Related papers: Adaptive optics for multifocal plane microscopy
The imaging performance of an optical microscope can be degraded by sample-induced aberrations. A general strategy to undo the effect of these aberrations is to apply wavefront correction with a deformable mirror (DM). In most cases, the DM…
Adaptive optics (AO) is a powerful image correction technique with proven benefits for many life-science microscopy methods. However, the complexity of adding a reflective wavefront modulator and a wavefront sensor into already complicated…
We describe an adaptive optics technique for two-photon microscopy in which the deformable mirror used for aberration compensation is positioned in a plane conjugate to the plane of the aberration. We demonstrate in a proof-of-principle…
In order to increase the corrected field of view of an adaptive optics (AO) system, several deformable mirrors (DM) have to be placed in the conjugate planes of the dominant turbulent layers (multi-conjugate adaptive optics, MCAO [Beckers…
Images obtained by microscopes are generally degraded by aberrations. Adaptive optics (AO) has been widely used in to compensate for aberrations and improve reduced image quality. Requiring no separate wavefront sensor, sensorless AO…
Multiconjugate adaptive optics (MCAO) systems have the potential to deliver diffraction-limited images over much larger fields of view than traditional single conjugate adaptive optics systems. In MCAO, the high altitude deformable mirrors…
The resolution and contrast of microscope imaging is often affected by aberrations introduced by imperfect optical systems and inhomogeneous refractive structures in specimens. Adaptive optics (AO) compensates these aberrations and restores…
Light microscopy enables multifunctional imaging of biological specimens at unprecedented depths and resolutions. However, the performance of all optical methods degrades with the imaging depth due to sample-induced aberrations. Methods of…
Since the year 2000, adaptive optics (AO) has seen the emergence of a variety of new concepts addressing particular science needs; multiconjugate adaptive optics (MCAO) is one of them. By correcting the atmospheric turbulence in 3D using…
Due to turbulence in the atmosphere images taken from ground-based telescopes become distorted. With adaptive optics (AO) images can be given greater clarity allowing for better observations with existing telescopes and are essential for…
Multi-object adaptive optics (MOAO) has been demonstrated by the CANARY instrument on the William Herschel Telescope. However, for proposed MOAO systems on the next generation Extremely Large Telescopes, such as EAGLE, many challenges…
Inhomogeneities in the refractive index of a biological sample can introduce phase aberrationsin microscopy systems, severely impairing the quality of images. Adaptive optics can be employed to correct for phase aberrations and improve…
Three-photon (3-P) fluorescence microscopy enables deep in vivo imaging with subcellular resolution, but its performance is fundamentally constrained by the maximum permissible laser power required to avoid tissue heating and photodamage.…
Adaptive optics (AO) offers an opportunity to stabilize an image and maximize the spatial resolution achievable by ground based telescopes by removing the distortions due to the atmosphere. Typically, the deformable mirror in an AO system…
Astronomical telescopes suffer from a tradeoff between field of view (FoV) and image resolution: increasing the FoV leads to an optical field that is under-sampled by the science camera. This work presents a novel computational imaging…
Future large space telescopes will be equipped with adaptive optics (AO) to overcome wavefront aberrations and achieve high contrast for imaging faint astronomical objects, such as earth-like exoplanets and debris disks. In contrast to AO…
Multi-Conjugate Adaptive Optics (MCAO) is essential for increasing the corrected Field-of-View (FoV) in astronomical imaging and potentially for free-space optical communications, particularly for small-aperture, transportable systems. We…
We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive…
One important frontier for astronomical adaptive optics (AO) involves methods such as Multi-Object AO and Multi-Conjugate AO that have the potential to give a significantly larger field of view than conventional AO techniques. A second key…
Modern extreme adaptive optics (AO) systems achieving diffraction-limited performance open up new possibilities for instrumentation. Especially important for the fields of spectroscopy and interferometry is that it enables the prospect to…