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Purpose: In digital histopathology, virtual multi-staining is important for diagnosis and biomarker research. Additionally, it provides accurate ground-truth for various deep-learning tasks. Virtual multi-staining can be obtained using…

Image and Video Processing · Electrical Eng. & Systems 2023-12-15 Johannes Lotz , Nick Weiss , Jeroen van der Laak , Stefan Heldmann

Histological examination is a crucial step in an autopsy; however, the traditional histochemical staining of post-mortem samples faces multiple challenges, including the inferior staining quality due to autolysis caused by delayed fixation…

Image registration refers to the process of spatially aligning two or more images by mapping them into a common coordinate system, so that corresponding anatomical or tissue structures are matched across images. In digital pathology,…

Computer Vision and Pattern Recognition · Computer Science 2025-11-07 Fatemehzahra Darzi , Rodrigo Escobar Diaz Guerrero , Thomas Bocklitz

Variability in staining protocols, such as different slide preparation techniques, chemicals, and scanner configurations, can result in a diverse set of whole slide images (WSIs). This distribution shift can negatively impact the…

Image and Video Processing · Electrical Eng. & Systems 2023-04-25 Kudaibergen Abutalip , Numan Saeed , Mustaqeem Khan , Abdulmotaleb El Saddik

The analysis of FFPE tissue sections stained with haematoxylin and eosin (H&E) or immunohistochemistry (IHC) is an essential part of the pathologic assessment of surgically resected breast cancer specimens. IHC staining has been broadly…

Stain normalization algorithms aim to transform the color and intensity characteristics of a source multi-gigapixel histology image to match those of a target image, mitigating inconsistencies in the appearance of stains used to highlight…

Image and Video Processing · Electrical Eng. & Systems 2024-07-15 Robert Jewsbury , Ruoyu Wang , Abhir Bhalerao , Nasir Rajpoot , Quoc Dang Vu

Early diagnosis of the cancer cells is necessary for making an effective treatment plan and for the health and safety of a patient. Nowadays, doctors usually use a histological grade that pathologists determine by performing a…

Computer Vision and Pattern Recognition · Computer Science 2023-11-15 Varan Singh Rohila , Neeraj Lalwani , Lochan Basyal

In this paper we propose to perform model ensembling in a multiclass or a multilabel learning setting using Wasserstein (W.) barycenters. Optimal transport metrics, such as the Wasserstein distance, allow incorporating semantic side…

Machine Learning · Computer Science 2019-02-14 Pierre Dognin , Igor Melnyk , Youssef Mroueh , Jerret Ross , Cicero Dos Santos , Tom Sercu

Deploying deep learning-based imaging tools across various clinical sites poses significant challenges due to inherent domain shifts and regulatory hurdles associated with site-specific fine-tuning. For histopathology, stain normalization…

Wasserstein Barycenter is a principled approach to represent the weighted mean of a given set of probability distributions, utilizing the geometry induced by optimal transport. In this work, we present a novel scalable algorithm to…

Machine Learning · Computer Science 2021-11-30 Jiaojiao Fan , Amirhossein Taghvaei , Yongxin Chen

The popular use of histopathology images, such as hematoxylin and eosin (H&E), has proven to be useful in detecting tumors. However, moving such cancer cases forward for treatment requires accurate on the amount of the human epidermal…

Computer Vision and Pattern Recognition · Computer Science 2025-12-30 Olaide N. Oyelade , Oliver Hoxey , Yulia Humrye

Immunohistochemical (IHC) staining highlights the molecular information critical to diagnostics in tissue samples. However, compared to H&E staining, IHC staining can be much more expensive in terms of both labor and the laboratory…

Computer Vision and Pattern Recognition · Computer Science 2023-03-14 Fangda Li , Zhiqiang Hu , Wen Chen , Avinash Kak

Hyperspectral unmixing is a blind source separation problem which consists in estimating the reference spectral signatures contained in a hyperspectral image, as well as their relative contribution to each pixel according to a given mixture…

Data Analysis, Statistics and Probability · Physics 2017-11-21 Pierre-Antoine Thouvenin , Nicolas Dobigeon , Jean-Yves Tourneret

Optimal transport is a notoriously difficult problem to solve numerically, with current approaches often remaining intractable for very large scale applications such as those encountered in machine learning. Wasserstein barycenters -- the…

Machine Learning · Computer Science 2021-02-25 Julien Lacombe , Julie Digne , Nicolas Courty , Nicolas Bonneel

While human observers are able to cope with variations in color and appearance of histological stains, digital pathology algorithms commonly require a well-normalized setting to achieve peak performance, especially when a limited amount of…

Computer Vision and Pattern Recognition · Computer Science 2017-08-15 Daniel Bug , Steffen Schneider , Anne Grote , Eva Oswald , Friedrich Feuerhake , Julia Schüler , Dorit Merhof

In histology, the presence of collagen in the extra-cellular matrix has both diagnostic and prognostic value for cancer malignancy, and can be highlighted by adding Saffron (S) to a routine Hematoxylin and Eosin (HE) staining. However,…

Image and Video Processing · Electrical Eng. & Systems 2024-10-28 Guillaume Balezo , Christof A. Bertram , Cyprien Tilmant , Stéphanie Petit , Saima Ben Hadj , Rutger H. J. Fick

We present new algorithms to compute the mean of a set of empirical probability measures under the optimal transport metric. This mean, known as the Wasserstein barycenter, is the measure that minimizes the sum of its Wasserstein distances…

Machine Learning · Statistics 2014-06-18 Marco Cuturi , Arnaud Doucet

Due to its superior efficiency in utilizing annotations and addressing gigapixel-sized images, multiple instance learning (MIL) has shown great promise as a framework for whole slide image (WSI) classification in digital pathology…

Quantitative Methods · Quantitative Biology 2023-07-14 Qiehe Sun , Jiawen Li , Jin Xu , Junru Cheng , Tian Guan , Yonghong He

Spatial Transcriptomics (ST) allows a high-resolution measurement of RNA sequence abundance by systematically connecting cell morphology depicted in Hematoxylin and Eosin (H&E) stained histology images to spatially resolved gene…

Computer Vision and Pattern Recognition · Computer Science 2025-03-21 Sichen Zhu , Yuchen Zhu , Molei Tao , Peng Qiu

Staining reveals the micro structure of the aspirate while creating histopathology slides. Stain variation, defined as a chromatic difference between the source and the target, is caused by varying characteristics during staining, resulting…

Image and Video Processing · Electrical Eng. & Systems 2022-08-31 Nilanjan Chattopadhyay , Shiv Gehlot , Nitin Singhal
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