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Relying on either deep models or physical models are two mainstream approaches for solving inverse sample reconstruction problems in programmable illumination computational microscopy. Solutions based on physical models possess strong…

Image and Video Processing · Electrical Eng. & Systems 2024-03-21 Ruiqing Sun , Delong Yang , Shaohui Zhang , Qun Hao

Fourier Ptychographic Microscopy (FPM) is a computational imaging method that is able to super-resolve features beyond the diffraction-limit set by the objective lens of a traditional microscope. This is accomplished by using synthetic…

Signal Processing · Electrical Eng. & Systems 2019-04-09 Michael Kellman , Emrah Bostan , Michael Chen , Laura Waller

Deep-learning algorithms enable precise image recognition based on high-dimensional hierarchical image features. Here, we report the development and implementation of a deep-learning-based image segmentation algorithm in an autonomous…

Image and Video Processing · Electrical Eng. & Systems 2020-03-26 Satoru Masubuchi , Eisuke Watanabe , Yuta Seo , Shota Okazaki , Takao Sasagawa , Kenji Watanabe , Takashi Taniguchi , Tomoki Machida

We discuss recently emerging applications of the state-of-art deep learning methods on optical microscopy and microscopic image reconstruction, which enable new transformations among different modes and modalities of microscopic imaging,…

Machine Learning · Computer Science 2018-07-23 Yair Rivenson , Aydogan Ozcan

Fourier ptychographic microscopy (FPM) is a computational approach geared towards creating high-resolution and large field-of-view images without mechanical scanning. To acquire color images of histology slides, it often requires sequential…

Image and Video Processing · Electrical Eng. & Systems 2020-10-28 Ruihai Wang , Pengming Song , Shaowei Jiang , Chenggang Yan , Jiakai Zhu , Chengfei Guo , Zichao Bian , Tianbo Wang , Guoan Zheng

Fluorescence microscopy plays a vital role in understanding the subcellular structures of living cells. However, it requires considerable effort in sample preparation related to chemical fixation, staining, cost, and time. To reduce those…

Image and Video Processing · Electrical Eng. & Systems 2020-12-02 Thanh Nguyen , Vy Bui , Anh Thai , Van Lam , Christopher B. Raub , Lin-Ching Chang , George Nehmetallah

Deep learning algorithms offer a powerful means to automatically analyze the content of medical images. However, many biological samples of interest are primarily transparent to visible light and contain features that are difficult to…

Computer Vision and Pattern Recognition · Computer Science 2017-09-22 Roarke Horstmeyer , Richard Y. Chen , Barbara Kappes , Benjamin Judkewitz

At the heart of the success of deep learning is the quality of the data. Through data augmentation, one can train models with better generalization capabilities and thus achieve greater results in their field of interest. In this work, we…

Computer Vision and Pattern Recognition · Computer Science 2021-10-28 George Chogovadze , Rémi Pautrat , Marc Pollefeys

Microscopy enables direct observation of cellular morphology in 3D, with transmitted-light methods offering low-cost, minimally invasive imaging and fluorescence microscopy providing specificity and contrast. Virtual staining combines these…

Fluorescence labeling is the standard approach to reveal cellular structures and other subcellular constituents for microscopy images. However, this invasive procedure may perturb or even kill the cells and the procedure itself is highly…

Image and Video Processing · Electrical Eng. & Systems 2024-06-25 Han Liu , Hao Li , Jiacheng Wang , Yubo Fan , Zhoubing Xu , Ipek Oguz

In recent years, significant progress has been made in image recognition technology based on deep neural networks. However, improving recognition performance under low-light conditions remains a significant challenge. This study addresses…

Computer Vision and Pattern Recognition · Computer Science 2025-01-09 Seitaro Ono , Yuka Ogino , Takahiro Toizumi , Atsushi Ito , Masato Tsukada

Visualizing the details of different cellular structures is of great importance to elucidate cellular functions. However, it is challenging to obtain high quality images of different structures directly due to complex cellular environments.…

Image and Video Processing · Electrical Eng. & Systems 2019-10-01 Yi Liu , Hao Yuan , Zhengyang Wang , Shuiwang Ji

Fluorescent Neuronal Cells v2 is a collection of fluorescence microscopy images and the corresponding ground-truth annotations, designed to foster innovative research in the domains of Life Sciences and Deep Learning. This dataset…

In this work, we propose a step towards a more accurate prediction of the environment light given a single picture of a known object. To achieve this, we developed a deep learning method that is able to encode the latent space of indoor…

Computer Vision and Pattern Recognition · Computer Science 2018-08-14 Henrique Weber , Donald Prévost , Jean-François Lalonde

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, and complexity, and thus cannot fulfill the emerging need of…

Optics · Physics 2022-09-09 Yujia Xue , Qianwan Yang , Guorong Hu , Kehan Guo , Lei Tian

Photonic brain-inspired platforms are emerging as novel analog computing devices, enabling fast and energy-efficient operations for machine learning. These artificial neural networks generally require tailored optical elements, such as…

Emerging Technologies · Computer Science 2021-07-30 Davide Pierangeli , Giulia Marcucci , Claudio Conti

Reliable analysis of intracellular dynamic processes in time-lapse fluorescence microscopy images requires complete and accurate tracking of all small particles in all time frames of the image sequences. A fundamental first step towards…

Image and Video Processing · Electrical Eng. & Systems 2024-08-16 Yao Yao , Ihor Smal , Ilya Grigoriev , Anna Akhmanova , Erik Meijering

Photonic device development (PDD) has achieved remarkable success in designing and implementing new devices for controlling light across various wavelengths, scales, and applications, including telecommunications, imaging, sensing, and…

Fluorescence microscopy allows for a detailed inspection of cells, cellular networks, and anatomical landmarks by staining with a variety of carefully-selected markers visualized as color channels. Quantitative characterization of…

Computer Vision and Pattern Recognition · Computer Science 2021-08-26 Alvaro Gomariz , Tiziano Portenier , Patrick M. Helbling , Stephan Isringhausen , Ute Suessbier , César Nombela-Arrieta , Orcun Goksel

Electron and scanning probe microscopy produce vast amounts of data in the form of images or hyperspectral data, such as EELS or 4D STEM, that contain information on a wide range of structural, physical, and chemical properties of…

Machine Learning · Computer Science 2023-06-09 Arpan Biswas , Maxim Ziatdinov , Sergei V. Kalinin