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We investigate the translocation of a single stranded DNA through a pore which fluctuates between two conformations, using coupled master equations. The probability density function of the first passage times (FPT) of the translocation…

Statistical Mechanics · Physics 2007-05-23 O. Flomenbom , J. Klafter

We investigate the translocation of a single stranded DNA (ssDNA) through a pore, which fluctuates between two conformations, by using coupled master equations (ME). The probability density function (PDF) of the first passage times (FPT) of…

Subcellular Processes · Quantitative Biology 2007-05-23 O. Flomenbom , J. Klafter

DNA strand displacement is a key reaction in DNA homologous recombination and DNA mismatch repair and is also heavily utilized in DNA-based computation and locomotion. Despite its ubiquity in science and engineering, sequence-dependent…

Biological Physics · Physics 2016-04-13 Bo Broadwater , Harold Kim

Motivated by experiments in which single-stranded DNA with a short hairpin loop at one end undergoes unforced diffusion through a narrow pore, we study the first passage times for a particle, executing one-dimensional brownian motion in an…

Biomolecules · Quantitative Biology 2014-11-18 Rhonald C. Lua , Alexander Y. Grosberg

Many biological processes involve one dimensional diffusion over a correlated inhomogeneous energy landscape with a correlation length $\xi_c$. Typical examples are specific protein target location on DNA, nucleosome repositioning, or DNA…

Biomolecules · Quantitative Biology 2007-05-23 Michael Slutsky , Mehran Kardar , Leonid A. Mirny

In this short note, a correction is made to the recently proposed solution [1] to a 1D biased diffusion model for linear DNA translocation and a new analysis will be given to the data in [1]. It was pointed out [2] by us recently that this…

Soft Condensed Matter · Physics 2013-08-23 Daniel Y. Ling , X. S. Ling

Long DNA molecules can be mapped by cutting them with restriction enzymes inside a narrow channel. Once cut, the individual fragments thus produced move away from each other due to diffusion and entropic effects. We investigate how long it…

Soft Condensed Matter · Physics 2025-06-11 Hanyang. Wang , Gary W Slater

Solid-state nanopores are single molecule sensors that measure changes in ionic current as charged polymers such as DNA pass through. Here, we present comprehensive experiments on the length, voltage and salt dependence of the frequency of…

Biological Physics · Physics 2016-03-23 Nicholas A. W. Bell , Murugappan Muthukumar , Ulrich F. Keyser

Motivated by recent single molecule studies of proteins sliding on a DNA molecule, we explore the targeting dynamics of N particles ("proteins") sliding diffusively along a line ("DNA") in search of their target site (specific target…

Statistical Mechanics · Physics 2009-11-11 Igor M. Sokolov , Ralf Metzler , Kiran Pant , Mark C. Williams

We perform a spatially resolved simulation study of an AND gate based on DNA strand displacement using several lengths of the toehold and the adjacent domains. DNA strands are modelled using a coarse-grained dynamic bonding model {[}C.…

Biological Physics · Physics 2013-01-01 Carsten Svaneborg , Harold Fellermann , Steen Rasmussen

We study voltage driven translocation of a single stranded (ss) DNA through a membrane channel. Our model, based on a master equation (ME) approach, investigates the probability density function (pdf) of the translocation times, and shows…

Statistical Mechanics · Physics 2007-05-23 O. Flomenbom , J. Klafter

The standard setup for single-file diffusion is diffusing particles in one dimension which cannot overtake each other, where the dynamics of a tracer (tagged) particle is of main interest. In this article we generalise this system and…

Statistical Mechanics · Physics 2015-06-18 Robin Forsling , Lloyd Sanders , Tobias Ambjörnsson , Ludvig Lizana

The first-passage time (FPT), i.e., the moment when a stochastic process reaches a given threshold value for the first time, is a fundamental mathematical concept with immediate applications. In particular, it quantifies the statistics of…

Subcellular Processes · Quantitative Biology 2018-12-05 Denis S. Grebenkov , Ralf Metzler , Gleb Oshanin

DNA and RNA are generally regarded as central molecules in molecular biology. Recent advancements in the field of DNA/RNA nanotechnology successfully used DNA/RNA as programmable molecules to construct molecular machines and nanostructures…

Biomolecules · Quantitative Biology 2019-04-01 Fan Hong , Petr Šulc

Recent single molecule experiments probing the passage process of a short single-stranded DNA (ssDNA) through a membrane channel (translocation) allow to measure the passage time distribution. Building on a recent modelling approach (D. K.…

Statistical Mechanics · Physics 2009-11-10 Ralf Metzler , Joseph Klafter

Single-molecule F\"orster Resonance Energy Transfer (smFRET) is ideally suited to resolve the dynamics of biomolecules. A significant challenge to date is capturing and quantifying the exchange between multiple conformational states, mainly…

A DNA polymerase (DNAP) replicates a template DNA strand. It also exploits the template as the track for its own motor-like mechanical movement. In the polymerase mode it elongates the nascent DNA by one nucleotide in each step. But,…

Subcellular Processes · Quantitative Biology 2013-10-16 Ajeet K. Sharma , Debashish Chowdhury

DNA capture with high fidelity is an essential part of nanopore translocation. We report several important aspects of the capture process and subsequent translocation of a model DNA polymer through a solid-state nanopore in presence of an…

Soft Condensed Matter · Physics 2022-07-06 Swarnadeep Seth , Aniket Bhattacharya

A detailed description of the conformational plasticity of double stranded DNA (ds) is a necessary framework for understanding protein-DNA interactions. Until now, however structure and kinetics of the transition from the basic conformation…

Soft Condensed Matter · Physics 2011-03-04 Pasquale Bianco , Lorenzo Bongini , Luca Melli , Mario Dolfi , Vincenzo Lombardi
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