Related papers: Interpreting Holographic Molecular Binding Assays …
An in-line hologram of a colloidal sphere can be analyzed with the Lorenz-Mie theory of light scattering to measure the sphere's three-dimensional position with nanometer-scale precision while also measuring its diameter and refractive…
Holographic molecular binding assays detect macromolecules binding to colloidal probe beads by monitoring nanometer-scale changes in the beads' diameters with holographic microscopy. Measured changes are interpreted with Maxwell Garnett…
The size of a probe bead reported by holographic particle characterization depends on the proportion of the surface area covered by bound target molecules and so can be used as an assay for molecular binding. We validate this technique by…
Holographic particle characterization uses quantitative analysis of holographic microscopy data to precisely and rapidly measure the diameter and refractive index of individual colloidal spheres in their native media. When this technique is…
Colloidal spheres synthesized from polymer gels swell by absorbing molecules from solution. The resulting change in size can be monitored with nanometer precision using holographic video microscopy. When the absorbate is chemically similar…
Digital holographic microscopy is a fast three-dimensional (3D) imaging tool with many applications in soft matter physics. Recent studies have shown that electromagnetic scattering solutions can be fit to digital holograms to obtain the 3D…
We perform simulations to evaluate a recent experimental technique for using in-line holographic microscopy and an effective-sphere model to measure the population-averaged fractal dimension $D_f$ of an ensemble of colloidal fractal…
In-line holographic microscopy provides an unparalleled wealth of information about the properties of colloidal dispersions. Analyzing one colloidal particle's hologram with the Lorenz-Mie theory of light scattering yields the particle's…
We build an effective medium theory for two-dimensional photonic crystals comprising a rectangular lattice of dielectric cylinders with the incident electric field polarized along the axis of the cylinders. In particular, we discuss the…
We demonstrate precise measurements of the size and refractive index of individual dimpled colloidal spheres using holographic characterization techniques developed for ideal spheres.
We report efforts to quantify the loading of cell-sized lipid vesicles using in-line digital holographic microscopy. This method does not require fluorescent reporters, fluorescent tracers, or radioactive tracers. A single-color LED light…
We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal sphere's radius and…
Label-free biosensors enable the monitoring of biomolecular interactions in real-time, which is key to the analysis of the binding characteristics of biomolecules. While refractometric optical biosensors are sensitive and well-established,…
Enhanced optical absorption of molecules in the vicinity of metallic nanostructures is key to a number of surface-enhanced spectroscopies and of great general interest to the fields of plasmonics and nano-optics. Yet, experimental access to…
Current effective medium theories for nano-shells are reviewed. A new method for calculating the effective dielectric function of a core-shell nanoparticle is presented and compared with existing theories showing clear advantages in most…
We study the extinction efficiencies as well as scattering properties of particles of different porosity. Calculations are performed for porous pseudospheres with small size (Rayleigh) inclusions using the discrete dipole approximation.…
Effective-medium theory pertains to the theoretical modelling of homogenization, which aims to replace an inhomogeneous structure of subwavelength-scale constituents with a homogeneous effective medium. The effective-medium theory is…
Interferometric scattering microscopy has been a very promising technology for highly sensitive label-free imaging of a broad spectrum of biological nanoparticles from proteins to viruses in a high-throughput manner. Although it can reveal…
Digital holographic microscopy is a powerful tool for characterizing transparent and reflective phase objects. Its ability to reconstruct amplitude and phase can also offer great insight into wavefront shaping and design of all-dielectric…
Conventional lithography methods involving pattern transfer through resist templating face challenges of material compatibility with various process solvents. Other approaches of direct material writing often compromise pattern complexity…