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Exposure to laser light alters cell culture examination via optical microscopic imaging techniques, also based on label-free coherent digital holography. To mitigate this detrimental feature, researchers tend to use a broader spectrum and…

Speckle-correlation imaging techniques are widely used for non-invasive imaging through complex scattering media. While light propagation through multimode fibers and scattering media share many analogies, reconstructing images through…

The size of a probe bead reported by holographic particle characterization depends on the proportion of the surface area covered by bound target molecules and so can be used as an assay for molecular binding. We validate this technique by…

Soft Condensed Matter · Physics 2020-07-21 Kaitlynn Snyder , Rushna Quddus , Andrew D. Hollingsworth , Kent Kirshenbaum , David G. Grier

We propose a new learning-based approach for 3D particle field imaging using holography. Our approach uses a U-net architecture incorporating residual connections, Swish activation, hologram preprocessing, and transfer learning to cope with…

Image and Video Processing · Electrical Eng. & Systems 2020-02-19 Siyao Shao , Kevin Mallery , Santosh Kumar , Jiarong Hong

Owing to its capacity for unique (bio)-chemical specificity, microscopy withmid-IR illumination holds tremendous promise for a wide range of biomedical and industrial applications. The primary limitation, however, remains detection; with…

We propose and demonstrate two methods for modal decomposition in multi-mode fibres. Linearly polarized modes propagating in a slightly multi-mode fibre are easily retrieved from intensity measurements at the fibre output surface. The first…

Polarisation holography generally demands polarisation-sensitive holograms for reconstructing either polarisation-multiplexed holographic images or polarisation-sensitive image channels. To date, polarisation holography is underpinned by…

Optics · Physics 2022-07-22 Haoran Ren

A simple imaging system together with complex semidefinite programming is used to generate the transmission matrix of a multimode fiber. Once the transmission matrix is acquired, we can modulate the phase of the input signal to induce…

In this paper, a review of indirect microwave holography for through-wall imaging is presented. Indirect microwave holography is an imaging technique, enabling the complex object scattered fields (amplitude and phase) to be mathematically…

Applied Physics · Physics 2019-08-27 Okan Yurduseven , Michael Elsdon

The use of individual multimode optical fibers in endoscopy applications has the potential to provide highly miniaturized and noninvasive probes for microscopy and optical micromanipulation. A few different strategies have been proposed…

The use of multimode fibers offers advantages in the field of communication technology in terms of transferable information density and information security. For applications using physical layer security or mode division multiplexing, the…

Image and Video Processing · Electrical Eng. & Systems 2021-04-21 Stefan Rothe , Qian Zhang , Nektarios Koukourakis , Jürgen W. Czarske

We report on local superficial blood flow monitoring in biological tissue from laser Doppler holographic imaging. In time averaging recording conditions, holography acts as a narrowband bandpass filter, which, combined with a frequency…

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

A holographic microscope captures interference patterns, or holograms, that encode three-dimensional (3D) information about the object being viewed. Computation is essential to extracting that 3D information. By wrapping low-level…

Image and Video Processing · Electrical Eng. & Systems 2018-06-04 Solomon Barkley , Thomas G. Dimiduk , Jerome Fung , David M. Kaz , Vinothan N. Manoharan , Ryan McGorty , Rebecca W. Perry , Anna Wang

This work demonstrates an application of near field indirect microwave holography for the detection of malignant tissues in the human breast in an effective way. The holograms are recorded by two directive antennas aligned along each…

Lensless digital holographic microscopy (LDHM) relies on interference between an unscattered reference wave and a weakly scattered object wave - an assumption that rapidly fails in turbid samples under multiple scattering. To overcome this…

Single-shot and incoherent imaging are two important properties for digital holography technique to extend its application range. In this paper, a simple and compact in-line Fresnel incoherent digital holographic recording system with…

Optics · Physics 2018-11-19 Dong Liang , Qiu Zhang , Jun Liu

In this work we demonstrate 3D imaging using two-photon excitation through a 20 cm long multimode optical fiber (MMF) of 350 micrometers diameter. The imaging principle is similar to single photon fluorescence through a MMF, except that a…

Optics · Physics 2016-01-20 Edgar E. Morales-Delgado , Demetri Psaltis , Christophe Moser

We have extended Laser Doppler holographic microscopy to transmission geometry. The technique is validated with living fish embryos imaged by a modified upright bio-microcope. By varying the frequency of the holographic reference beam, and…

Optics · Physics 2014-05-01 Nicolas Verrier , Daniel Alexandre , Michel Gross

Second harmonic generation (SHG) microscopy is a valuable tool for optical microscopy. SHG microscopy is normally performed as a point scanning imaging method, which lacks phase information and is limited in spatial resolution by the…

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