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Calcium imaging is a technique for observing neuron activity as a series of images showing indicator fluorescence over time. Manually segmenting neurons is time-consuming, leading to research on automated calcium imaging segmentation…

Computer Vision and Pattern Recognition · Computer Science 2017-07-21 Aleksander Klibisz , Derek Rose , Matthew Eicholtz , Jay Blundon , Stanislav Zakharenko

Microscopy images are powerful tools and widely used in the majority of research areas, such as biology, chemistry, physics and materials fields by various microscopies (scanning electron microscope (SEM), atomic force microscope (AFM) and…

Computer Vision and Pattern Recognition · Computer Science 2020-02-25 Israel Goytom , Qin Wang , Tianxiang Yu , Kunjie Dai , Kris Sankaran , Xinfei Zhou , Dongdong Lin

Volumetric imaging of samples using fluorescence microscopy plays an important role in various fields including physical, medical and life sciences. Here we report a deep learning-based volumetric image inference framework that uses 2D…

Optics · Physics 2021-03-24 Luzhe Huang , Yilin Luo , Yair Rivenson , Aydogan Ozcan

Computational imaging modalities support a simplification of the active architectures required in an imaging system and these approaches have been validated across the electromagnetic spectrum. Recent implementations have utilized…

Classical Physics · Physics 2016-08-24 Thomas Fromenteze , Xiaojun Liu , Michael Boyarsky , Jonah Gollub , David R. Smith

Fourier Ptychographic Microscopy (FPM) is a computational technique that achieves a large space-bandwidth product imaging. It addresses the challenge of balancing a large field of view and high resolution by fusing information from multiple…

Optics · Physics 2023-04-21 Ruiqing Sun , Delong Yang , Yao Hu , Qun Hao , Xin Li , Shaohui Zhang

Deep Neural Networks are increasingly used in video frame interpolation tasks such as frame rate changes as well as generating fake face videos. Our project aims to apply recent advances in Deep video interpolation to increase the temporal…

Image and Video Processing · Electrical Eng. & Systems 2020-05-15 Rohit Saha , Abenezer Teklemariam , Ian Hsu , Alan M. Moses

Deep-neural-network-based image reconstruction has demonstrated promising performance in medical imaging for under-sampled and low-dose scenarios. However, it requires large amount of memory and extensive time for the training. It is…

Computer Vision and Pattern Recognition · Computer Science 2019-06-12 Dufan Wu , Kyungsang Kim , Quanzheng Li

Counting cells in fluorescent microscopy is a tedious, time-consuming task that researchers have to accomplish to assess the effects of different experimental conditions on biological structures of interest. Although such objects are…

Computer Vision and Pattern Recognition · Computer Science 2021-03-02 R. Morelli , L. Clissa , M. Dalla , M. Luppi , L. Rinaldi , A. Zoccoli

Tracking cells in 3D at high speed continues to attract extensive attention for many biomedical applications, such as monitoring immune cell migration and observing tumor metastasis in flowing blood vessels. Here, we propose a deep…

Optics · Physics 2018-05-16 Kan Liu , Hui Qiao , Jiamin Wu , Haoqian Wang , Lu Fang , Qionghai Dai

Fluorescence microscopic imaging in centimeter-deep tissue has been highly sought-after for many years because much interesting in vivo micro-information, such as microcirculation, tumor angiogenesis, and metastasis, may deeply locate in…

Flow-Imaging Microscopy (FIM) is commonly used in both academia and industry to characterize subvisible particles (those $\le 25 \mu m$ in size) in protein therapeutics. Pharmaceutical companies are required to record vast volumes of FIM…

Quantitative Methods · Quantitative Biology 2017-09-04 Christopher P. Calderon , Austin L. Daniels , Theodore W. Randolph

Confocal laser endomicroscopy (CLE) is an advanced optical fluorescence technology undergoing assessment for applications in brain tumor surgery. Despite its promising potential, interpreting the unfamiliar gray tone images of fluorescent…

Computer Vision and Pattern Recognition · Computer Science 2018-08-21 Mohammadhassan Izadyyazdanabadi , Evgenii Belykh , Michael Mooney , Nikolay Martirosyan , Jennifer Eschbacher , Peter Nakaji , Mark C. Preul , Yezhou Yang

Imaging through optical multimode fibers (MMFs) has the potential to enable hair-thin endoscopes that reduce the invasiveness of imaging deep inside tissues and organs. Current approaches predominantly require active wavefront shaping and…

Optics · Physics 2021-12-13 Szu-Yu Lee , Vicente J. Parot , Brett E. Bouma , Martin Villiger

Images are an important data source for diagnosis and treatment of oral diseases. The manual classification of images may lead to misdiagnosis or mistreatment due to subjective errors. In this paper an image classification model based on…

Computer Vision and Pattern Recognition · Computer Science 2017-05-26 Sultan Imangaliyev , Monique H. van der Veen , Catherine M. C. Volgenant , Bruno G. Loos , Bart J. F. Keijser , Wim Crielaard , Evgeni Levin

Cannulation for hemodialysis is the act of inserting a needle into a surgically created vascular access (e.g., an arteriovenous fistula) for the purpose of dialysis. The main risk associated with cannulation is infiltration, the puncture of…

Image and Video Processing · Electrical Eng. & Systems 2021-10-25 Jianxin Gao , Ju Lin , Irfan Kil , Ravikiran B. Singapogu , Richard E. Groff

We demonstrate that in situ coherent diffractive imaging (CDI), which harnesses the coherent interference between a strong and a weak beam illuminating a static and dynamic structure, can be a very dose-efficient imaging method. At low…

Deep learning algorithms offer a powerful means to automatically analyze the content of medical images. However, many biological samples of interest are primarily transparent to visible light and contain features that are difficult to…

Computer Vision and Pattern Recognition · Computer Science 2017-09-22 Roarke Horstmeyer , Richard Y. Chen , Barbara Kappes , Benjamin Judkewitz

We demonstrate that a deep neural network can significantly improve optical microscopy, enhancing its spatial resolution over a large field-of-view and depth-of-field. After its training, the only input to this network is an image acquired…

Machine Learning · Computer Science 2017-11-21 Yair Rivenson , Zoltan Gorocs , Harun Gunaydin , Yibo Zhang , Hongda Wang , Aydogan Ozcan

In neuroscience, all kinds of computation models were designed to answer the open question of how sensory stimuli are encoded by neurons and conversely, how sensory stimuli can be decoded from neuronal activities. Especially, functional…

Computer Vision and Pattern Recognition · Computer Science 2018-01-03 Kai Qiao , Chi Zhang , Linyuan Wang , Bin Yan , Jian Chen , Lei Zeng , Li Tong

Today the gold standard for in vivo imaging through scattering tissue is the point-scanning two-photon microscope (PSTPM). Especially in neuroscience, PSTPM is widely used for deep-tissue imaging in the brain. However, due to sequential…

Image and Video Processing · Electrical Eng. & Systems 2020-01-03 Zhun Wei , Josiah R. Boivin , Yi Xue , Xudong Chen , Peter T. C. So , Elly Nedivi , Dushan N. Wadduwage