Related papers: Multiscale Modeling and Analysis for High-fidelity…
Interferometric scattering (iSCAT) microscopy is an emerging label-free technique optimized for the sensitive detection of nano-matter. Previous iSCAT studies have approximated the point spread function in iSCAT by a Gaussian intensity…
Interferometric scattering (iSCAT) microscopy is a powerful tool for label-free sensitive detection and imaging of nanoparticles to high spatio-temporal resolution. As it was born out of detection principles central to conventional…
Interferometric scattering microscopy (iSCAT) can image the dynamics of nanometer-scale systems. The typical approach to analyzing interferometric images involves intensive processing, which discards data and limits the precision of…
Tracking nanoparticle movement is highly desirable in many scientific areas, and various imaging methods have been employed to achieve this goal. Interferometric scattering (iSCAT) microscopy has been particularly successful in combining…
Coherent interferometric scattering microscopy (iscat) enables nanoparticle tracking on microsecond timescales and with nanometer precision, and has become a key tool in structural and cellular biophysics. The achievable localization…
Interferometric scattering (iSCAT) microscopy has undergone significant development in recent years. It is a promising technique for imaging and tracking nanoscopic label-free objects with nanometer localization precision. The current…
We provide detailed experimental guidelines for implementing digital holography in the context of high-sensitivity interferometric scattering (iSCAT) based nanosizing applications. Our approach relies on interferometry via the highly…
Interferometric scattering (iSCAT) microscopy is currently among the most powerful techniques available for achieving high-sensitivity single-particle localization. This capability is realized through homodyne detection, where interference…
Direct optical detection and imaging of single nanoparticles on substrate in wide field underpin vast applications across different research fields. However, the speckles originating from the unavoidable random surface undulations of the…
Interferometric scattering microscopy is a powerful technique that enables various applications, such as mass photometry and particle tracking. Here we present a numerical toolbox to simulate images obtained in interferometric scattering,…
Interferometric scattering microscopy was widely applied in nanoscopic detection and tracking due to its high sensitivity and label-free manner. The sensitivity is limited by contrast. Oblique illumination provided high contrast with lower…
Reflection phase imaging provides label-free, high-resolution characterization of biological samples, typically using interferometric-based techniques. Here, we investigate reflection phase microscopy from intensity-only measurements under…
Quantitative phase microscopy (QPM) and interferometric scattering (iSCAT) microscopy are powerful label-free imaging techniques and are widely used for biomedical applications. Each method, however, possesses distinct limitations: QPM,…
In this study we present an interferometric technique based on multiple wavelengths to capture the transient free surface contour of nanoliter drops spreading on a wettable surface, in particular close to the three-phase contact line.…
Label-free imaging of rapidly moving, sub-diffraction sized structures has important applications in both biology and material science, as it removes the limitations associated with fluorescence tagging. However, unlabeled nanoscale…
High-fidelity 3D Gaussian Splatting methods excel at capturing fine textures but often overlook model compactness, resulting in massive splat counts, bloated memory, long training, and complex post-processing. We present Micro-Splatting:…
Imaging of the optical properties of individual nanosystems beyond fluorescence can provide a wealth of information. However, the minute signals for absorption and dispersion are challenging to observe, and only specialized techniques…
The ability to observe rotational dynamics and measure underlying torques is a crucial component in understanding the function and mechanics of nanoscale systems. Yet, direct observation of rotational dynamics at the single-molecule level…
Specialized applications of nanoparticles often call for particular, well-characterized particle size distributions in solution. But, this property can prove difficult to measure. High-throughput methods, such as dynamic light scattering,…
Energy carrier evolution is crucial for material performance. Ultrafast microscopy has been widely applied to visualize the spatiotemporal evolution of energy carriers. However, direct imaging of small amounts of energy carriers on…