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Mitochondria are complex organelles, and their proteomics analysis requires a combination of techniques. The emphasis in this chapter is made first on mitochondria preparation from cultured mammalian cells, then on the separation of the…

Genomics · Quantitative Biology 2008-07-09 Thierry Rabilloud

We investigate the final stage of cytokinesis in two types of amoeba, pointing out the existence of biphasic furrow contraction. The first phase is characterized by a constant contraction rate, is better studied, and seems universal to a…

Cell Behavior · Quantitative Biology 2007-05-23 D. Biron , P. Libros , D. Sagi , D. Mirelman , E. Moses

Bacterial biofilms collectively develop distinct and ordered structures, including fibers, bundles, and branches. Often, it is unclear how these structural motifs convey specific advantages to bacterial strains under challenging conditions.…

Soft Condensed Matter · Physics 2022-11-16 Mustafa Basaran , Tevfik Can Yüce , Yusuf Ilker Yaman , Roman Vetter , Askin Kocabas

Growth in bacterial populations generally depends on the environment (availability and quality of nutrients, presence of a toxic inhibitor, product inhibition..). Here, we build a model to describe the action of a bacteriostatic antibiotic,…

Biological Physics · Physics 2025-06-25 Barnabe Ledoux , David Lacoste

Phagocytosis is the fundamental cellular process by which eukaryotic cells bind and engulf particles by their cell membrane. Particle engulfment involves particle recognition by cell-surface receptors, signaling and remodeling of the actin…

Cell Behavior · Quantitative Biology 2010-11-02 Sylvain Tollis , Anna E. Dart , George Tzircotis , Robert G. Endres

Understanding design principles of complex cellular organization is one of the major challenges in biology. Recent analysis of the large-scale cellular organization has revealed the scale-free nature and robustness of metabolic and protein…

Molecular Networks · Quantitative Biology 2007-05-23 Hiroki R. Ueda , John B. Hogenesch

Biological membranes are complex, dynamic structures essential for cellular compartmentalization, signaling, and mechanical integrity. The molecular organization of eukaryotic membranes has been extensively studied, including the lipid…

Soft Condensed Matter · Physics 2025-08-05 Nicolo Tormena , Teuta Pilizota , Kislon Voïtchovsky

Bacterial growth environment strongly influences the efficacy of antibiotic treatment, with slow growth often being associated with decreased susceptibility. Yet in many cases the connection between antibiotic susceptibility and pathogen…

Cell Behavior · Quantitative Biology 2015-05-06 Philip Greulich , Matthew Scott , Martin R. Evans , Rosalind J. Allen

In this article we highlight chemotaxis (cellular movement) as a rich source of potential engineering applications and computational models, highlighting current research and possible future work. We first give a brief description of the…

Cell Behavior · Quantitative Biology 2007-05-23 Martyn Amos , David A. Hodgson , Alan Gibbons

Several bacteria and bacteria strands form biofilms in different environmental conditions, e.g. pH, temperature, nutrients, etc. Biofilm growth, therefore, is an extremely robust process. Because of this, while biofilm growth is a complex…

From biofilm and colony formation in bacteria to wound healing and embryonic development in multicellular organisms, groups of living cells must often move collectively. While considerable study has probed the biophysical mechanisms of how…

Biological Physics · Physics 2022-06-08 Benedikt Sabass , Howard A. Stone , Joshua W. Shaevitz

Biochemical processes typically involve huge numbers of individual reversible steps, each with its own dynamical rate constants. For example, kinetic proofreading processes rely upon numerous sequential reactions in order to guarantee the…

Molecular Networks · Quantitative Biology 2009-12-28 Golan Bel , Brian Munsky , Ilya Nemenman

Many organic molecules have been observed in the interstellar medium thanks to advances in radioastronomy, and very recently the presence of urea was also suggested. While those molecules were observed, it is not clear what the mechanisms…

Astrophysics of Galaxies · Physics 2018-02-21 Flavio Siro Brigiano , Yannick Jeanvoine , Antonio Largo , Riccardo Spezia

Motivated by observations of the dynamics of {\it Myxococcus xanthus}, we present a self-interacting random walk model that describes the competition between chemokinesis and chemotaxis. Cells are constrained to move in one dimension, but…

Statistical Mechanics · Physics 2016-08-31 Maria R. D'Orsogna , Marc Suchard , Tom Chou

Stable polyyne chains terminated with biphenyl end groups (a,u-biphenylpolyynes) were synthesized in a single step through a simple procedure by using the Cadiot-Chodkiewicz reaction conditions. The a,ubiphenylpolyynes were separated…

Materials Science · Physics 2018-03-20 Franco Cataldo , Ornella Ursini , Alberto Milani , Carlo S. Casari

Bacterial conglomerates such as biofilms and microcolonies are ubiquitous in nature and play an important role in industry and medicine. In contrast to well-mixed, diluted cultures routinely used in microbial research, bacteria in a…

Biological Physics · Physics 2017-01-13 Fred F. Farrell , Matti Gralka , Oskar Hallatschek , Bartlomiej Waclaw

Biophysicists and structural biologists increasingly acknowledge the role played by the mechanical properties of macromolecules as a critical element in many biological processes. This change has been brought about, in part, by the advent…

Biomolecules · Quantitative Biology 2009-11-10 Prashant K. Purohit , Jane' Kondev , Rob Phillips

Many biological electron transfer (ET) reactions are mediated by metal centres in proteins. NADH:ubiquinone oxidoreductase (complex I) contains an intramolecular chain of seven iron-sulphur (FeS) clusters, one of the longest chains of metal…

Biomolecules · Quantitative Biology 2011-11-09 Ross Dorner , John Goold , Libby Heaney , Tristan Farrow , Philippa G. Roberts , Judy Hirst , Vlatko Vedral

Folding of Ubiquitin (Ub) is investigated at low and neutral pH at different temperatures using simulations of the coarse-grained Self-Organized-Polymer model with side chains. The calculated radius of gyration, showing dramatic variations…

Biomolecules · Quantitative Biology 2015-07-09 Govardhan Reddy , D. Thirumalai

While modern structural biology has provided us with a rich and diverse picture of membrane proteins, the biological function of membrane proteins is often influenced by the mechanical properties of the surrounding lipid bilayer. Here we…

Biomolecules · Quantitative Biology 2013-05-27 Christoph A. Haselwandter , Rob Phillips