Related papers: Holographic immunoassays
We present a cost-effective, simple and fast digital holographic microscopy method based upon Rayleigh-Sommerfeld back propagation for identification of the geometrical shape of a cell. The method was tested using synthetic hologram images…
In recent years nanoscale coherent imaging has emerged as an indispensable imaging modality allowing to surpass the resolution limit given by classical imaging optics. At the same time, attosecond science has experienced enormous progress…
The major histocompatibility complex (MHC) molecules, which bind peptides for presentation on the cell surface, play an important role in cell-mediated immunity. In light of developing databases and technologies over the years, significant…
The presence of atomic gas mixed with molecular species in a "molecular" cloud may significantly affect its chemistry, the excitation of some species, and can serve as probe of the cloud's evolution. Cold neutral atomic hydrogen (HI) in…
In-line holographic video microscopy records a wealth of information about the microscopic structure and dynamics of colloidal materials. Powerful analytical techniques are available to retrieve that information when the colloidal particles…
Plasmonic metamaterials can be used to enhance mid-infrared sensors and detectors for immunosensing applications. The major challenge is to integrate these technologies in low-cost, compact, and promising platforms. In this study, as an…
Holotomography, a three-dimensional quantitative phase imaging technique, presents an innovative, non-invasive approach to studying biological samples by exploiting the refractive index as an intrinsic imaging contrast. Despite offering…
In this chapter, we introduce digital holographic microscopy (DHM) as a marker-free method to determine the refractive index of single, spherical cells in suspension. The refractive index is a conclusive measure in a biological context.…
Since holography yields exact results, even in situations where perturbation theory is not applicable, it is an ideal framework for modeling strongly correlated systems. We extend previous holographic methods to take the dynamical charge…
Growth hormone (GH) constitutes a set of closely related protein isoforms. In clinical practice, the disagreement of test results between commercially available ligand-binding assays is still an ongoing issue, and incomplete knowledge about…
We utilize a multiscale modeling framework to study the effect of shape, size and ligand composition on the efficacy of binding of a ligand-coated-particle to a substrate functionalized with the target receptors. First, we show how…
The development of compact indirect microwave holographic set-up by the implementation of low cost, specifically designed directive antennae as transmitter and receiver is proposed. Microwave holograms are recorded by 2D scanning over a…
Aggregation-based assays, using micro- and nano-particles have been widely accepted as an efficient and cost-effective bio-sensing tool, particularly in microbiology, where particle clustering events are used as a metric to infer the…
We present a coarse-grained model for evaluation of interactions of globular proteins with nanoparticles. The protein molecules are represented by one bead per aminoacid and the nanoparticle by a homogeneous sphere that interacts with the…
One of the molecular properties most intuitive to the human perception is the geometrical shape. However, when exploring a large chemical space the determination of shape needs to be automated. We present a fast and simple approach to…
The paper presents a hybrid bubble hologram processing approach for measuring the size and 3D distribution of bubbles over a wide range of size and shape. The proposed method consists of five major steps, including image enhancement,…
In recent years, cell-based assays have been frequently used in molecular interaction analysis. Cell-based assays complement traditional biochemical and biophysical methods, as they allow for molecular interaction analysis, mode of action…
Despite its potential for label-free particle diagnostics, holographic microscopy is limited by specialized processing methods that struggle to generalize across diverse settings. We introduce a deep learning architecture leveraging human…
A lateral shear interferometer based digital holographic microscopy has been realized to study the morphology dynamics of Human red blood cells quantitatively. Here, a lateral shear interferometer is embedded with a conventional microscope…
Enzyme-Linked ImmunoSorbent Assays (ELISA) are a range of widely used analytical methods whose implementation requires to build antibodies (IgG) thin films onto surfaces predominantly made of polystyrene. The high hydrophobicity of…