, it is more desirable to…

Image and Video Processing · Electrical Eng. & Systems 2021-02-04 Yuhang Ding , Xin Yu , Yi Yang

Biomedical image segmentation is critical for precise structure delineation and downstream analysis. Traditional methods often struggle with noisy data, while deep learning models such as U-Net have set new benchmarks in segmentation…

Computer Vision and Pattern Recognition · Computer Science 2025-11-10 Shuo Zhao , Yu Zhou , Jianxu Chen

Fluorescent Neuronal Cells v2 is a collection of fluorescence microscopy images and the corresponding ground-truth annotations, designed to foster innovative research in the domains of Life Sciences and Deep Learning. This dataset…

Generally, microscopy image analysis in biology relies on the segmentation of individual nuclei, using a dedicated stained image, to identify individual cells. However stained nuclei have drawbacks like the need for sample preparation, and…

Image and Video Processing · Electrical Eng. & Systems 2022-11-08 Gendarme Mathieu , Lambert Annika M. , El Debs Bachir