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Holograms of colloidal particles can be analyzed with the Lorenz-Mie theory of light scattering to measure individual particles' three-dimensional positions with nanometer precision while simultaneously estimating their sizes and refractive…

Soft Condensed Matter · Physics 2018-07-04 Mark D. Hannel , Aidan Abdulali , Michael O'Brien , David G. Grier

We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal sphere's radius and…

Despite its potential for label-free particle diagnostics, holographic microscopy is limited by specialized processing methods that struggle to generalize across diverse settings. We introduce a deep learning architecture leveraging human…

Optics · Physics 2024-11-26 Shyam Kumar , Jiarong Hong

We propose a new learning-based approach for 3D particle field imaging using holography. Our approach uses a U-net architecture incorporating residual connections, Swish activation, hologram preprocessing, and transfer learning to cope with…

Image and Video Processing · Electrical Eng. & Systems 2020-02-19 Siyao Shao , Kevin Mallery , Santosh Kumar , Jiarong Hong

Digital holographic microscopy is a fast three-dimensional (3D) imaging tool with many applications in soft matter physics. Recent studies have shown that electromagnetic scattering solutions can be fit to digital holograms to obtain the 3D…

Colloidoscope is a deep learning pipeline employing a 3D residual Unet architecture, designed to enhance the tracking of dense colloidal suspensions through confocal microscopy. This methodology uses a simulated training dataset that…

The development of robust, real-time optical methods for the detection and tracking of particles in complex multiple scattering media is a problem of practical importance in a number of fields, including environmental monitoring, air…

Optics · Physics 2025-02-04 Mohammadrasoul Taghavi , Edwin A. Marengo

We introduce a novel framework that incorporates multiple scattering for large-scale 3D particle-localization using single-shot in-line holography. Traditional holographic techniques rely on single-scattering models which become inaccurate…

Signal Processing · Electrical Eng. & Systems 2019-06-19 Waleed Tahir , Ulugbek S. Kamilov , Lei Tian

In-line digital holography is a valuable tool for sizing, locating and tracking micro- or nano-objects in a volume. When a parametric imaging model is available, Inverse Problems approaches provide a straightforward estimate of the object…

Instrumentation and Detectors · Physics 2023-07-19 Nicolas Verrier , Corinne Fournier , Thierry Fournel

HOLODEC, an airborne cloud particle imager, captures holographic images of a fixed volume of cloud to characterize the types and sizes of cloud particles, such as water droplets and ice crystals. Cloud particle properties include position,…

Image and Video Processing · Electrical Eng. & Systems 2022-03-21 John S. Schreck , Gabrielle Gantos , Matthew Hayman , Aaron Bansemer , David John Gagne

Advanced three-dimensional (3D) tracking methods are essential for studying particle dynamics across a wide range of complex systems, including multiphase flows, environmental and atmospheric sciences, colloidal science, biological and…

Fluid Dynamics · Physics 2024-12-25 Shyam Kumar M , Jiarong Hong

Quantitative tracking of features from video images is a basic technique employed in many areas of science. Here, we present a method for the tracking of features that partially overlap, in order to be able to track so-called colloidal…

Soft Condensed Matter · Physics 2016-11-24 Casper van der Wel , Daniela J. Kraft

Confocal microscopy of colloids combined with digital image processing has become a powerful tool in soft matter physics and materials science. Together, these techniques enable locating and tracking of more than half a million individual…

Soft Condensed Matter · Physics 2016-05-10 Katharine E. Jensen , Nobutomo Nakamura

Particle size measurement based on digital holography with conventional algorithms are usually time-consuming and susceptible to noises associated with hologram quality and particle complexity, limiting its usage in a broad range of…

Applied Physics · Physics 2020-01-01 Siyao Shao , Kevin Mallery , Jiarong Hong

A holographic microscope captures interference patterns, or holograms, that encode three-dimensional (3D) information about the object being viewed. Computation is essential to extracting that 3D information. By wrapping low-level…

Image and Video Processing · Electrical Eng. & Systems 2018-06-04 Solomon Barkley , Thomas G. Dimiduk , Jerome Fung , David M. Kaz , Vinothan N. Manoharan , Ryan McGorty , Rebecca W. Perry , Anna Wang

An in-line hologram of a colloidal sphere can be analyzed with the Lorenz-Mie theory of light scattering to measure the sphere's three-dimensional position with nanometer-scale precision while also measuring its diameter and refractive…

Soft Condensed Matter · Physics 2020-12-29 Lauren E. Altman , Rushna Quddus , Fook Chiong Cheong , David G. Grier

Holographic microscopy has developed into a powerful tool for 3D particle tracking, yielding nanometer-scale precision at high frame rates. However, current particle tracking algorithms ignore the effect of the microscope objective on the…

Since holography yields exact results, even in situations where perturbation theory is not applicable, it is an ideal framework for modeling strongly correlated systems. We extend previous holographic methods to take the dynamical charge…

High Energy Physics - Theory · Physics 2018-12-26 Ulf Gran , Marcus Tornsö , Tobias Zingg

Holographic particle characterization uses quantitative analysis of holographic microscopy data to precisely and rapidly measure the diameter and refractive index of individual colloidal spheres in their native media. When this technique is…

Soft Condensed Matter · Physics 2022-10-19 Rafe Abdulali , Lauren E. Altman , David G. Grier

Colloidal lithography [1] is how patterns are reproduced in a variety of natural systems and is used more and more as an efficient fabrication tool in bio-, opto-, and nano-technology. Nanoparticles in the colloid are made to form a mask on…

Mesoscale and Nanoscale Physics · Physics 2013-11-27 A. Iovan , M. Fischer , R. Lo Conte , V. Korenivski
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