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Digital holographic microscopy is an emerging potentially low-cost alternative to conventional light microscopy for micro-object imaging on earth, underwater and in space. Immediate access to micron-scale objects however requires a…

Optics · Physics 2019-04-10 Stephan Amann , Max von Witzleben , and Stefan Breuer

Optical phase-space functions describe spatial and angular information simultaneously; examples include light fields in ray optics and Wigner functions in wave optics. Measurement of phase-space enables digital refocusing, aberration…

Optics · Physics 2017-08-02 Hsiou-Yuan Liu , Jingshan Zhong , Laura Waller

The trade-off between throughput and image quality is an inherent challenge in microscopy. To improve throughput, compressive imaging under-samples image signals; the images are then computationally reconstructed by solving a regularized…

Image and Video Processing · Electrical Eng. & Systems 2023-03-07 Udith Haputhanthri , Andrew Seeber , Dushan Wadduwage

Flexible fiber-optic endoscopes provide a minimally-invasive solution for imaging at depths beyond the reach of conventional microscopes. Current endoscopes require focusing and/or scanning mechanisms at the distal end, which limit…

Optical imaging through complex scattering media is one of the major technical challenges with important applications in many research fields, ranging from biomedical imaging, astronomical telescopy, and spatially multiplex optical…

Photoacoustic microscopy with large depth of focus is significant to the biomedical research. The conventional optical-resolution photoacoustic microscope (OR-PAM) suffers from limited depth of field (DoF) since the employed focused…

Image and Video Processing · Electrical Eng. & Systems 2020-09-07 Xianlin Song , Jianshuang Wei , Lingfang Song

Lensless imaging enables exceptionally compact fluorescence sensors, advancing applications in \textit{in vivo} imaging and low-cost, point-of-care diagnostics. These sensors require a filter to block the excitation light while passing…

Image and Video Processing · Electrical Eng. & Systems 2026-02-20 Lukas Harris , Micah Roschelle , Jack Bartley , Mekhail Anwar

Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain…

Physics Education · Physics 2019-07-24 Stephane Perrin , Keshia Badu , Paul Montgomery , Sylvain Lecler

Existing three-dimensional (3-D) compressive sensing-based millimeter-wave (MMW) imaging methods require a large-scale storage of the sensing matrix and immense computations owing to the high dimension matrix-vector model employed in the…

Signal Processing · Electrical Eng. & Systems 2018-12-26 Shiyong Li , Guoqiang Zhao , Houjun Sun , Moeness Amin

Light-field microscopy (LFM) enables rapid volumetric imaging through single-frame acquisition and fast 3D reconstruction algorithms. The high speed and low phototoxicity of LFM make it highly suitable for real-time 3D fluorescence imaging,…

Optics · Physics 2025-02-24 Bohan Qu , Zhouyu Jin , You Zhou , Bo Xiong , Xun Cao

Metasurfaces offer compact flat lenses (metalenses) for miniaturized imaging systems; however, the utmost miniaturization requires not only metalenses but also a substantial reduction of free space. A Spaceplate is a flat-optics element…

Optics · Physics 2026-05-12 Imon Kalyan , Raghvendra P. Chaudhary , Nir Shitrit

Fast, volumetric imaging over large scales has been a long-standing goal in biological microscopy. Scanning techniques such as fluorescence confocal microscopy can acquire 2D images at high resolution and high speed, but extending the…

Fiber-based endoscopes utilizing multi-core fiber (MCF) bundles offer the capability to image deep within the human body, making them well-suited for imaging applications in minimally invasive surgery or diagnosis. However, the optical…

Fourier ptychographic microscopy (FPM) is a powerful computational imaging modality that achieves high space-bandwidth product imaging for biomedical samples. However, its adoption is limited by slow data acquisition due to the need for…

Optics · Physics 2025-09-09 Shi Zhao , Haowen Zhou , Changhuei Yang

Confocal and multiphoton microscopy are effective techniques to obtain high-contrast images of 2-D sections within bulk tissue. However, scattering limits their application to depths only up to ~1 millimeter. Multimode fibers make excellent…

Optics · Physics 2022-02-23 Sakshi Singh , Simon Labouesse , Rafael Piestun

Confocal microscopy is the backbone of cellular research labs across the world but unfortunately, the imaging is restricted to a single plane. Chromatic confocal microscopy offers the possibility to image multiple planes simultaneously thus…

Optics · Physics 2022-02-01 Gargi Sharma , Kanwarpal Singh

Ultrafast 3D imaging is indispensable for visualizing complex and dynamic biological processes. Conventional scanning-based techniques necessitate an inherent trade-off between acquisition speed and space-bandwidth product (SBP). Emerging…

Microscopy enables direct observation of cellular morphology in 3D, with transmitted-light methods offering low-cost, minimally invasive imaging and fluorescence microscopy providing specificity and contrast. Virtual staining combines these…

Multi-photon microscopy is a powerful technique for deep-tissue imaging, providing high spatial resolution at increased penetration depth. Nevertheless, imaging remains largely restricted to superficial tissue layers well below 1 mm.…

From biology and astronomy to quantum optics, there is a critical need for high frame rate, high quantum efficiency imaging. In practice, most cameras only satisfy one of these requirements. Here we introduce interlaced fast kinetics…

Atomic Physics · Physics 2025-05-21 Bowen Li , Lukas Palm , Marius Jürgensen , Yiming Cady Feng , Markus Greiner , Jon Simon