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I explain what is, what is not, and what is only sort of superresolution microscopy. I discuss optical resolution, first in terms of diffraction theory, then in terms of linear systems theory, and finally in terms of techniques that use…

Optics · Physics 2015-04-02 John Bechhoefer

Speckles arise when coherent light interacts with biological tissues. Information retrieval from speckles is desired yet challenging, requiring understanding or mapping of the multiple scattering process, or reliable capability to reverse…

Image and Video Processing · Electrical Eng. & Systems 2020-05-05 Huanhao Li , Zhipeng Yu , Yunqi Luo , Shengfu Cheng , Lihong V. Wang , Yuanjin Zheng , Puxiang Lai

The inherent non-linearity of intensity correlation functions can be used to spatially distinguish identical emitters beyond the diffraction limit, as achieved, for example, in Super-Resolution Optical Fluctuation Imaging (SOFI). Here, we…

Optics · Physics 2024-12-18 Yifan Chen , Chieh Tsao , Hendrik Utzat

The optical diffraction limit, formulated by Abbe 140 years ago, imposes a bound on imaging resolution in classical optics. Over the last twenty years, many theoretical schemes have been presented for overcoming the diffraction barrier in…

Quantum Physics · Physics 2013-07-12 O. Schwartz , J. M. Levitt , R. Tenne , S. Itzhakov , Z. Deutsch , D. Oron

Phase-sensitive coherent imaging exploits changes in the phases of backscattered light to observe tiny alterations of scattering structures or variations of the refractive index. But moving scatterers or a fluctuating refractive index…

Encoding of spectral information onto monochrome imaging cameras is of interest for wavelength multiplexing and hyperspectral imaging applications. Here, the complex spatio-spectral response of a disordered material is used to demonstrate…

Optics · Physics 2017-05-09 Rebecca French , Sylvain Gigan , Otto L. Muskens

In fluorescence microscopy, spectral unmixing aims to recover individual fluorophore concentrations from spectral images that capture mixed fluorophore emissions. Since classical methods operate pixel-wise and rely on least-squares fitting,…

Computer Vision and Pattern Recognition · Computer Science 2026-03-26 Federico Carrara , Talley Lambert , Mehdi Seifi , Florian Jug

Metasurfaces have attracted extensive interests due to their ability to locally manipulate optical parameters of light and easy integration to complex optical systems. Particularly, metasurfaces can provide a novel platform for splitting…

Confocal microscopy has long been a cornerstone technique for visualizing complex interactions and processes within cellular structures. However, achieving super-resolution imaging of multiple organelles and their interactions…

Optics · Physics 2025-08-19 Qinglin Chen , Luwei Wang , Jia Li , Dan Shao , Xiaoyu Weng , Liwei Liu , Dayong Jin , Junle Qu

Ultrasound imaging is widely used for real-time, noninvasive diagnosis, but speckle and related artifacts reduce image quality and can hinder interpretation. We present a diffusion-based ultrasound despeckling method built on the Image…

Computer Vision and Pattern Recognition · Computer Science 2026-02-27 Shuoqi Chen , Yujia Wu , Geoffrey P. Luke

Illumination of colloid sphere monolayers by circularly polarized beams enables the fabrication of concave patterns consisting of circular nanohole miniarrays that can be transferred into convex metal nanoparticle patterns via a lift-off…

Optics · Physics 2020-05-21 Aron Sipos , Emese Toth , Oliver A. Fekete , Maria Csete

We propose a novel method to perform plenoptic imaging at the diffraction limit by measuring second-order correlations of light between two reference planes, arbitrarily chosen, within the tridimensional scene of interest. We show that for…

Speckle-based sensing exploits the rich environmental information of its high-dimensional spatial intensity patterns. However, the requirement for camera-based acquisition and subsequent electronic digitization introduces significant…

Hyperspectral target detection is a pixel-level recognition problem. Given a few target samples, it aims to identify the specific target pixels such as airplane, vehicle, ship, from the entire hyperspectral image. In general, the background…

Computer Vision and Pattern Recognition · Computer Science 2021-05-11 Can Yao , Yuan Yuan , Zhiyu Jiang

Nonlinear structured illumination microscopy (nSIM) is an effective approach for super-resolution wide-field fluorescence microscopy with a theoretically unlimited resolution. In nSIM, carefully designed, highly-contrasted illumination…

The resolution of optical imaging devices is ultimately limited by the diffraction of light. To circumvent this limit, modern super-resolution microscopy techniques employ active interaction with the object by exploiting its optical…

Optics · Physics 2022-01-05 A. A. Pushkina , G. Maltese , J. I. Costa-Filho , P. Patel , A. I. Lvovsky

Several approaches have been proposed to solve the spectral unmixing problem in hyperspectral image analysis. Among them the use of sparse regression techniques aims to characterize the abundances in pixels based on a large library of…

Image and Video Processing · Electrical Eng. & Systems 2021-02-12 L. C. Ayres , S. J. M. de Almeida , J. C. M. Bermudez , R. A. Borsoi

The emerging optical multiplexing within nanoscale shows super-capacity in encoding information by using the time-domain fingerprints from uniform nanoparticles. However, the optical diffraction limit compromises the decoding throughput and…

Optics · Physics 2022-08-09 Baolei Liu , Jiayan Liao , Yiliao Song , Jie Lu , Jiajia Zhou , Fan Wang

Optical spectroscopy plays an essential role across scientific research and industry for non-contact materials analysis1-3, increasingly through in-situ or portable platforms4-6. However, when considering low-light-level applications,…

Recently, imaging by microspheres and dielectric particle-lenses emerged as a simple solution to obtaining super-resolution images of nanoscale devices and structures. Calibrated resolution of ~{\lambda}/6 - {\lambda}/8 has been…

Optics · Physics 2021-08-26 Zengbo Wang , Boris Luk'yanchuk