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Localization microscopy is an imaging technique in which the positions of individual nanoscale point emitters (e.g. fluorescent molecules) are determined at high precision from their images. This is the key ingredient in…

Image and Video Processing · Electrical Eng. & Systems 2020-10-01 Elias Nehme , Daniel Freedman , Racheli Gordon , Boris Ferdman , Lucien E. Weiss , Onit Alalouf , Reut Orange , Tomer Michaeli , Yoav Shechtman

Imaging in thick biological tissues is often degraded by sample-induced aberrations, which reduce image quality and resolution, particularly in super-resolution techniques. While hardware-based adaptive optics, which correct aberrations…

Ultrasound Localization Microscopy (ULM) enables imaging of vascular structures in the micrometer range by accumulating contrast agent particle locations over time. Precise and efficient target localization accuracy remains an active…

Image and Video Processing · Electrical Eng. & Systems 2023-11-08 Christopher Hahne , Georges Chabouh , Olivier Couture , Raphael Sznitman

In single-molecule super-resolution microscopy, engineered point-spread functions (PSFs) are designed to efficiently encode new molecular properties, such as 3D orientation, into complex spatial features captured by a camera. To fully…

Image and Video Processing · Electrical Eng. & Systems 2019-07-16 Hesam Mazidi , Eshan S. King , Oumeng Zhang , Arye Nehorai , Matthew D. Lew

Linear spectral unmixing is an essential technique in hyperspectral image processing and interpretation. In recent years, deep learning-based approaches have shown great promise in hyperspectral unmixing, in particular, unsupervised…

Image and Video Processing · Electrical Eng. & Systems 2022-08-10 Lin Qi , Feng Gao , Junyu Dong , Xinbo Gao , Qian Du

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester

We report a new coherent imaging technique, termed ptychographic structured modulation (PSM), for quantitative super-resolution microscopy. In this technique, we place a thin diffuser (i.e., a scattering lens) in between the sample and the…

Image and Video Processing · Electrical Eng. & Systems 2019-07-26 Pengming Song , Shaowei Jiang , He Zhang , Zichao Bian , Chengfei Guo , Kazunori Hoshino , Guoan Zheng

Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…

Optics · Physics 2024-05-01 Tengfei Wu , YoonSeok Baek , Fei Xia , Sylvain Gigan , Hilton B. de Aguiar

Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…

Computer Vision and Pattern Recognition · Computer Science 2025-12-19 Paloma Casteleiro Costa , Parnian Ghapandar Kashani , Xuhui Liu , Alexander Chen , Ary Portes , Julien Bec , Laura Marcu , Aydogan Ozcan

Imaging through scattering is an important, yet challenging problem. Tremendous progress has been made by exploiting the deterministic input-output "transmission matrix" for a fixed medium. However, this "one-to-one" mapping is highly…

Image and Video Processing · Electrical Eng. & Systems 2018-09-27 Yunzhe Li , Yujia Xue , Lei Tian

Recent learning-based lossless image compression methods encode an image in the unit of subimages and achieve comparable performances to conventional non-learning algorithms. However, these methods do not consider the performance drop in…

Image and Video Processing · Electrical Eng. & Systems 2021-12-14 Hochang Rhee , Yeong Il Jang , Seyun Kim , Nam Ik Cho

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

Lensless imaging is a popular research field for the advantages of small size, wide field-of-view and low aberration in recent years. However, some traditional lensless imaging methods suffer from slow convergence, mechanical errors and…

Skin lesion segmentation (SLS) in dermoscopic images is a crucial task for automated diagnosis of melanoma. In this paper, we present a robust deep learning SLS model, so-called SLSDeep, which is represented as an encoder-decoder network.…

Fourier ptychographic microscopy allows for the collection of images with a high space-bandwidth product at the cost of temporal resolution. In Fourier ptychographic microscopy, the light source of a conventional widefield microscope is…

Image and Video Processing · Electrical Eng. & Systems 2019-01-30 Yi Fei Cheng , Megan Strachan , Zachary Weiss , Moniher Deb , Dawn Carone , Vidya Ganapati

Benefited from the deep learning, image Super-Resolution has been one of the most developing research fields in computer vision. Depending upon whether using a discriminator or not, a deep convolutional neural network can provide an image…

Computer Vision and Pattern Recognition · Computer Science 2020-04-28 Zhi-Song Liu , Wan-Chi Siu , Li-Wen Wang , Chu-Tak Li , Marie-Paule Cani , Yui-Lam Chan

We exploit memory effect speckle correlations for the imaging of incoherent linear (single-photon) fluorescent sources behind scattering tissue. While memory effect-based imaging techniques have been heavily studied in the past, for thick…

Live-cell imaging of multiple subcellular structures is essential for understanding subcellular dynamics. However, the conventional multi-color sequential fluorescence microscopy suffers from significant imaging delays and limited number of…

Subcellular Processes · Quantitative Biology 2025-01-13 Mingyang Chen , Luhong Jin , Xuwei Xuan , Defu Yang , Yun Cheng , Ju Zhang

Image fusion aims at estimating a high-resolution spectral image from a low-spatial-resolution hyperspectral image and a low-spectral-resolution multispectral image. In this regard, compressive spectral imaging (CSI) has emerged as an…

Image and Video Processing · Electrical Eng. & Systems 2021-08-04 Juan Marcos Ramírez , José Ignacio Martínez Torre , Henry Arguello Fuentes

With super-resolution optical microscopy, it is now possible to observe molecular interactions in living cells. The obtained images have a very high spatial precision but their overall quality can vary a lot depending on the structure of…

Computer Vision and Pattern Recognition · Computer Science 2018-03-30 Louis-Émile Robitaille , Audrey Durand , Marc-André Gardner , Christian Gagné , Paul De Koninck , Flavie Lavoie-Cardinal