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In order to relate nanoparticle properties to function, fast and detailed particle characterization, is needed. The ability to characterize nanoparticle samples using optical microscopy techniques has drastically improved over the past few…

Optical coherence tomography (OCT) is a prevalent, interferometric, high-resolution imaging method with broad biomedical applications. Nonetheless, OCT images suffer from an artifact, called speckle which degrades the image quality. Digital…

Phase sensitive detection in spectral domain optical coherence tomography (SD-OCT) is a powerful method for functional imaging of biological events with high spatiotemporal resolution. The depth-dependent signal-to-noise ratio (SNR) is a…

In the past years, optical fluorescence microscopy (OFM) made steady progress towards increasing the localisation precision of fluorescent emitters in biological samples. The high precision achieved by these techniques has prompted new…

We propose and demonstrate a novel technique that combines Raman scattering and optical cycling in molecules with diagonal Franck-Condon factors. This resonance Raman optical cycling manipulates molecules to behave like efficient…

Atomic Physics · Physics 2021-08-26 J. C. Shaw , J. C. Schnaubelt , D. J. McCarron

Several centimeters below the skin lie multiple biomarkers, such as glucose, oxygenation, and blood flow. Monitoring these biomarkers regularly and in a non-invasive manner would enable early insight into metabolic status and vascular…

Medical Physics · Physics 2025-10-30 Talia Xu , Caitlin Smith , Charles Lo , Jami Shepherd , Gijs van Soest , Marco Zuniga

The random motion of molecules in living cells has consistently been reported to deviate from standard Brownian motion, a behavior coined as ``anomalous diffusion''. Fluorescence Correlation Spectroscopy (FCS) is a powerful method to…

Quantitative Methods · Quantitative Biology 2024-07-18 Nathan Quiblier , Jan-Michael Rye , Pierre Leclerc , Henri Truong , Abdelkrim Hannou , Laurent Héliot , Hugues Berry

We report on using a laser Doppler processing of Fourier-domain optical coherence tomography (OCT) data for the assessment of pulsatile blood flow in the choriocapillaris. Signal fluctuations in B-scans recorded at 2 kHz were analyzed by…

Lines provide the significantly richer geometric structural information about the environment than points, so lines are widely used in recent Visual Odometry (VO) works. Since VO with lines use line tracking results to locate and map, line…

Computer Vision and Pattern Recognition · Computer Science 2021-09-20 Meixiang Quan , Zheng Chai , Xiao Liu

Cells alter the path of light, a fact that leads to well-known aberrations in single cell or tissue imaging. Optical diffraction tomography (ODT) measures the biophysical property that causes these aberrations, the refractive index (RI).…

Quantitative Methods · Quantitative Biology 2017-08-14 Mirjam Schürmann , Gheorghe Cojoc , Salvatore Girardo , Elke Ulbricht , Jochen Guck , Paul Müller

Fluorescence Correlation Spectroscopy (FCS) is a powerful single-molecule technique which allows for measuring motion (diffusion, flow), concentration, and molecular interaction kinetics of fluorescent molecules from picomolar to micromolar…

Single-molecule fluorescence techniques have revolutionized our ability to study proteins. However, the presence of a fluorescent label can alter the protein structure and/or modify its reaction with other species. To avoid the need for a…

Biological Physics · Physics 2022-04-07 Aleksandr Barulin , Prithu Roy , Jean-Benoît Claude , Jérôme Wenger

Optical frequency combs (OFCs), consisting of a set of phase locked equally spaced laser frequency lines, have enabled a great leap in precision spectroscopy and metrology since seminal works of H\"ansch et al. . Nowadays, OFCs are…

Accurate focus quality assessment (FQA) in fluorescence microscopy is challenging due to stain-dependent optical variations that induce heterogeneous focus behavior across images. Existing methods, however, treat focus quality as a…

Image and Video Processing · Electrical Eng. & Systems 2026-04-03 Hyejin Park , Jiwon Yoon , Sumin Park , Suree Kim , Sinae Jang , Eunsoo Lee , Dongmin Kang , Dongbo Min

Optimal sensor placement (OSP) is critical for efficient, accurate monitoring, control, and inference in complex physical systems. We propose a machine-learning-based feature attribution (FA) framework to identify OSP for target…

Computational Engineering, Finance, and Science · Computer Science 2026-04-07 Sze Chai Leung , Di Zhou , H. Jane Bae

The recently demonstrated control over light distribution through turbid media based on real-time three-dimensional optoacoustic feedback has offered promising prospects to interferometrically focus light within scattering objects.…

Optics · Physics 2023-07-19 X. L. Dean-Ben , H. Estrada , A. Ozbek , D. Razansky

Learning to localize the sound source in videos without explicit annotations is a novel area of audio-visual research. Existing work in this area focuses on creating attention maps to capture the correlation between the two modalities to…

Computer Vision and Pattern Recognition · Computer Science 2022-11-08 Dennis Fedorishin , Deen Dayal Mohan , Bhavin Jawade , Srirangaraj Setlur , Venu Govindaraju

Various morphological and functional parameters of peripheral nerves and their vascular supply are indicative of pathological changes due to injury or disease. Based on recent improvements in optoacoustic image quality, we explore the…

Image and Video Processing · Electrical Eng. & Systems 2022-07-29 Dominik Jüstel , Hedwig Irl , Florian Hinterwimmer , Christoph Dehner , Walter Simson , Nassir Navab , Gerhard Schneider , Vasilis Ntziachristos

Multiplexed, real-time fluorescence detection at the single-molecule level is highly desirable to reveal the stoichiometry, dynamics, and interactions of individual molecular species within complex systems. However, traditionally…

Modern optical flow methods make use of salient scene feature points detected and matched within the scene as a basis for sparse-to-dense optical flow estimation. Current feature detectors however either give sparse, non uniform point…

Computer Vision and Pattern Recognition · Computer Science 2019-05-21 Felix Stephenson , Toby Breckon , Ioannis Katramados
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