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Optical aberrations prevent telescopes from reaching their theoretical diffraction limit. Once estimated, these aberrations can be compensated for using deformable mirrors in a closed loop. Focal plane wavefront sensing enables the…

Quantitative phase imaging (QPI) is a label-free technique providing both morphology and quantitative biophysical information in biomedicine. However, applying such a powerful technique to in vivo pathological diagnosis remains challenging.…

We present a simple and effective method to eliminate system aberrations and speckle noise in quantitative phase imaging. Using spiral integration, complete information about system aberration is calculated from three laterally shifted…

Biological Physics · Physics 2018-02-14 Inhyeok Choi , Kyeoreh Lee , YongKeun Park

Purpose: Quantitative phase imaging (QPI) is a label-free technique that provides high-contrast images of tissues and cells without the use of chemicals or dyes. Accurate semantic segmentation of cells in QPI is essential for various…

Image and Video Processing · Electrical Eng. & Systems 2024-05-13 Zhuchen Shao , Mark A. Anastasio , Hua Li

Machine learning is attracting surging interest across nearly all scientific areas by enabling the analysis of large datasets and the extraction of scientific information from incomplete data. Data-driven science is rapidly growing,…

Applied Physics · Physics 2025-03-17 Sung Yun Lee , Do Hyung Cho , Chulho Jung , Daeho Sung , Daewoong Nam , Sangsoo Kim , Changyong Song

Estimation of optical aberrations from volumetric intensity images is a key step in sensorless adaptive optics for 3D microscopy. Recent approaches based on deep learning promise accurate results at fast processing speeds. However,…

Image and Video Processing · Electrical Eng. & Systems 2020-10-28 Debayan Saha , Uwe Schmidt , Qinrong Zhang , Aurelien Barbotin , Qi Hu , Na Ji , Martin J. Booth , Martin Weigert , Eugene W. Myers

In the field of quantitative imaging, the image information at a pixel or voxel in an underlying domain entails crucial information about the imaged matter. This is particularly important in medical imaging applications, such as…

Optimization and Control · Mathematics 2024-04-12 Guozhi Dong , Moritz Flaschel , Michael Hintermüller , Kostas Papafitsoros , Clemens Sirotenko , Karsten Tabelow

Quantitative phase imaging (QPI) recovers the exact wavefront of light from the intensity measured by a camera. Topographical maps of translucent microscopic bodies can be extracted from these quantified phase shifts. We demonstrate…

With applications ranging from metabolomics to histopathology, quantitative phase microscopy (QPM) is a powerful label-free imaging modality. Despite significant advances in fast multiplexed imaging sensors and deep-learning-based inverse…

Computational image reconstruction algorithms generally produce a single image without any measure of uncertainty or confidence. Regularized Maximum Likelihood (RML) and feed-forward deep learning approaches for inverse problems typically…

Machine Learning · Computer Science 2020-12-18 He Sun , Katherine L. Bouman

We introduce the white light quantitative phase imaging unit (WQPIU) as a practical realization of quantitative phase imaging (QPI) on standard microscope platforms. The WQPIU is a compact stand-alone unit which measures sample induced…

Optics · Physics 2016-05-04 YoonSeok Baek , KyeoReh Lee , Jonghee Yoon , Kyoohyun Kim , YongKeun Park

The image blurring process is generally modelled as the convolution of a blur kernel with a latent image. Therefore, the estimation of the blur kernel is essentially important for blind image deblurring. Unlike existing approaches which…

Computer Vision and Pattern Recognition · Computer Science 2019-04-18 Liyuan Pan , Richard Hartley , Miaomiao Liu , Yuchao Dai

Optical imaging quality can be severely degraded by system and sample induced aberrations. Existing adaptive optics systems typically rely on iterative search algorithm to correct for aberrations and improve images. This study demonstrates…

In Fourier ptychography, multiple low resolution images are captured and subsequently combined computationally into a high-resolution, large-field of view micrograph. A theoretical image-formation model based on the assumption of plane-wave…

Optics · Physics 2022-06-22 Tomas Aidukas , Lars Loetgering , Andrew Robert Harvey

Using a deep neural network, we demonstrate a digital staining technique, which we term PhaseStain, to transform quantitative phase images (QPI) of labelfree tissue sections into images that are equivalent to brightfield microscopy images…

Image and Video Processing · Electrical Eng. & Systems 2019-02-08 Yair Rivenson , Tairan Liu , Zhensong Wei , Yibo Zhang , Aydogan Ozcan

Due to its specificity, fluorescence microscopy (FM) has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit FM's utility. Recently, it has been shown that…

In coherent X-ray diffraction microscopy the diffraction pattern generated by a sample illuminated with coherent x-rays is recorded, and a computer algorithm recovers the unmeasured phases to synthesize an image. By avoiding the use of a…

Quantitative MRI (qMRI) refers to a class of MRI methods for quantifying the spatial distribution of biological tissue parameters. Traditional qMRI methods usually deal separately with artifacts arising from accelerated data acquisition,…

Image and Video Processing · Electrical Eng. & Systems 2025-02-21 Xiaojian Xu , Weijie Gan , Satya V. V. N. Kothapalli , Dmitriy A. Yablonskiy , Ulugbek S. Kamilov

Phase retrieval, the problem of recovering lost phase information from measured intensity alone, is an inverse problem that is widely faced in various imaging modalities ranging from astronomy to nanoscale imaging. The current process of…

Image and Video Processing · Electrical Eng. & Systems 2024-06-12 Henry Chan , Youssef S. G. Nashed , Saugat Kandel , Stephan Hruszkewycz , Subramanian Sankaranarayanan , Ross J. Harder , Mathew J. Cherukara

We introduce a data-free quantization method for deep neural networks that does not require fine-tuning or hyperparameter selection. It achieves near-original model performance on common computer vision architectures and tasks. 8-bit…

Machine Learning · Computer Science 2019-11-26 Markus Nagel , Mart van Baalen , Tijmen Blankevoort , Max Welling