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Reconstructing a synaptic wiring diagram, or connectome, from electron microscopy (EM) images of brain tissue currently requires many hours of manual annotation or proofreading (Kasthuri and Lichtman, 2010; Lichtman and Sanes, 2008; Seung,…

Classifying structural variability in noisy projections of biological macromolecules is a central problem in Cryo-EM. In this work, we build on a previous method for estimating the covariance matrix of the three-dimensional structure…

Computer Vision and Pattern Recognition · Computer Science 2015-12-08 Joakim Andén , Eugene Katsevich , Amit Singer

The Cryo-EM 3D particle reconstruction is essential for identifying protein and uncover the biological mechanism of the macro-molecules. In this paper, we use Kam method for reconstruction. Kam method is \textit{ab-initio}, and it assumes…

Quantitative Methods · Quantitative Biology 2019-06-21 Yin Xian

Object segmentation and structure localization are important steps in automated image analysis pipelines for microscopy images. We present a convolution neural network (CNN) based deep learning architecture for segmentation of objects in…

Computer Vision and Pattern Recognition · Computer Science 2019-01-24 Shan E Ahmed Raza , Linda Cheung , Muhammad Shaban , Simon Graham , David Epstein , Stella Pelengaris , Michael Khan , Nasir M. Rajpoot

Efforts to automate the reconstruction of neural circuits from 3D electron microscopic (EM) brain images are critical for the field of connectomics. An important computation for reconstruction is the detection of neuronal boundaries. Images…

Computer Vision and Pattern Recognition · Computer Science 2015-08-21 Kisuk Lee , Aleksandar Zlateski , Ashwin Vishwanathan , H. Sebastian Seung

Single particle reconstruction (SPR) from cryo-electron microscopy (EM) is a technique in which the 3D structure of a molecule needs to be determined from its contrast transfer function (CTF) affected, noisy 2D projection images taken at…

Applications · Statistics 2017-06-30 Tejal Bhamre , Zhizhen Zhao , Amit Singer

Quantification of microstructures is crucial for understanding processing-structure and structure-property relationships in polycrystalline materials. Delineating grain boundaries in bright-field transmission electron micrographs, however,…

We introduce ICE-TIDE, a method for cryogenic electron tomography (cryo-ET) that simultaneously aligns observations and reconstructs a high-resolution volume. The alignment of tilt series in cryo-ET is a major problem limiting the…

Image and Video Processing · Electrical Eng. & Systems 2024-12-16 Valentin Debarnot , Vinith Kishore , Ricardo D. Righetto , Ivan Dokmanić

Deep learning based image denoising methods have been extensively investigated. In this paper, attention mechanism enhanced kernel prediction networks (AME-KPNs) are proposed for burst image denoising, in which, nearly cost-free attention…

Image and Video Processing · Electrical Eng. & Systems 2020-01-30 Bin Zhang , Shenyao Jin , Yili Xia , Yongming Huang , Zixiang Xiong

Detecting synaptic clefts is a crucial step to investigate the biological function of synapses. The volume electron microscopy (EM) allows the identification of synaptic clefts by photoing EM images with high resolution and fine details.…

Computer Vision and Pattern Recognition · Computer Science 2021-01-13 Yi Liu , Shuiwang Ji

Automatic cell segmentation is an essential step in the pipeline of computer-aided diagnosis (CAD), such as the detection and grading of breast cancer. Accurate segmentation of cells can not only assist the pathologists to make a more…

Image and Video Processing · Electrical Eng. & Systems 2020-12-29 Muyi Sun , Zeyi Yao , Guanhong Zhang

With the development of deep learning, medical image processing has been widely used to assist clinical research. This paper focuses on the denoising problem of low-dose computed tomography using deep learning. Although low-dose computed…

Image and Video Processing · Electrical Eng. & Systems 2026-05-19 Zhilin Guan , Wei Zhang

Cryo-electron microscopy is a technique in structural biology for discovering/determining the 3D structure of small molecules. A key step in this process is detecting common lines of intersection between unknown embedded image planes. We…

Algebraic Geometry · Mathematics 2014-02-25 David Dynerman

Structural dynamics of macromolecules is critical to their structural-function relationship. Cryogenic electron microscopy (CryoEM) provides snapshots of vitrified protein at different compositional and conformational states, and the…

Quantitative Methods · Quantitative Biology 2026-01-27 Muyuan Chen , Muchen Li , Renjie Liao

Diffusion Models (DMs) have exhibited superior performance in generating high-quality and diverse images. However, this exceptional performance comes at the cost of expensive architectural design, particularly due to the attention module…

Computer Vision and Pattern Recognition · Computer Science 2024-05-09 Hongjie Wang , Difan Liu , Yan Kang , Yijun Li , Zhe Lin , Niraj K. Jha , Yuchen Liu

Multi-scale 3D characterization is widely used by materials scientists to further their understanding of the relationships between microscopic structure and macroscopic function. Scientific computed tomography (CT) instruments are one of…

Image and Video Processing · Electrical Eng. & Systems 2022-01-12 S. V. Venkatakrishnan , K. Aditya Mohan , Amir Koushyar Ziabari , Charles A. Bouman

Three-dimensional (3D) reconstruction of head Computed Tomography (CT) images elucidates the intricate spatial relationships of tissue structures, thereby assisting in accurate diagnosis. Nonetheless, securing an optimal head CT scan…

Computer Vision and Pattern Recognition · Computer Science 2023-09-18 Bowen Zheng , Chenxi Huang , Yuemei Luo

Existing edge-aware camouflaged object detection (COD) methods normally output the edge prediction in the early stage. However, edges are important and fundamental factors in the following segmentation task. Due to the high visual…

Computer Vision and Pattern Recognition · Computer Science 2023-07-11 Dongyue Sun , Shiyao Jiang , Lin Qi

Microfluidic devices offer numerous advantages in medical applications, including the capture of single cells in microwell-based platforms for genomic analysis. As the cost of sequencing decreases, the demand for high-throughput single-cell…

Computational Engineering, Finance, and Science · Computer Science 2024-09-13 Xueying Zhao , Yan Chen , Yuefu Jiang , Amie Radenbaugh , Jamie Moskwa , Devon Jensen

One of the difficulties in 3D reconstruction of molecules from images in single particle Cryo-Electron Microscopy (Cryo-EM), in addition to high levels of noise and unknown image orientations, is heterogeneity in samples: in many cases, the…

Computer Vision and Pattern Recognition · Computer Science 2016-07-13 Roy R. Lederman , Amit Singer
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